##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_011.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3956744	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40933707108673	34.0	31.0	34.0	31.0	34.0
2	32.546221843010315	34.0	31.0	34.0	31.0	34.0
3	32.61274118315463	34.0	31.0	34.0	31.0	34.0
4	36.032737018113885	37.0	37.0	37.0	35.0	37.0
5	36.03490824779162	37.0	37.0	37.0	35.0	37.0
6	36.034444735368275	37.0	37.0	37.0	35.0	37.0
7	36.028656895669776	37.0	37.0	37.0	35.0	37.0
8	35.97819217012776	37.0	37.0	37.0	35.0	37.0
9	37.712181531077064	39.0	38.0	39.0	35.0	39.0
10-14	37.969486982225796	39.4	38.2	39.4	35.2	39.4
15-19	39.046757232714576	41.0	39.0	41.0	35.8	41.0
20-24	38.79634553056756	40.6	38.6	41.0	35.0	41.0
25-29	38.39002770965218	40.0	38.0	41.0	34.2	41.0
30-34	37.740025435054676	39.8	36.8	41.0	33.0	41.0
35-39	36.77256208640235	38.6	35.0	40.6	31.6	41.0
40-44	35.854792829659935	36.6	35.0	40.0	31.0	41.0
45-49	34.9057182875617	35.0	34.6	38.4	30.4	40.8
50-54	33.58528962197201	34.8	33.2	36.0	28.0	39.2
55-59	33.649780779347864	35.0	34.0	35.0	29.8	38.0
60-64	33.34525276338322	35.0	34.0	35.0	29.4	36.2
65-69	33.050135616557455	35.0	33.8	35.0	29.0	35.0
70-74	32.778168868140064	35.0	33.0	35.0	28.8	35.0
75-79	32.5370892835119	35.0	33.0	35.0	27.2	35.0
80-84	32.258071080666326	35.0	33.0	35.0	27.0	35.0
85-89	31.977021308429357	35.0	33.0	35.0	26.6	35.0
90-94	31.599403044523473	35.0	33.0	35.0	24.4	35.0
95-99	31.04077408091097	35.0	32.6	35.0	23.4	35.0
100-101	29.904164510011263	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6439.0
3	1232.0
4	1665.0
5	2100.0
6	2751.0
7	3683.0
8	4233.0
9	4901.0
10	5160.0
11	5473.0
12	5721.0
13	5587.0
14	5651.0
15	5580.0
16	5989.0
17	6736.0
18	7624.0
19	8760.0
20	10190.0
21	12296.0
22	15005.0
23	18313.0
24	22535.0
25	27557.0
26	33421.0
27	40714.0
28	49701.0
29	61639.0
30	76138.0
31	96569.0
32	124973.0
33	172791.0
34	277039.0
35	648487.0
36	1354145.0
37	695572.0
38	115051.0
39	15275.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.22933215205339	22.72874151207879	13.56431502956234	26.477611306305487
2	31.378255667296113	24.096312426734524	19.133780857470274	25.391651048499085
3	28.700773962887205	19.635926441919914	20.923344551286103	30.73995504390678
4	30.99712528159346	19.350849647423498	19.420631730115268	30.231393340867776
5	31.41724404516016	20.618365919845726	19.82539851883013	28.138991516163987
6	29.69482230007257	19.020946035955806	17.605989651728514	33.6782420122431
7	32.90611680303254	18.719336643540732	19.015788601911524	29.358757951515198
8	28.623333096440206	19.5205670867431	19.778890191941603	32.07720962487509
9	30.715700258795337	17.852299727039345	19.139193745404175	32.29280626876114
10-14	30.885517406259353	19.243084580792413	19.099805811378797	30.771592201569437
15-19	31.054128432155686	18.807543919805713	18.95143642963322	31.18689121840538
20-24	30.994194267922165	18.868727842866498	18.97551840721585	31.16155948199549
25-29	31.15264183226482	18.99713481101225	18.881053930866475	30.969169425856453
30-34	31.281255939952796	18.741976718191474	18.70976096782971	31.267006374026025
35-39	31.092871621455938	18.822432674587848	18.86634409678047	31.218351607175748
40-44	31.25424092466136	18.890204552226606	18.76866550316236	31.086889019949677
45-49	31.37385559133483	18.65606981783239	18.63246617978834	31.33760841104444
50-54	31.158767697155053	18.76081326741937	18.808225942377472	31.272193093048106
55-59	31.289550312969965	18.840949258569626	18.747739973088713	31.121760455371692
60-64	31.37959406987666	18.641312133149466	18.623833010793483	31.355260786180395
