##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_R1_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	85766175	
Filtered Sequences	0	
Sequence length	30-101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82362651709721	34.0	31.0	34.0	31.0	34.0
2	33.036136367279994	34.0	31.0	34.0	31.0	34.0
3	33.07815280324674	34.0	33.0	34.0	31.0	34.0
4	36.467279588952174	37.0	37.0	37.0	35.0	37.0
5	36.40309641883878	37.0	37.0	37.0	35.0	37.0
6	36.3794179581869	37.0	37.0	37.0	35.0	37.0
7	36.37433888126642	37.0	37.0	37.0	35.0	37.0
8	36.35536917671798	37.0	37.0	37.0	35.0	37.0
9	38.15821620819629	39.0	39.0	39.0	37.0	39.0
10-14	38.39974610969884	39.4	38.8	39.4	37.0	39.4
15-19	39.55753108495278	41.0	40.0	41.0	37.0	41.0
20-24	39.282580849618164	40.8	39.0	41.0	36.0	41.0
25-29	37.61367528399162	40.0	37.2	41.0	28.4	41.0
30-34	37.12983271320303	39.6	36.0	41.0	31.2	41.0
35-39	36.939191182338554	38.6	35.0	40.4	32.0	41.0
40-44	35.1695896629379	36.2	34.6	39.6	26.8	41.0
45-49	35.24891817473043	35.0	34.8	38.8	30.4	41.0
50-54	34.5580014096113	35.0	34.6	36.4	30.4	39.6
55-59	33.882964792236535	35.0	34.0	35.0	29.8	38.0
60-64	33.78072633331142	35.0	34.0	35.0	30.0	36.2
65-69	33.54080756544498	35.0	33.4	35.0	29.8	35.0
70-74	33.16864605915937	35.0	33.0	35.0	29.0	35.0
75-79	32.86916751333651	34.4	32.6	35.0	28.8	35.0
80-84	33.2095342675583	35.0	33.0	35.0	29.0	35.0
85-89	33.05582626590405	35.0	33.0	35.0	28.6	35.0
90-94	33.08448419986307	35.0	33.0	35.0	29.0	35.0
95-99	33.1663864212954	35.0	33.0	35.0	29.0	35.0
100-101	32.761635032405536	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	14.0
20	167.0
21	2295.0
22	16918.0
23	68935.0
24	180360.0
25	359244.0
26	597445.0
27	874920.0
28	1184124.0
29	1535285.0
30	1972360.0
31	2611546.0
32	3685024.0
33	5638354.0
34	9038186.0
35	1.6387602E7
36	2.6653912E7
37	1.2577999E7
38	2125347.0
39	255742.0
40	396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.286378925024934	23.137363885004785	13.846240665390521	25.730016524579767
2	30.978945966935456	24.05632069245794	19.84384908667854	25.12088425392806
3	28.318834979380508	19.82738346037662	21.60026657596113	30.253514984281736
4	30.67565100299411	19.819321040582476	19.982112524873884	29.522915431549535
5	31.1995958779787	20.654338379903265	20.046690901162375	28.099374840955655
6	29.672506672939537	19.121293446979536	17.823097509012147	33.38310237106878
7	32.74192069309375	18.88745184217438	19.191612544222707	29.17901492050916
8	28.55759511252542	19.535529012457417	19.96817393337175	31.93870194164541
9	30.66836547158597	17.89162219254852	19.287764669463222	32.15224766640228
10-14	30.82617998158322	19.257105105046172	19.210691447829166	30.706023465541442
15-19	31.020163119226176	18.820962824710065	18.992157122493737	31.166716933570026
20-24	30.983935903633498	18.885692834165557	19.008051371953528	31.122319890247418
25-29	31.93984707075431	18.80885004700331	18.712932927970822	30.53836995427155
30-34	31.64379624261229	18.76357636828899	18.632023770611404	30.96060361848732
35-39	31.180844812109104	18.85818401373009	18.87760829376309	31.083362880397726
40-44	31.981423136648683	18.770901015482757	18.620444857829384	30.627230990039173
45-49	31.371029828656035	18.747893896703214	18.69734835633822	31.18372791830253
50-54	31.20774533484732	18.858229286564058	18.846776995826026	31.087248382762596
55-59	31.247370894341692	18.95448671191864	18.81743812011829	30.980704273621374
60-64	31.319472672223263	18.723980254198743	18.68852758277851	31.26801949079948
65-69	31.102219662599946	18.855728948108638	18.8744530892584	31.167598300033017
70-74	31.229566782660527	18.964747195323152	18.82774933590176	30.977936686114553
75-79	31.281025971109504	18.7674429292765	18.726061519388836	31.225469580225163
