##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5753895	34.0	31.0	34.0	31.0	34.0
2	32.72467325	34.0	31.0	34.0	31.0	34.0
3	32.7707995	34.0	31.0	34.0	31.0	34.0
4	36.17279825	37.0	37.0	37.0	35.0	37.0
5	36.16224575	37.0	37.0	37.0	35.0	37.0
6	36.17115975	37.0	37.0	37.0	35.0	37.0
7	36.1713695	37.0	37.0	37.0	35.0	37.0
8	36.1432065	37.0	37.0	37.0	35.0	37.0
9	37.89739325	39.0	39.0	39.0	35.0	39.0
10-14	38.172531549999995	39.4	38.6	39.4	35.4	39.4
15-19	39.33432725	41.0	40.0	41.0	36.6	41.0
20-24	39.11351534999999	41.0	39.0	41.0	35.8	41.0
25-29	38.718440099999995	40.2	38.4	41.0	35.0	41.0
30-34	38.03937075	40.0	37.2	41.0	33.8	41.0
35-39	37.0347706	38.6	35.2	40.8	32.6	41.0
40-44	36.09184465	37.0	35.0	40.0	31.8	41.0
45-49	35.120740749999996	35.0	35.0	38.4	31.0	41.0
50-54	33.76852435	34.8	33.2	36.0	28.8	39.2
55-59	33.88900075	35.0	34.8	35.0	30.8	38.0
60-64	33.531027550000005	35.0	34.2	35.0	30.2	36.4
65-69	33.1950466	35.0	34.0	35.0	29.6	35.0
70-74	32.9242661	35.0	33.8	35.0	29.0	35.0
75-79	32.679787700000006	35.0	33.0	35.0	29.0	35.0
80-84	32.364765999999996	35.0	33.0	35.0	27.0	35.0
85-89	32.055970099999996	35.0	33.0	35.0	27.0	35.0
90-94	31.66369935	35.0	33.0	35.0	25.0	35.0
95-99	31.080534	35.0	32.8	35.0	23.4	35.0
100-101	29.885865125000002	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7990.0
3	1189.0
4	1547.0
5	1881.0
6	2426.0
7	3132.0
8	3749.0
9	4498.0
10	4640.0
11	4838.0
12	5071.0
13	5033.0
14	5243.0
15	5405.0
16	5819.0
17	6541.0
18	7174.0
19	8076.0
20	9434.0
21	11239.0
22	13590.0
23	16363.0
24	19514.0
25	24237.0
26	29416.0
27	35850.0
28	44520.0
29	54691.0
30	68414.0
31	87505.0
32	116299.0
33	163742.0
34	271253.0
35	655613.0
36	1401627.0
37	748778.0
38	126559.0
39	17039.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.25001941086157	22.736155046448314	13.574328572036837	26.43949697065328
2	31.377747877876494	24.068641312474313	19.140425704575563	25.413185105073637
3	28.70871817040419	19.62427996845248	20.908947238051777	30.758054623091553
4	30.982223109331773	19.348894425810414	19.44977113658852	30.219111328269292
5	31.407352052883013	20.607916223821196	19.85036247381744	28.134369249478354
6	29.706217619433573	19.032751899467996	17.6376810796117	33.623349401486735
7	32.87546720543872	18.692072596046376	19.075950187783356	29.35651001073155
8	28.576279445854137	19.534160083077307	19.819707020778594	32.06985345028996
9	30.74393552869307	17.867756482468895	19.179602719463762	32.20870526937428
10-14	30.86359400647855	19.239733249291298	19.130173921365966	30.766498822864186
15-19	31.06517057243108	18.806514769887244	18.972060273478668	31.15625438420301
20-24	30.95173560823281	18.861923145337567	19.00838827068128	31.17795297574834
25-29	31.111831498904568	18.98371216986324	18.912682978596827	30.991773352635366
30-34	31.243960782607992	18.749139399790447	18.742687557288885	31.264212260312675
35-39	31.093499267008067	18.83694943854324	18.857555376205724	31.211995918242962
40-44	31.235027828660154	18.87982708156672	18.771983175790417	31.113161913982708
45-49	31.355709426441848	18.671886384397514	18.640001004484514	31.332403184676128
50-54	31.15977382299292	18.74817722052128	18.823012184084703	31.269036772401094
55-59	31.258935286327134	18.842933919166104	18.767126721089294	31.13100407341747
