##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6163745	34.0	31.0	34.0	31.0	34.0
2	32.793883	34.0	31.0	34.0	31.0	34.0
3	32.83123825	34.0	31.0	34.0	31.0	34.0
4	36.2297325	37.0	37.0	37.0	35.0	37.0
5	36.2379055	37.0	37.0	37.0	35.0	37.0
6	36.185	37.0	37.0	37.0	35.0	37.0
7	36.18862975	37.0	37.0	37.0	35.0	37.0
8	36.1528835	37.0	37.0	37.0	35.0	37.0
9	37.899734	39.0	39.0	39.0	37.0	39.0
10-14	38.21173915	39.4	39.2	39.4	35.8	39.4
15-19	39.392517	41.0	40.0	41.0	37.0	41.0
20-24	39.18580805	41.0	39.2	41.0	36.0	41.0
25-29	38.79465595	40.4	38.6	41.0	35.0	41.0
30-34	38.1124333	40.0	37.2	41.0	33.8	41.0
35-39	37.14252885	39.0	35.2	40.8	33.0	41.0
40-44	36.17431045000001	37.0	35.0	40.0	32.0	41.0
45-49	35.17557715	35.0	35.0	38.4	31.0	41.0
50-54	33.79324415000001	34.8	33.2	36.0	28.8	39.2
55-59	33.9900822	35.0	35.0	35.0	31.0	38.2
60-64	33.61185210000001	35.0	34.6	35.0	30.8	36.4
65-69	33.29301125	35.0	34.0	35.0	30.0	35.0
70-74	33.01644745	35.0	34.0	35.0	29.2	35.0
75-79	32.7485481	35.0	33.0	35.0	29.0	35.0
80-84	32.43846535	35.0	33.0	35.0	27.4	35.0
85-89	32.12089695	35.0	33.0	35.0	27.0	35.0
90-94	31.7071416	35.0	33.0	35.0	25.2	35.0
95-99	31.1171267	35.0	32.8	35.0	23.6	35.0
100-101	29.999420625	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8257.0
3	1030.0
4	1388.0
5	1731.0
6	2083.0
7	2747.0
8	3259.0
9	3837.0
10	4128.0
11	4421.0
12	4573.0
13	4748.0
14	4962.0
15	5151.0
16	5506.0
17	5992.0
18	6664.0
19	7852.0
20	8979.0
21	10717.0
22	13031.0
23	15879.0
24	19134.0
25	23158.0
26	28364.0
27	34396.0
28	43211.0
29	53186.0
30	67604.0
31	86475.0
32	115324.0
33	162906.0
34	269938.0
35	652353.0
36	1411290.0
37	760839.0
38	127633.0
39	17195.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.24329322846255	22.74026051097089	13.559626333347774	26.45681992721879
2	31.44153668339042	24.046993703438407	19.10337443442821	25.40809517874296
3	28.70548944624075	19.608504066029404	20.926394570386215	30.759611917343626
4	31.056764424311183	19.344938873739455	19.383122456200226	30.21517424574914
5	31.370758892149002	20.62064602284615	19.823728681216046	28.184866403788806
6	29.711726939771328	19.01989815516166	17.61272251152789	33.65565239353912
7	32.86944104876595	18.70412388237069	19.031011190452205	29.395423878411155
8	28.561110516397342	19.504709004882866	19.858319960630226	32.07586051808956
9	30.77039124639353	17.848055872079023	19.13095952010838	32.250593361419064
10-14	30.866000759054945	19.22661736649202	19.134515093804588	30.772866780648446
15-19	31.05946703508442	18.793871046643567	18.951160785255254	31.195501133016755
20-24	30.958050011766115	18.845490174406297	18.984170634632804	31.212289179194784
25-29	31.126055072091642	18.95328039297095	18.88319710337085	31.03746743156656
30-34	31.278790596521905	18.736519988011924	18.705673479188246	31.279015936277922
35-39	31.10114934210233	18.808654707238652	18.85602616205582	31.234169788603193
40-44	31.23792388864747	18.85565752993895	18.762491450518493	31.143927130895086
45-49	31.36413877575815	18.64168323659252	18.636399141154353	31.357778846494977
50-54	31.153479170321184	18.750762287244598	18.803842753497886	31.29191578893633
55-59	31.286739064211066	18.81936055520779	18.755381438568087	31.138518942013054
