##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55084525	34.0	31.0	34.0	31.0	34.0
2	32.69225025	34.0	31.0	34.0	31.0	34.0
3	32.742508	34.0	31.0	34.0	31.0	34.0
4	36.131475	37.0	37.0	37.0	35.0	37.0
5	36.13287125	37.0	37.0	37.0	35.0	37.0
6	36.14598725	37.0	37.0	37.0	35.0	37.0
7	36.15222075	37.0	37.0	37.0	35.0	37.0
8	36.12669775	37.0	37.0	37.0	35.0	37.0
9	37.88272825	39.0	39.0	39.0	35.0	39.0
10-14	38.1424405	39.4	38.4	39.4	35.2	39.4
15-19	39.308112	41.0	39.8	41.0	36.2	41.0
20-24	39.0945105	41.0	39.0	41.0	35.6	41.0
25-29	38.706244749999996	40.2	38.2	41.0	35.0	41.0
30-34	38.060154	40.0	37.2	41.0	33.6	41.0
35-39	37.069451900000004	38.8	35.2	40.8	32.6	41.0
40-44	36.12914585	37.0	35.0	40.0	31.8	41.0
45-49	35.172464149999996	35.0	35.0	38.4	31.0	41.0
50-54	33.8460798	35.0	33.4	36.0	29.0	39.2
55-59	33.921926049999996	35.0	34.8	35.0	30.8	38.0
60-64	33.5831311	35.0	34.4	35.0	30.4	36.4
65-69	33.29336475	35.0	34.0	35.0	30.0	35.0
70-74	33.041878049999994	35.0	34.0	35.0	29.2	35.0
75-79	32.78631944999999	35.0	33.4	35.0	29.0	35.0
80-84	32.5353893	35.0	33.0	35.0	28.2	35.0
85-89	32.262359599999996	35.0	33.0	35.0	27.0	35.0
90-94	31.90790895	35.0	33.0	35.0	26.6	35.0
95-99	31.37140635	35.0	33.0	35.0	24.2	35.0
100-101	30.304636125000002	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7422.0
3	1168.0
4	1430.0
5	1739.0
6	2370.0
7	2841.0
8	3556.0
9	4131.0
10	4541.0
11	4639.0
12	4765.0
13	4790.0
14	4877.0
15	4864.0
16	5190.0
17	5993.0
18	6504.0
19	7497.0
20	8807.0
21	10488.0
22	12999.0
23	15631.0
24	19142.0
25	23547.0
26	28845.0
27	35156.0
28	43773.0
29	54101.0
30	67864.0
31	86659.0
32	114146.0
33	161495.0
34	261415.0
35	632935.0
36	1422131.0
37	774947.0
38	129789.0
39	17746.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.20248929785196	22.754351589285886	13.582301508891458	26.4608576039707
2	31.39854549806139	24.095818175491075	19.141028205540966	25.364608120906567
3	28.697975172132022	19.67914085072261	20.919933417833636	30.702950559311727
4	30.987993898070542	19.357780435676162	19.42384128174563	30.23038438450767
5	31.434627941986083	20.61000685229364	19.824584287092325	28.130780918627952
6	29.664566848384116	19.086335173019272	17.626692579849998	33.622405398746615
7	32.84514971806314	18.7255448573882	19.081258296582615	29.348047127966044
8	28.59508280106253	19.518102794962758	19.863323016090845	32.023491387883865
9	30.706246753207754	17.85833325826299	19.18740359717055	32.2480163913587
10-14	30.854923478415273	19.24206930270886	19.149830750935653	30.753176467940214
15-19	31.038389102012182	18.836182763252282	18.97171437257273	31.15371376216281
20-24	30.96148221409198	18.887381310723562	18.99509483415643	31.156041641028033
25-29	31.086230963499272	18.98806861353544	18.924282537911736	31.00141788505355
30-34	31.272600648755972	18.75690917506438	18.73224096301659	31.238249213163062
35-39	31.091397116828333	18.828828716199745	18.88342670115504	31.196347465816878
40-44	31.24148027995134	18.902070533856623	18.77543540513391	31.081013781058132
45-49	31.35094133577076	18.667044347685213	18.65677395741038	31.325240359133648
50-54	31.154470365707752	18.77126794440812	18.816978601101887	31.257283088782238
55-59	31.244707726491804	18.831242992597343	18.787254680277425	31.136794600633426
