##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45797525	34.0	31.0	34.0	31.0	34.0
2	32.636875	34.0	31.0	34.0	31.0	34.0
3	32.66746575	34.0	31.0	34.0	31.0	34.0
4	36.0833865	37.0	37.0	37.0	35.0	37.0
5	36.086673	37.0	37.0	37.0	35.0	37.0
6	35.986171	37.0	37.0	37.0	35.0	37.0
7	36.0281125	37.0	37.0	37.0	35.0	37.0
8	36.02466675	37.0	37.0	37.0	35.0	37.0
9	37.7561885	39.0	38.0	39.0	35.0	39.0
10-14	38.026107249999995	39.4	38.2	39.4	35.2	39.4
15-19	39.12289125	41.0	39.0	41.0	36.0	41.0
20-24	38.90123725	40.6	39.0	41.0	35.2	41.0
25-29	38.526252799999995	40.0	38.0	41.0	34.6	41.0
30-34	37.85167945	40.0	37.0	41.0	33.2	41.0
35-39	36.882244150000005	38.6	35.0	40.6	32.2	41.0
40-44	35.93813715	36.6	35.0	40.0	31.0	41.0
45-49	34.75819975	35.0	34.2	38.4	29.8	40.8
50-54	33.618497149999996	34.8	33.2	36.0	28.0	39.2
55-59	33.747364499999996	35.0	34.0	35.0	30.0	38.0
60-64	33.4121554	35.0	34.0	35.0	30.0	36.2
65-69	33.1080664	35.0	34.0	35.0	29.0	35.0
70-74	32.83662489999999	35.0	33.2	35.0	29.0	35.0
75-79	32.57232685	35.0	33.0	35.0	27.8	35.0
80-84	32.28854725	35.0	33.0	35.0	27.0	35.0
85-89	31.975057999999997	35.0	33.0	35.0	26.6	35.0
90-94	31.550541900000002	35.0	33.0	35.0	24.4	35.0
95-99	30.974627699999996	35.0	32.6	35.0	23.0	35.0
100-101	29.805533875000002	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8051.0
3	1189.0
4	1508.0
5	1900.0
6	2486.0
7	3117.0
8	3828.0
9	4605.0
10	4933.0
11	5066.0
12	5344.0
13	5302.0
14	5270.0
15	5542.0
16	5827.0
17	6509.0
18	7384.0
19	8568.0
20	10045.0
21	12023.0
22	14467.0
23	17804.0
24	21512.0
25	26657.0
26	32357.0
27	39315.0
28	48843.0
29	60166.0
30	75506.0
31	96108.0
32	126550.0
33	178430.0
34	288947.0
35	670642.0
36	1361945.0
37	700993.0
38	115934.0
39	15273.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.263079834552435	22.754944061871704	13.55777804687414	26.424198056701716
2	31.385072707277985	24.083442908241075	19.140699185913693	25.39078519856725
3	28.71591312227519	19.612210556443152	20.937182423307497	30.734693897974164
4	31.000752125285803	19.37539811956164	19.3729953233075	30.25085443184506
5	31.4083597779577	20.62630675464116	19.830604328138957	28.134729139262184
6	29.780998217165376	19.101263104034732	17.557366371263168	33.56037230753672
7	32.85390964997698	18.713398165116484	19.03421859206079	29.398473592845754
8	28.57375421231706	19.51281759086145	19.850904951593574	32.06252324522792
9	30.753683210810095	17.823223913971255	19.172407125783177	32.25068574943547
10-14	30.88580112135594	19.215055848830907	19.130832783833053	30.768310245980096
15-19	31.07837204564028	18.809772418690592	18.951639440954047	31.160216094715082
20-24	30.97566626302253	18.869184271889356	18.965923761296196	31.189225703791916
25-29	31.13256694352806	18.976088169751748	18.897058536566327	30.99428635015386
30-34	31.278001547391533	18.735986243361932	18.717576118333756	31.268436090912783
35-39	31.095766212880193	18.827101107156434	18.84123071976847	31.235901960194905
40-44	31.241107123536104	18.875014920472008	18.773025427485905	31.110852528505983
45-49	31.335335694224497	18.640027854120795	18.71873145107275	31.305905000581962
50-54	31.16253624388648	18.756575500565962	18.82055273549578	31.26033552005178
55-59	31.272012461716315	18.82102313236611	18.762590074915792	31.144374331001785