60-64	31.36962193634317	18.63333058831041	18.61123040377387	31.385817071572546
65-69	31.15908	18.742415	18.789905	31.3086
70-74	31.29282	18.829985	18.716565	31.16063
75-79	31.38724750814622	18.627828871760148	18.609574173913657	31.375349446179975
80-84	31.17890511449925	18.739252531514737	18.773962614768905	31.30787973921711
85-89	31.300714346002195	18.83205548681972	18.71825634453094	31.148973822647147
90-94	31.437297859324463	18.630564657641163	18.57986464496616	31.352272838068206
95-99	31.23956414757396	18.730669159264515	18.74720343168449	31.282563261477037
100-101	31.431655318634878	18.91008622136428	18.662749734442798	30.995508725558047
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.700113155681336
2	56.849631862133386
3	59.46510136358438
4	61.27193867006032
5	59.55562529593781
6	63.367379333310446
7	62.26486492717711
8	60.636971034486905
9	63.0209846078126
10-14	61.63886753970339
15-19	62.25496816810118
20-24	62.1703391909609
25-29	62.1635225036582
30-34	62.55780653279983
35-39	62.33531913070552
40-44	62.38185101954256
45-49	62.72191762225313
50-54	62.44539495925751
55-59	62.42525800622413
60-64	62.75543900791571
65-69	62.46768
70-74	62.45345
75-79	62.7625969543262
80-84	62.48678485371636
85-89	62.449688168649345
90-94	62.78957069739267
95-99	62.522127409050995
100-101	62.42716404419292
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.5
2	3.0
3	2.5
4	6.5
5	8.0
6	10.0
7	15.0
8	26.5
9	51.0
10	66.0
11	93.5
12	144.5
13	202.5
14	256.0
15	303.5
16	336.5
17	382.5
18	423.0
19	452.0
20	508.5
21	595.0
22	682.0
23	790.0
24	983.5
25	1222.5
26	1515.5
27	1880.0
28	2287.0
29	2770.0
30	3337.0
31	3985.5
32	4668.0
33	5301.5
34	5994.0
35	6784.0
36	7527.5
37	8275.0
38	9055.5
39	9903.0
40	10790.0
41	11846.5
42	13147.5
43	14802.0
44	16903.0
45	19569.0
46	23086.0
47	27331.5
48	32734.0
49	39264.0
50	46915.0
51	55912.0
52	65828.0
53	76663.5
54	88252.5
55	99671.0
56	111237.5
57	123135.5
58	134569.5
59	145893.5
60	156912.0
61	167867.5
62	178544.5
63	187349.0
64	194321.5
65	199114.5
66	200832.5
67	198652.0
68	192321.5
69	182053.0
70	168175.5
71	151148.5
72	131777.5
73	111826.5
74	91763.5
75	72762.0
76	55612.5
77	40700.0
78	28736.5
79	19399.0
80	12537.5
81	7733.0
82	4581.0
83	2623.0
84	1451.0
85	835.5
86	516.0
87	355.5
88	251.5
89	181.5
90	142.5
91	121.5
92	104.0
93	76.5
94	61.0
95	46.0
96	31.5
97	24.0
98	22.5
99	21.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.159675
2	0.11215000000000001
3	0.096025
4	0.08795
5	0.1057
6	0.22567500000000001
7	0.24085
8	0.278825
9	0.239975
10-14	0.16532999999999998
15-19	0.12063
20-24	0.115705
25-29	0.104575
30-34	0.150775
35-39	0.098065
40-44	0.08166
45-49	0.077505
50-54	0.09507499999999999
55-59	0.10803
60-64	8.35E-4
65-69	0.0
70-74	0.0
75-79	0.025735
80-84	0.057859999999999995
85-89	0.0036399999999999996
90-94	2.4999999999999998E-5
95-99	0.05608
100-101	0.045075
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2966766224814928
#Duplication Level	Relative count
1	100.0
2	0.176331503226065
3	0.00400753416422875
4	0.0030056506231715624
5	0.0
6	5.009417705285937E-4
7	5.009417705285937E-4
8	0.0010018835410571874
9	0.0
10++	0.0030056506231715624
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	283295	3.1370256	3.871813	9
AAAAA	284580	3.0341363	3.776818	1
>>END_MODULE