##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5677985	34.0	31.0	34.0	31.0	34.0
2	32.739742	34.0	31.0	34.0	31.0	34.0
3	32.78837775	34.0	31.0	34.0	31.0	34.0
4	36.16150325	37.0	37.0	37.0	35.0	37.0
5	36.03282875	37.0	37.0	37.0	35.0	37.0
6	36.11277425	37.0	37.0	37.0	35.0	37.0
7	36.13575925	37.0	37.0	37.0	35.0	37.0
8	36.12040525	37.0	37.0	37.0	35.0	37.0
9	37.88329575	39.0	39.0	39.0	37.0	39.0
10-14	38.008064049999994	39.4	38.6	39.4	35.2	39.4
15-19	39.2475071	41.0	39.8	41.0	36.6	41.0
20-24	38.346113849999995	40.6	38.6	41.0	33.0	41.0
25-29	38.446675150000004	40.0	38.0	41.0	34.8	41.0
30-34	37.946355749999995	40.0	37.2	41.0	33.4	41.0
35-39	36.653674699999996	38.6	35.2	40.6	31.6	41.0
40-44	35.77712964999999	36.6	35.0	40.0	30.6	41.0
45-49	34.72305955	35.0	34.6	38.4	29.8	40.8
50-54	33.613101799999995	34.8	33.2	36.0	28.6	39.2
55-59	33.73150885	35.0	34.0	35.0	30.0	38.0
60-64	33.38795655	35.0	34.0	35.0	30.0	36.2
65-69	33.0601367	35.0	34.0	35.0	29.2	35.0
70-74	32.74524815000001	35.0	33.2	35.0	29.0	35.0
75-79	32.265866599999995	35.0	33.0	35.0	27.0	35.0
80-84	32.0951318	35.0	33.0	35.0	27.0	35.0
85-89	31.766279149999995	35.0	33.0	35.0	25.2	35.0
90-94	31.30612815	35.0	32.8	35.0	24.0	35.0
95-99	30.633507549999997	35.0	31.6	35.0	20.6	35.0
100-101	29.340173	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10156.0
3	1537.0
4	1997.0
5	2385.0
6	2892.0
7	3727.0
8	4362.0
9	4974.0
10	5211.0
11	5849.0
12	5757.0
13	5994.0
14	6001.0
15	6247.0
16	6439.0
17	7186.0
18	8108.0
19	9172.0
20	10700.0
21	12427.0
22	14849.0
23	17745.0
24	21335.0
25	26090.0
26	31899.0
27	38438.0
28	46824.0
29	58670.0
30	73193.0
31	94252.0
32	126957.0
33	183910.0
34	318249.0
35	736539.0
36	1319723.0
37	647149.0
38	109005.0
39	14008.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.19657888611055	22.755438414693668	13.603683429323763	26.444299269872023
2	31.424197314046225	24.08141717591618	19.115974718962605	25.37841079107499
3	28.68792126121017	19.65406369170418	20.92809299049878	30.729922056586872
4	30.94682335433012	19.38830821338358	19.438907809838653	30.225960622447644
5	31.63651914411681	20.608699111901956	19.801300855567895	27.953480888413335
6	29.664345210017718	19.10026529502429	17.61214949059149	33.6232400043665
7	32.845000146502464	18.724441894494685	19.08553915534946	29.345018803653396
8	28.62360888141654	19.483362686397804	19.856665533624184	32.03636289856147
9	30.70415436496147	17.857316418812253	19.17613102624904	32.26239818997724
10-14	30.95854940435881	19.276687516544477	19.088581077644605	30.67618200145211
15-19	31.101347121832646	18.827438606379776	18.9517713714773	31.11944290031028
20-24	31.229789221859207	18.876865532332392	18.918749706600583	30.974595539207822
25-29	31.11850873975626	19.017110281667886	18.912371430420226	30.952009548155623
30-34	31.242183465003254	18.766112450762716	18.735522200110907	31.25618188412313
35-39	31.244623027751466	18.902734214620242	18.785635846838517	31.067006910789775
40-44	31.280928156161764	18.942414548998947	18.7435746941647	31.033082600674593
45-49	31.361899337342148	18.739781908997923	18.642555736576806	31.255763017083122
50-54	31.138975302551785	18.759118758933628	18.83436798729004	31.26753795122455
55-59	31.27141659948249	18.844991221061367	18.76581638874993	31.11777579070622
