##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002007_ItsyBitsy_ATTCCT_L003_R2_005.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 62 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6096395 34.0 31.0 34.0 31.0 34.0 2 32.7571705 34.0 31.0 34.0 31.0 34.0 3 32.8105305 34.0 31.0 34.0 31.0 34.0 4 36.20015575 37.0 37.0 37.0 35.0 37.0 5 36.2093525 37.0 37.0 37.0 35.0 37.0 6 36.20391275 37.0 37.0 37.0 35.0 37.0 7 36.1911025 37.0 37.0 37.0 35.0 37.0 8 36.1570895 37.0 37.0 37.0 35.0 37.0 9 37.92553675 39.0 39.0 39.0 37.0 39.0 10-14 38.2143023 39.4 39.0 39.4 35.8 39.4 15-19 39.3841092 41.0 40.0 41.0 37.0 41.0 20-24 39.19786295 41.0 39.2 41.0 36.0 41.0 25-29 38.8069081 40.4 38.6 41.0 35.0 41.0 30-34 38.15289805 40.0 37.4 41.0 34.0 41.0 35-39 37.1786225 39.0 35.2 40.8 33.0 41.0 40-44 36.246995399999996 37.0 35.0 40.0 32.0 41.0 45-49 35.2765183 35.0 35.0 38.8 31.2 41.0 50-54 33.90964585 34.8 33.4 36.2 29.6 39.2 55-59 34.0707784 35.0 35.0 35.0 31.0 38.2 60-64 33.69727965 35.0 35.0 35.0 31.0 36.4 65-69 33.39877524999999 35.0 34.0 35.0 30.2 35.0 70-74 33.15780595 35.0 34.0 35.0 30.0 35.0 75-79 32.93812765 35.0 33.8 35.0 29.0 35.0 80-84 32.6761331 35.0 33.0 35.0 29.0 35.0 85-89 32.384875449999996 35.0 33.0 35.0 27.4 35.0 90-94 32.04623475 35.0 33.0 35.0 27.0 35.0 95-99 31.55486805 35.0 33.0 35.0 25.0 35.0 100-101 30.50764925 34.0 31.5 35.0 22.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7299.0 3 979.0 4 1311.0 5 1500.0 6 2003.0 7 2554.0 8 3093.0 9 3579.0 10 3860.0 11 4170.0 12 4257.0 13 4327.0 14 4334.0 15 4583.0 16 4951.0 17 5463.0 18 6151.0 19 6984.0 20 8101.0 21 9727.0 22 11873.0 23 14446.0 24 17931.0 25 21866.0 26 26798.0 27 32937.0 28 41509.0 29 51191.0 30 64041.0 31 82624.0 32 109216.0 33 154322.0 34 255264.0 35 637859.0 36 1451778.0 37 787871.0 38 131021.0 39 18153.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.21845262473802 22.72216119435268 13.561889102402985 26.497497078506317 2 31.46575376758624 24.065888950082613 19.09863315475892 25.369724127572223 3 28.724953381992798 19.62855292548804 20.899611760679964 30.746881931839205 4 30.999878354207517 19.360478533522425 19.44460435832345 30.19503875394661 5 31.38648777018529 20.600797686918146 19.836318528228098 28.17639601466847 6 29.670617441458152 19.054898428318996 17.630326248109192 33.64415788211366 7 32.86080212110672 18.67464886323314 19.07253166297181 29.39201735268833 8 28.633826011630486 19.473601939116044 19.810432167762798 32.08213988149067 9 30.706505128243673 17.84368251486865 19.18860011724563 32.26121223964205 10-14 30.883396592929817 19.20840455577252 19.115769693308003 30.79242915798966 15-19 31.076764708591643 18.802909017216443 18.921973705342356 31.198352568849558 20-24 30.97486685351206 18.853971078582514 18.976726933257076 31.194435134648344 25-29 31.122325038428045 18.967117687148843 18.896056963085158 31.014500311337954 30-34 31.266600855228305 18.725627027164766 18.711504551307016 31.296267566299917 35-39 31.108944395056064 18.807368949921283 18.853537618556512 31.23014903646614 40-44 31.2588318461529 18.87042041927084 18.765335242480795 31.105412492095468 45-49 31.340305737748803 18.664527546467824 18.620902903522943 31.374263812260434 50-54 31.16550911167955 18.739239562738668 18.807057465664183 31.288193859917595 55-59 31.276952306342576 18.82250674231422 18.74743896640049 31.15310198494271 60-64 31.38241323743226 18.621629504525846 18.61460408405443 31.381353173987463 65-69 31.172965 18.756535 18.77391 31.296590000000002 70-74 31.29679 18.829655000000002 18.712825 31.160729999999997 75-79 31.383630381569915 18.631006507301215 18.62280904929343 31.36255406183544 80-84 31.17178869281724 18.750384855393165 18.775976019538522 31.301850432251072 85-89 31.310029698834548 18.820171804074803 18.711285912178884 31.15851258491177 90-94 31.40263326104309 18.633016825370678 18.586733467513067 31.377616446073166 95-99 31.213949805898917 18.724763144550145 18.758924615503364 31.302362434047577 100-101 31.39907467527595 18.925911993605066 18.70661133460258 30.968401996516405 >>END_MODULE >>Per base GC content warn #Base %GC 1 63.715949703244334 2 56.83547789515846 3 59.471835313832 4 61.19491710815412 5 59.56288378485376 6 63.314775323571816 7 62.25281947379505 8 60.71596589312116 9 62.967717367885726 10-14 61.67582575091948 15-19 62.2751172774412 20-24 62.169301988160406 25-29 62.136825349766 30-34 62.562868421528215 35-39 62.33909343152221 40-44 62.36424433824836 45-49 62.71456955000924 50-54 62.453702971597146 55-59 62.43005429128529 60-64 62.76376641141972 65-69 62.469555 70-74 62.45752 75-79 62.74618444340535 80-84 62.47363912506832 85-89 62.46854228374631 90-94 62.780249707116255 95-99 62.516312239946494 100-101 62.36747667179235 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8.0 1 8.0 2 7.0 3 9.0 4 12.5 5 15.5 6 29.0 7 44.0 8 67.5 9 103.5 10 131.5 11 172.5 12 220.0 13 241.5 14 255.5 15 289.0 16 301.0 17 319.0 18 345.5 19 378.5 20 443.0 21 520.5 22 637.5 23 785.5 24 930.0 25 1147.5 26 1453.5 27 1833.0 28 2270.5 29 2729.0 30 3284.5 31 3905.0 32 4555.5 33 5306.0 34 6083.0 35 6829.0 36 7479.5 37 8219.0 38 9131.0 39 9984.0 40 10829.5 41 11813.0 42 13147.5 43 14837.0 44 16822.0 45 19492.5 46 22968.5 47 27315.5 48 32662.5 49 39246.5 50 47020.0 51 55752.5 52 65776.0 53 76715.5 54 87852.5 55 99269.0 56 111310.0 57 123047.0 58 134239.0 59 146168.0 60 157754.0 61 168146.0 62 178378.0 63 187517.0 64 194321.0 65 198758.0 66 200212.5 67 198270.0 68 192562.5 69 182450.0 70 168450.0 71 151484.5 72 132208.5 73 111973.0 74 91980.0 75 73040.0 76 55978.5 77 41219.0 78 28965.5 79 19509.5 80 12494.0 81 7440.0 82 4301.0 83 2377.0 84 1256.5 85 704.5 86 400.0 87 262.5 88 191.5 89 147.5 90 124.0 91 86.0 92 59.0 93 46.0 94 40.5 95 36.5 96 32.5 97 24.0 98 17.5 99 12.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1367 2 0.135 3 0.132475 4 0.11984999999999998 5 0.09865 6 0.14195 7 0.165325 8 0.20195 9 0.20950000000000002 10-14 0.14558500000000002 15-19 0.12572 20-24 0.090305 25-29 0.09249 30-34 0.123745 35-39 0.094585 40-44 0.081055 45-49 0.06795 50-54 0.08538000000000001 55-59 0.07696499999999999 60-64 0.005985 65-69 0.0 70-74 0.0 75-79 0.029985 80-84 0.043625 85-89 0.014595 90-94 0.007255 95-99 0.048215 100-101 0.045899999999999996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.285678611561982 #Duplication Level Relative count 1 100.0 2 0.1657876415581033 3 0.005008690077284088 4 0.0 5 0.0 6 0.0010017380154568175 7 0.0 8 0.0 9 0.0 10++ 0.002003476030913635 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 282255 3.1291857 3.6843896 10-14 AAAAA 288025 3.0710878 3.8701086 1 >>END_MODULE