65-69	31.17003778864642	18.763083990270786	18.786097862282723	31.280780358800065
70-74	31.291428395814876	18.855412311246596	18.738669640026075	31.114489652912454
75-79	31.398956129938743	18.661009733546948	18.604152612035776	31.335881524478538
80-84	31.182874024614225	18.78768298996158	18.780873873064614	31.248569112359583
85-89	31.31074907673686	18.83513413157562	18.73945403559326	31.11466275609425
90-94	31.4102473376777	18.631090182313727	18.611553680141206	31.347108799867364
95-99	31.22493599852313	18.753170754787753	18.758483569904413	31.263409676784708
100-101	31.43499731078436	18.93564181265266	18.73062703207648	30.898733844486504
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.706943458358865
2	56.7699067157952
3	59.44072900679398
4	61.22851862246124
5	59.556235561324144
6	63.37306431231568
7	62.26487475454774
8	60.7005427213153
9	63.008506527556484
10-14	61.65710960782879
15-19	62.24101965056107
20-24	62.15575374991765
25-29	62.12181125812128
30-34	62.54826231397882
35-39	62.31122322863168
40-44	62.341129944611026
45-49	62.711464002379266
50-54	62.43096079020316
55-59	62.411310768341664
60-64	62.734854856057055
65-69	62.45081814744648
70-74	62.40591804872733
75-79	62.73483765441728
80-84	62.431443136973805
85-89	62.42541183283111
90-94	62.75735613754506
95-99	62.48834567530783
100-101	62.33373115527087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	101.0
2	55.0
3	41.5
4	29.5
5	26.0
6	29.5
7	36.0
8	44.0
9	46.5
10	50.5
11	69.0
12	81.5
13	91.5
14	116.5
15	134.5
16	155.5
17	191.0
18	225.5
19	272.5
20	361.5
21	446.5
22	539.5
23	711.0
24	906.0
25	1147.5
26	1471.5
27	1813.0
28	2193.0
29	2723.0
30	3287.0
31	3864.5
32	4526.5
33	5258.5
34	6042.5
35	6798.0
36	7535.0
37	8190.0
38	8908.5
39	9731.5
40	10720.0
41	11824.5
42	13056.0
43	14582.0
44	16686.0
45	19439.0
46	22655.0
47	26952.0
48	32378.0
49	38829.0
50	46603.5
51	55431.5
52	65059.5
53	75607.5
54	86830.0
55	98348.0
56	110061.0
57	121624.5
58	133018.5
59	144214.0
60	155328.0
61	166409.5
62	176475.0
63	185074.0
64	192221.5
65	197311.0
66	199033.0
67	197028.0
68	190998.0
69	180802.5
70	167235.0
71	149897.5
72	130309.5
73	110464.0
74	91045.5
75	72451.0
76	55177.0
77	40386.5
78	28420.0
79	19111.0
80	12218.0
81	7382.0
82	4226.5
83	2342.5
84	1235.0
85	640.5
86	372.0
87	238.0
88	165.0
89	125.5
90	100.0
91	77.5
92	50.0
93	38.0
94	31.5
95	24.0
96	17.5
97	16.5
98	12.0
99	6.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043267899060439596
2	0.049232399164565614
3	0.04483484400304897
4	0.03978018289785743
5	0.03088397935272032
6	0.018222053284215507
7	0.05835606245943634
8	0.07028506266768839
9	0.08661161803745707
10-14	0.038349713805088226
15-19	0.039663925692438026
20-24	0.02435841186591804
25-29	0.02663300936325423
30-34	0.03881474262676585
35-39	0.02662290004104385
40-44	0.016407429947451743
45-49	0.014511932033004913
50-54	0.021224521980699282
55-59	0.012277771824510255
60-64	5.964500104126019E-4
65-69	0.0
70-74	1.8702246089208704E-4
75-79	0.00403361956194285
80-84	0.007172564108266797
85-89	4.3470085504647253E-4
90-94	0.0012131186652459699
95-99	0.006894557747481263
100-101	0.0050546611051915415
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3956744.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2113837389435766
#Duplication Level	Relative count
1	100.0
2	0.15173746913920286
3	0.0020031349061280903
4	5.007837265320226E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.002503918632660113
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	307440	3.301893	4.0599847	8
TTTTT	286195	3.1938062	3.7622612	10-14
>>END_MODULE