60-64	31.37221254716901	18.652327587801736	18.62281242546834	31.352647439560915
65-69	31.17995	18.748514999999998	18.79291	31.278624999999998
70-74	31.29136	18.83887	18.72964	31.14013
75-79	31.373326647984427	18.66451971423684	18.62117029042814	31.340983347350598
80-84	31.174078458877485	18.759799957829422	18.78911230936719	31.2770092739259
85-89	31.290758507432553	18.83400111971909	18.746684630972958	31.128555741875395
90-94	31.412252915354188	18.64335633355669	18.58656954761227	31.35782120347685
95-99	31.214685853968295	18.735963668527017	18.765728480008796	31.283621997495896
100-101	31.470064226228356	18.90347811844782	18.672141170175124	30.954316485148702
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.68951638151484
2	56.790932982950125
3	59.466772793495736
4	61.20133443760106
5	59.54172130236136
6	63.32956702092031
7	62.23197721617028
8	60.6461328961441
9	62.952640798067335
10-14	61.63009282934274
15-19	62.22142495663409
20-24	62.12968858398116
25-29	62.10360485153993
30-34	62.50817304292067
35-39	62.30549518525103
40-44	62.34818974264286
45-49	62.68811261111797
50-54	62.42881059539401
55-59	62.38993935974461
60-64	62.72485998672993
65-69	62.458575
70-74	62.431490000000004
75-79	62.71430999533503
80-84	62.45108773280339
85-89	62.41931424930796
90-94	62.77007411883103
95-99	62.49830785146418
100-101	62.42438071137706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	34.0
2	18.0
3	19.0
4	20.5
5	21.0
6	22.5
7	20.0
8	27.5
9	45.0
10	57.0
11	70.0
12	95.5
13	136.5
14	176.0
15	194.0
16	216.5
17	280.0
18	343.0
19	389.5
20	462.0
21	554.5
22	651.5
23	817.0
24	1030.5
25	1243.5
26	1514.5
27	1881.5
28	2285.5
29	2718.0
30	3322.5
31	4032.5
32	4711.5
33	5492.0
34	6194.5
35	6834.0
36	7573.5
37	8390.5
38	9358.5
39	10178.5
40	10949.5
41	11998.0
42	13317.0
43	14905.5
44	16983.5
45	19772.0
46	23246.5
47	27473.5
48	32844.5
49	39324.0
50	47208.0
51	56205.5
52	65888.0
53	76629.0
54	88224.0
55	100084.5
56	111653.0
57	123030.5
58	134519.5
59	145833.0
60	156936.5
61	168290.0
62	178511.5
63	187380.0
64	194357.0
65	198782.0
66	200738.5
67	198528.5
68	191855.0
69	181642.0
70	167518.0
71	150425.0
72	131871.5
73	111535.5
74	91266.0
75	72464.5
76	55169.0
77	40581.0
78	28968.5
79	19617.0
80	12631.0
81	7863.5
82	4582.5
83	2536.5
84	1430.5
85	844.0
86	554.5
87	380.0
88	271.0
89	209.5
90	160.5
91	131.0
92	102.5
93	89.0
94	70.5
95	50.0
96	36.0
97	26.5
98	19.0
99	15.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18475
2	0.185475
3	0.182325
4	0.175175
5	0.1721
6	0.12335
7	0.130925
8	0.165275
9	0.17884999999999998
10-14	0.11348
15-19	0.102995
20-24	0.07177499999999999
25-29	0.069255
30-34	0.106055
35-39	0.077345
40-44	0.049985
45-49	0.048235
50-54	0.066765
55-59	0.055665
60-64	5.5E-4
65-69	0.0
70-74	0.0
75-79	0.010205
80-84	0.02508
85-89	0.0017050000000000001
90-94	0.003145
95-99	0.016165
100-101	0.021374999999999998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.25928349827893543
#Duplication Level	Relative count
1	100.0
2	0.17280413527808944
3	0.0010017631030613881
4	0.0
5	0.0
6	5.008815515306941E-4
7	0.0
8	5.008815515306941E-4
9	0.0
10++	0.001502644654592082
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	292675	3.2316709	3.7325191	10-14
AAAAA	304450	3.2280393	4.1496816	1
>>END_MODULE