60-64	31.363000000000003	18.65576	18.631819999999998	31.34942
65-69	31.166470707794446	18.757551583266853	18.818761751594817	31.25721595734388
70-74	31.26234	18.838404999999998	18.767644999999998	31.13161
75-79	31.357306357306356	18.662688662688662	18.625823625823624	31.354181354181353
80-84	31.18104426657205	18.75273942896675	18.795005261603652	31.271211042857544
85-89	31.28827	18.84334	18.746645	31.121745
90-94	31.384794999999997	18.644635	18.625485	31.345085
95-99	31.19854	18.76116	18.79352	31.24678
100-101	31.431790366397305	18.936641942845586	18.710803793560906	30.920763897196203
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.663346901822656
2	56.763153618967955
3	59.400925731443756
4	61.218378282578215
5	59.565408860614035
6	63.28697224713073
7	62.19319684602919
8	60.6185741889464
9	62.95426314456646
10-14	61.60809994635549
15-19	62.19210286417499
20-24	62.117523855120005
25-29	62.087648848552824
30-34	62.51084986191904
35-39	62.28774458264521
40-44	62.32249406100947
45-49	62.67618169490441
50-54	62.41175345449
55-59	62.38150232712524
60-64	62.71242
65-69	62.423686665138334
70-74	62.39395
75-79	62.71148771148771
80-84	62.452255309429596
85-89	62.410014999999994
90-94	62.72988
95-99	62.44532
100-101	62.352554263593504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	80.5
2	45.0
3	36.5
4	31.0
5	37.5
6	40.0
7	43.0
8	51.5
9	51.5
10	54.5
11	65.0
12	73.5
13	84.5
14	96.0
15	123.0
16	143.5
17	182.0
18	233.5
19	278.0
20	348.5
21	433.5
22	550.0
23	720.0
24	915.0
25	1148.0
26	1485.5
27	1852.5
28	2252.0
29	2735.5
30	3284.5
31	3950.5
32	4648.5
33	5367.0
34	6115.5
35	6895.5
36	7679.5
37	8463.0
38	9221.0
39	10069.0
40	10971.5
41	11921.5
42	13235.0
43	14908.0
44	16971.5
45	19578.5
46	23114.0
47	27728.0
48	33253.0
49	39500.5
50	47160.5
51	56234.0
52	65818.5
53	76261.5
54	88024.5
55	99896.0
56	111259.5
57	122910.5
58	134656.5
59	146244.5
60	157797.5
61	168834.5
62	178869.5
63	187339.5
64	194185.0
65	199005.0
66	200808.0
67	199055.0
68	192836.0
69	182592.5
70	168003.0
71	150401.0
72	131680.5
73	111802.0
74	91716.5
75	72386.0
76	55076.0
77	40595.0
78	28654.5
79	19162.5
80	12291.0
81	7453.0
82	4198.0
83	2261.5
84	1233.0
85	712.5
86	420.5
87	268.0
88	184.5
89	136.5
90	108.5
91	85.5
92	63.0
93	49.0
94	36.5
95	33.5
96	25.5
97	18.0
98	13.0
99	8.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14855000000000002
2	0.14107499999999998
3	0.13842500000000002
4	0.12995
5	0.142925
6	0.050875
7	0.052974999999999994
8	0.078275
9	0.0937
10-14	0.036375
15-19	0.034390000000000004
20-24	0.012554999999999998
25-29	0.009525
30-34	0.03329
35-39	0.014625
40-44	0.00405
45-49	0.0038000000000000004
50-54	0.012375
55-59	0.00753
60-64	0.0
65-69	2.75E-4
70-74	0.0
75-79	9.999999999999999E-5
80-84	0.00197
85-89	0.0
90-94	0.0
95-99	0.0
100-101	2.875E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.22037548784937244
#Duplication Level	Relative count
1	100.0
2	0.15675708161384672
3	0.003505749429063664
4	0.0010016426940181898
5	5.008213470090949E-4
6	0.0
7	0.0
8	0.0
9	5.008213470090949E-4
10++	0.0020032853880363796
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	296735	3.2630558	3.8429155	10-14
AAAAA	305590	3.245127	4.4038568	1
>>END_MODULE