60-64	31.36976597941809	18.65881653058579	18.61606651562328	31.355350974372843
65-69	31.182554622305886	18.762215068404384	18.77492513958078	31.28030516970895
70-74	31.29785595520609	18.822624876111647	18.727434219296114	31.15208494938615
75-79	31.39884962905502	18.651876735484187	18.60941559327946	31.339858042181334
80-84	31.193272903116036	18.732723869889153	18.781270827004693	31.29273239999012
85-89	31.317289999999996	18.83564	18.710835	31.136235
90-94	31.43108	18.63643	18.586435	31.346055
95-99	31.244377871080513	18.742248551487524	18.762624001920514	31.25074957551145
100-101	31.44786598729797	18.942524297966372	18.700163507578885	30.909446207156776
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.687277891254155
2	56.77585790584523
3	59.45060702024936
4	61.25160655713087
5	59.54308891721989
6	63.3413705247021
7	62.25238324282273
8	60.63627745754497
9	63.00436896024557
10-14	61.65411136733604
15-19	62.23858814035537
20-24	62.16489196681445
25-29	62.126853293681926
30-34	62.54643763830431
35-39	62.33166817307511
40-44	62.35195965204209
45-49	62.641240694806456
50-54	62.42287176393826
55-59	62.41638679271809
60-64	62.72511695379094
65-69	62.46285979201483
70-74	62.44994090459224
75-79	62.73870767123636
80-84	62.486005303106154
85-89	62.453525
90-94	62.777134999999994
95-99	62.49512744659196
100-101	62.35731219445475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	57.5
2	19.5
3	14.0
4	15.0
5	17.5
6	24.5
7	27.0
8	31.5
9	43.5
10	51.0
11	62.5
12	83.0
13	100.5
14	132.0
15	167.0
16	208.0
17	290.0
18	340.0
19	380.5
20	439.0
21	524.5
22	654.5
23	777.5
24	1006.5
25	1222.5
26	1500.0
27	1931.5
28	2346.0
29	2843.5
30	3393.5
31	3969.5
32	4665.0
33	5382.0
34	6090.0
35	6861.5
36	7676.5
37	8368.5
38	9069.0
39	9912.5
40	10819.0
41	11865.5
42	13061.5
43	14644.5
44	16876.0
45	19547.5
46	22952.0
47	27413.5
48	32748.5
49	39282.5
50	47110.0
51	56251.5
52	66050.0
53	76505.0
54	87729.5
55	99010.5
56	110760.5
57	122747.5
58	134411.0
59	146084.0
60	157501.0
61	167955.0
62	178171.5
63	187412.5
64	194808.0
65	199436.0
66	200819.0
67	198859.0
68	192930.0
69	182993.0
70	168539.5
71	150925.0
72	131784.5
73	111965.5
74	91981.5
75	72616.5
76	55407.5
77	40783.0
78	28781.5
79	19440.5
80	12462.5
81	7633.0
82	4393.5
83	2356.0
84	1245.5
85	714.5
86	424.5
87	260.0
88	181.5
89	146.0
90	106.0
91	87.5
92	72.0
93	54.0
94	41.5
95	27.5
96	21.5
97	17.5
98	14.5
99	9.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0771
2	0.087375
3	0.07335
4	0.116375
5	0.050575
6	0.13094999999999998
7	0.154425
8	0.18112499999999998
9	0.1822
10-14	0.09862499999999999
15-19	0.096585
20-24	0.077
25-29	0.06913000000000001
30-34	0.109315
35-39	0.032645
40-44	0.053430000000000005
45-49	0.036335
50-54	0.050385
55-59	0.06513
60-64	3.5E-5
65-69	5.6E-4
70-74	6.9E-4
75-79	0.00269
80-84	0.024630000000000003
85-89	0.0
90-94	0.0
95-99	0.02675
100-101	0.019925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1903781579789007
#Duplication Level	Relative count
1	100.0
2	0.12015199227022183
3	0.0020025332045036973
4	0.0010012666022518487
5	5.006333011259243E-4
6	0.0
7	5.006333011259243E-4
8	0.0
9	0.0
10++	0.0025031665056296213
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	302800	3.2163184	4.056142	1
TTTTT	289930	3.199834	3.526843	10-14
>>END_MODULE