80-84	31.056352274514786	18.86717603243788	18.906249382951852	31.170222310095486
85-89	31.139615091552344	18.988115156826588	18.884327911043965	30.9879418405771
90-94	31.168800668531883	18.794198722585886	18.79803304592496	31.23896756295727
95-99	30.90421304573455	18.90164534172648	19.00980602379857	31.184335588740396
100-101	31.44449136136398	18.814117429482113	18.77820855486323	30.963182654290677
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.0163954496047
2	56.099830220863524
3	58.57234996366225
4	60.19856643454364
5	59.29897071893436
6	63.05560904400832
7	61.920935613602914
8	60.49629705417083
9	62.82061313798826
10-14	61.532203447124665
15-19	62.1868800527962
20-24	62.10625579388092
25-29	62.47821702502586
30-34	62.6043998610996
35-39	62.26420769250682
40-44	62.60865412668786
45-49	62.554757746958565
50-54	62.29499371760991
55-59	62.22807516796307
60-64	62.58749216302275
65-69	62.26981796263297
70-74	62.20750346877508
75-79	62.50649555133466
80-84	62.226574584610276
85-89	62.127556932129444
90-94	62.40776823148916
95-99	62.08854863447495
100-101	62.407674015654656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	85.5
2	57.5
3	57.0
4	70.5
5	80.0
6	90.5
7	111.0
8	142.0
9	184.0
10	253.5
11	347.5
12	467.5
13	648.5
14	890.0
15	1225.0
16	1659.5
17	2277.5
18	3081.5
19	4213.0
20	5738.5
21	7562.0
22	9960.5
23	13153.5
24	17235.5
25	22487.5
26	28990.5
27	36826.5
28	46248.5
29	56952.0
30	68871.5
31	82735.5
32	97715.0
33	113588.0
34	130022.0
35	146487.5
36	163264.5
37	180643.0
38	198098.5
39	216506.5
40	236631.0
41	259971.5
42	289227.0
43	325384.5
44	371596.0
45	432197.0
46	511125.0
47	609690.0
48	730742.5
49	877304.5
50	1048263.0
51	1245529.0
52	1465282.0
53	1701861.0
54	1954333.0
55	2214456.5
56	2475942.5
57	2737497.5
58	2997431.0
59	3253281.0
60	3502826.5
61	3745411.5
62	3968484.0
63	4162083.5
64	4317062.5
65	4415760.0
66	4440142.0
67	4377841.5
68	4230990.0
69	3995634.5
70	3677530.5
71	3293666.5
72	2862954.5
73	2415862.5
74	1973386.0
75	1558820.5
76	1188967.5
77	870153.0
78	611738.0
79	412637.5
80	266815.5
81	164917.0
82	97258.5
83	55301.5
84	31220.0
85	18017.0
86	10459.5
87	6520.5
88	4220.5
89	2476.0
90	1517.5
91	1052.5
92	670.0
93	376.5
94	260.0
95	175.5
96	90.0
97	49.5
98	30.5
99	11.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.897035457160122E-5
3	2.3319216462667248E-6
4	2.098729481640052E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.640524076070782E-5
15-19	6.995764938800174E-7
20-24	0.001560521965681692
25-29	0.20500249661361253
30-34	0.2580774645096816
35-39	0.018695746245642648
40-44	0.3378461597043271
45-49	2.3468946866987237E-7
50-54	6.86540030379314E-4
55-59	0.10478324030816742
60-64	0.012586999860837722
65-69	0.012043867800761181
70-74	6.214454762251569E-5
75-79	0.0
80-84	3.999536976126504E-4
85-89	0.001839679094352982
90-94	0.006872943436788077
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	57759.0
32-33	58700.0
34-35	60227.0
36-37	65296.0
38-39	70425.0
40-41	68489.0
42-43	72728.0
44-45	59634.0
46-47	65863.0
48-49	72266.0
50-51	78331.0
52-53	82608.0
54-55	84098.0
56-57	87796.0
58-59	93365.0
60-61	97434.0
62-63	100605.0
64-65	110121.0
66-67	121250.0
68-69	128963.0
70-71	130791.0
72-73	138645.0
74-75	158074.0
76-77	105374.0
78-79	150358.0
80-81	179182.0
82-83	211624.0
84-85	238695.0
86-87	295235.0
88-89	311012.0
90-91	388550.0
92-93	505888.0
94-95	676648.0
96-97	1071639.0
98-99	1513955.0
100-101	7.8054547E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	2.030867993580404
#Duplication Level	Relative count
1	100.0
2	1.9744732101392695
3	0.01888227158830524
4	0.0158202816010125
5	0.012758291613719757
6	0.010206633290975806
7	0.008675638297329435
8	0.008165306632780645
9	0.005613648310036693
10++	0.011737628284622176
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE