##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.632416	34.0	31.0	34.0	31.0	34.0
2	32.7768375	34.0	31.0	34.0	31.0	34.0
3	32.8419985	34.0	31.0	34.0	31.0	34.0
4	36.224264	37.0	37.0	37.0	35.0	37.0
5	36.23641775	37.0	37.0	37.0	35.0	37.0
6	36.20742675	37.0	37.0	37.0	35.0	37.0
7	36.1990185	37.0	37.0	37.0	35.0	37.0
8	36.1635635	37.0	37.0	37.0	35.0	37.0
9	37.93240525	39.0	39.0	39.0	37.0	39.0
10-14	38.209180149999995	39.4	39.0	39.4	35.8	39.4
15-19	39.3814516	41.0	40.0	41.0	37.0	41.0
20-24	39.17468755	41.0	39.2	41.0	36.0	41.0
25-29	38.77529955	40.2	38.4	41.0	35.0	41.0
30-34	38.0985643	40.0	37.2	41.0	33.8	41.0
35-39	37.11887295	38.8	35.2	40.8	32.8	41.0
40-44	36.1640909	37.0	35.0	40.0	32.0	41.0
45-49	35.17902600000001	35.0	35.0	38.4	31.0	41.0
50-54	33.8198486	34.8	33.4	36.0	29.4	39.2
55-59	33.9910544	35.0	35.0	35.0	31.0	38.2
60-64	33.6021272	35.0	34.6	35.0	30.8	36.4
65-69	33.26476889999999	35.0	34.0	35.0	30.0	35.0
70-74	32.99852675	35.0	34.0	35.0	29.0	35.0
75-79	32.751606499999994	35.0	33.2	35.0	29.0	35.0
80-84	32.44451925	35.0	33.0	35.0	27.8	35.0
85-89	32.111409249999994	35.0	33.0	35.0	27.0	35.0
90-94	31.69666305	35.0	33.0	35.0	25.2	35.0
95-99	31.128409499999997	35.0	33.0	35.0	23.8	35.0
100-101	29.938258125	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7559.0
3	1080.0
4	1598.0
5	1839.0
6	2394.0
7	2870.0
8	3598.0
9	4129.0
10	4217.0
11	4512.0
12	4623.0
13	4888.0
14	4939.0
15	5007.0
16	5391.0
17	6148.0
18	6911.0
19	7910.0
20	9254.0
21	10791.0
22	12969.0
23	15500.0
24	19122.0
25	23161.0
26	28231.0
27	34836.0
28	42442.0
29	53256.0
30	66500.0
31	85382.0
32	113658.0
33	162405.0
34	268964.0
35	661272.0
36	1417401.0
37	751262.0
38	126573.0
39	17345.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.23418610583912	22.710340996344115	13.55080371611836	26.504669181698397
2	31.375141726548478	24.04338021259608	19.146236769060188	25.43524129179525
3	28.695453272547134	19.62219425440762	20.889129582270463	30.793222890774786
4	31.00401111972776	19.315960072479925	19.39208351256875	30.28794529522357
5	31.410824244899267	20.606823131866374	19.84121264533487	28.141139977899492
6	29.71774156183687	19.010538954008627	17.584558194655965	33.68716128949853
7	32.91733838041257	18.706747729866372	19.01804885233386	29.357865037387192
8	28.62156652059386	19.47947028895152	19.84612238843071	32.0528408020239
9	30.72585936460719	17.82116678328537	19.171689037165415	32.281284814942026
10-14	30.865347711105617	19.222772099991463	19.1173247549397	30.794555433963218
15-19	31.096284987934585	18.772078440229638	18.935743783047112	31.195892788788665
20-24	30.981071983995882	18.83228938416959	18.98162731550367	31.20501131633086
25-29	31.133693911692	18.95132772681295	18.890217642182662	31.02476071931239
30-34	31.295227377175074	18.718734950073042	18.712201842486525	31.273835830265362
35-39	31.105447097699944	18.82826182667919	18.832245196211474	31.234045879409393
40-44	31.247676107111204	18.866650291316283	18.76038731022761	31.125286291344906
45-49	31.37263221178622	18.634252333390506	18.63299150098895	31.360123953834325
50-54	31.181432384184475	18.73470350005387	18.786387635721525	31.29747648004013
55-59	31.281009349606965	18.808681501946197	18.746178643278622	31.16413050516822
60-64	31.371988994098682	18.62497336753689	18.616507981938575	31.38652965642585
65-69	31.16492551440317	18.74506436858368	18.779304413095737	31.310705703917414
70-74	31.311410753962583	18.812654506574464	18.726259325144586	31.149675414318367
75-79	31.404155393067462	18.634045391013885	18.591943505651646	31.369855710267007
80-84	31.208413916121664	18.724851254190092	18.77515062171869	31.291584207969553
85-89	31.330585076313312	18.800491271902878	18.7046361448087	31.164287506975114
90-94	31.429702485520146	18.615887309926055	18.557759383184944	31.396650821368855
95-99	31.230866042373812	18.725303790855442	18.742902892230717	31.30092727454003
100-101	31.471532705416223	18.909993280980924	18.65940250625789	30.959071507344966
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.73885528753752
2	56.810383018343735
3	59.48867616332192
4	61.29195641495132
5	59.55196422279876
6	63.40490285133541
7	62.27520341779977
8	60.67440732261776
9	63.007144179549215
10-14	61.65990314506884
15-19	62.29217777672324
20-24	62.18608330032674
25-29	62.15845463100439
30-34	62.569063207440436
35-39	62.33949297710934
40-44	62.37296239845611
45-49	62.73275616562054
50-54	62.4789088642246
55-59	62.44513985477518
60-64	62.75851865052453
65-69	62.47563121832058
70-74	62.46108616828096
75-79	62.77401110333447
80-84	62.49999812409121
85-89	62.49487258328842
90-94	62.826353306889004
95-99	62.53179331691384
100-101	62.43060421276119
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	6.0
2	4.0
3	6.5
4	8.5
5	15.5
6	27.0
7	39.5
8	56.0
9	79.0
10	116.0
11	159.0
12	182.5
13	219.5
14	251.0
15	264.0
16	283.0
17	318.0
18	350.0
19	382.0
20	451.5
21	546.5
22	643.0
23	790.5
24	983.0
25	1189.0
26	1448.0
27	1800.5
28	2249.0
29	2770.5
30	3354.5
31	3907.5
32	4489.5
33	5280.5
34	6100.5
35	6840.0
36	7535.0
37	8226.0
38	9012.0
39	9861.0
40	10822.0
41	11808.5
42	13064.5
43	14821.5
44	16866.0
45	19642.5
46	23020.5
47	27285.5
48	32679.5
49	39047.5
50	46995.0
51	55859.0
52	65560.5
53	76534.0
54	87884.0
55	99390.0
56	111145.0
57	122760.5
58	134417.5
59	146341.0
60	157362.5
61	167713.0
62	178238.5
63	187527.5
64	194254.0
65	198440.5
66	200150.5
67	198253.5
68	192247.0
69	182341.5
70	168395.0
71	151229.5
72	132351.0
73	112620.0
74	92088.0
75	72733.5
76	55617.5
77	40793.5
78	29014.0
79	19784.5
80	12808.5
81	7857.5
82	4624.0
83	2618.0
84	1508.0
85	905.0
86	573.0
87	399.0
88	310.0
89	245.0
90	181.0
91	132.0
92	110.0
93	95.0
94	73.5
95	59.5
96	49.5
97	34.5
98	28.5
99	22.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.140425
2	0.11574999999999999
3	0.103425
4	0.096475
5	0.0928
6	0.177475
7	0.1931
8	0.23240000000000002
9	0.208975
10-14	0.14447500000000002
15-19	0.11324499999999998
20-24	0.09571
25-29	0.09014
30-34	0.12409999999999999
35-39	0.08459
40-44	0.07338499999999999
45-49	0.06602
50-54	0.085395
55-59	0.08457
60-64	0.004555
65-69	1.3000000000000002E-4
70-74	2.0999999999999998E-4
75-79	0.028229999999999998
80-84	0.048445
85-89	0.010565000000000001
90-94	0.0050349999999999995
95-99	0.051715
100-101	0.0412125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2508093848674907
#Duplication Level	Relative count
1	100.0
2	0.1657901327322815
3	0.002504382669671926
4	5.008765339343851E-4
5	0.0
6	0.0010017530678687703
7	0.0
8	5.008765339343851E-4
9	0.0
10++	0.005008765339343852
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	286395	3.1801898	3.7765179	10-14
AAAAA	287445	3.0734031	3.94816	1
>>END_MODULE