60-64	31.361802311029013	18.639282693202503	18.628412650809338	31.370502344959146
65-69	31.165771748657622	18.765798148697225	18.797958196937294	31.270471905707858
70-74	31.28598241833452	18.846379580331664	18.731972852790076	31.135665148543744
75-79	31.44434374713726	18.678701539448696	18.59299937474773	31.283955338666313
80-84	31.167307849291802	18.76099927651871	18.78299782955613	31.288695044633354
85-89	31.32805494898408	18.82719807111354	18.729257743013438	31.115489236888944
90-94	31.42617	18.633454999999998	18.59451	31.345865
95-99	31.250401098721632	18.73418020373289	18.749941539001604	31.265477158543874
100-101	31.549344806052737	18.91634469876484	18.660179208305333	30.874131286877088
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.64087815598257
2	56.80260810512121
3	59.417843317797036
4	61.172783976777765
5	59.59000003253014
6	63.28758521438422
7	62.19001895015586
8	60.659971779978015
9	62.966552554938716
10-14	61.63473140581092
15-19	62.22079002214292
20-24	62.20438476106702
25-29	62.07051828791188
30-34	62.49836534912638
35-39	62.311629938541245
40-44	62.31401075683636
45-49	62.61766235442528
50-54	62.40651325377633
55-59	62.38919239018871
60-64	62.73230465598816
65-69	62.436243654365484
70-74	62.421647566878256
75-79	62.72829908580357
80-84	62.45600289392515
85-89	62.44354418587302
90-94	62.772035
95-99	62.51587825726551
100-101	62.42347609292983
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	98.5
2	55.0
3	37.0
4	32.5
5	32.0
6	27.0
7	35.0
8	51.5
9	57.5
10	66.5
11	86.5
12	120.5
13	152.5
14	210.5
15	278.0
16	317.0
17	356.0
18	408.5
19	482.0
20	534.0
21	586.0
22	676.5
23	833.0
24	1011.5
25	1264.0
26	1588.0
27	1947.5
28	2334.5
29	2785.0
30	3357.5
31	4010.0
32	4716.5
33	5415.0
34	6120.5
35	6898.0
36	7642.0
37	8380.5
38	9220.5
39	10046.5
40	10952.5
41	12002.5
42	13261.5
43	14947.5
44	17018.0
45	19724.0
46	23099.0
47	27591.5
48	33088.0
49	39444.0
50	47294.5
51	56056.5
52	65936.5
53	76777.0
54	88058.0
55	99628.0
56	111154.5
57	123312.0
58	135312.0
59	146731.0
60	157944.5
61	168312.5
62	178021.5
63	186934.5
64	194076.0
65	198459.5
66	199989.5
67	197765.0
68	191436.0
69	181332.0
70	167637.5
71	150611.0
72	131234.5
73	110554.5
74	90622.0
75	72329.0
76	55197.5
77	40809.0
78	29024.0
79	19682.0
80	12866.0
81	8025.0
82	4794.0
83	2850.5
84	1730.5
85	1072.0
86	729.5
87	533.5
88	400.0
89	296.5
90	221.5
91	173.0
92	144.0
93	123.0
94	104.0
95	72.5
96	50.0
97	45.5
98	31.0
99	19.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12025
2	0.126725
3	0.11022499999999999
4	0.147425
5	0.092675
6	0.1489
7	0.172325
8	0.194975
9	0.20015
10-14	0.11772
15-19	0.114825
20-24	0.09353
25-29	0.089605
30-34	0.13158
35-39	0.054115
40-44	0.07285
45-49	0.057775
50-54	0.072065
55-59	0.081885
60-64	3.9E-4
65-69	1.5000000000000001E-4
70-74	0.0015099999999999998
75-79	0.00836
80-84	0.038880000000000005
85-89	3.35E-4
90-94	0.0
95-99	0.040195
100-101	0.035325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.19491716020691674
#Duplication Level	Relative count
1	100.0
2	0.15022909937654924
3	0.0015022909937654923
4	5.007636645884975E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007636645884975E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	320375	3.3870401	4.0584035	1
TTTTT	298880	3.3041995	3.698502	9
>>END_MODULE