##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60665025	34.0	31.0	34.0	31.0	34.0
2	32.7676555	34.0	31.0	34.0	31.0	34.0
3	32.8022685	34.0	31.0	34.0	31.0	34.0
4	36.21348725	37.0	37.0	37.0	35.0	37.0
5	36.1996165	37.0	37.0	37.0	35.0	37.0
6	36.19408525	37.0	37.0	37.0	35.0	37.0
7	36.1992655	37.0	37.0	37.0	35.0	37.0
8	36.17588925	37.0	37.0	37.0	35.0	37.0
9	37.9497725	39.0	39.0	39.0	37.0	39.0
10-14	38.22684305	39.4	39.0	39.4	35.4	39.4
15-19	39.3762604	41.0	40.0	41.0	36.8	41.0
20-24	39.17150790000001	41.0	39.0	41.0	35.8	41.0
25-29	38.77387865	40.4	38.4	41.0	35.0	41.0
30-34	38.1065911	40.0	37.2	41.0	33.8	41.0
35-39	37.114329100000006	38.8	35.2	40.8	32.8	41.0
40-44	36.162062250000005	37.0	35.0	40.0	32.0	41.0
45-49	35.1821331	35.0	35.0	38.4	31.0	41.0
50-54	33.80579504999999	34.8	33.4	36.0	29.0	39.2
55-59	33.969887650000004	35.0	35.0	35.0	31.0	38.0
60-64	33.5896192	35.0	34.4	35.0	30.6	36.4
65-69	33.2377634	35.0	34.0	35.0	29.8	35.0
70-74	32.987115499999994	35.0	34.0	35.0	29.0	35.0
75-79	32.71260595	35.0	33.0	35.0	29.0	35.0
80-84	32.42020545	35.0	33.0	35.0	27.4	35.0
85-89	32.0998765	35.0	33.0	35.0	27.0	35.0
90-94	31.703846050000003	35.0	33.0	35.0	25.2	35.0
95-99	31.14251625	35.0	33.0	35.0	23.8	35.0
100-101	29.99293525	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5667.0
3	1067.0
4	1462.0
5	1853.0
6	2416.0
7	2963.0
8	3414.0
9	4113.0
10	4364.0
11	4743.0
12	4933.0
13	4872.0
14	4913.0
15	5367.0
16	5543.0
17	6117.0
18	7129.0
19	8023.0
20	9290.0
21	11024.0
22	13157.0
23	16089.0
24	19428.0
25	23769.0
26	29231.0
27	35312.0
28	43777.0
29	54805.0
30	68355.0
31	87275.0
32	115792.0
33	164511.0
34	268948.0
35	644758.0
36	1408314.0
37	761737.0
38	128051.0
39	17351.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.187900083966	22.73335613149566	13.631381753893004	26.447362030645337
2	31.388289189747926	24.07510207677495	19.11865333240563	25.417955401071502
3	28.71303086469498	19.600780639577867	20.92943460691974	30.756753888807413
4	30.999663682516292	19.349215659582647	19.407095297961273	30.244025359939787
5	31.38827524295499	20.590314735919797	19.866711681735786	28.154698339389427
6	29.71721585169151	19.036742715885232	17.588079692986362	33.65796173943689
7	32.91376114175707	18.687159613923487	19.050480092225772	29.34859915209367
8	28.61561863332828	19.44538325079348	19.84005743992766	32.09894067595059
9	30.770994565792858	17.84975214518387	19.14791411704835	32.23133917197492
10-14	30.863054117081152	19.21669857725277	19.129656967580015	30.790590338086066
15-19	31.04963141683629	18.79682832723043	18.969001732940463	31.184538522992817
20-24	30.97282922888198	18.854794469291637	18.991241846209995	31.181134455616384
25-29	31.122233922810565	18.96800810830264	18.90564424477026	31.004113724116532
30-34	31.277317696979363	18.740294115263108	18.70780232301805	31.27458586473948
35-39	31.09030793706148	18.820683515617038	18.8656254542337	31.223383093087786
40-44	31.229979876407583	18.886712952346326	18.78550731233881	31.097799858907283
45-49	31.356303447897826	18.643088887698568	18.652636470797173	31.347971193606426
50-54	31.157916575532823	18.74798027977811	18.805879134540163	31.28822401014891
55-59	31.2643640327621	18.840568690640502	18.759566851003836	31.13550042559356
60-64	31.37816800439005	18.64857979652271	18.605274309335982	31.36797788975126
65-69	31.161260000000002	18.749695	18.79541	31.293635
70-74	31.29342	18.8371	18.72372	31.14576
75-79	31.413455139176566	18.61980614096792	18.609835007748632	31.35690371210688
80-84	31.200321465337833	18.74183927883342	18.76831966097109	31.289519594857655
85-89	31.335307278832776	18.82123882955502	18.711656303677803	31.1317975879344
90-94	31.427125141700113	18.634799907839927	18.575894860715888	31.362180089744072
95-99	31.234433660155936	18.726386534769407	18.76649901374111	31.272680791333546
100-101	31.463160480646195	18.902424921691527	18.666014903457576	30.968399694204706
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.635262114611336
2	56.80624459081942
3	59.469784753502395
4	61.243689042456076
5	59.542973582344416
6	63.375177591128406
7	62.26236029385074
8	60.71455930927886
9	63.00233373776778
10-14	61.65364445516721
15-19	62.23416993982911
20-24	62.15396368449837
25-29	62.126347646927094
30-34	62.551903561718845
35-39	62.313691030149265
40-44	62.32777973531487
45-49	62.704274641504256
50-54	62.44614058568173
55-59	62.39986445835566
60-64	62.746145894141314
65-69	62.454895
70-74	62.43918000000001
75-79	62.77035885128345
80-84	62.48984106019548
85-89	62.46710486676717
90-94	62.78930523144418
95-99	62.50711445148948
100-101	62.4315601748509
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	9.5
2	8.5
3	8.0
4	5.5
5	9.5
6	11.5
7	16.0
8	21.0
9	30.0
10	53.0
11	73.0
12	97.0
13	113.0
14	119.5
15	148.0
16	175.5
17	191.5
18	240.5
19	298.0
20	333.0
21	411.5
22	546.5
23	695.0
24	877.0
25	1113.0
26	1427.0
27	1820.5
28	2194.0
29	2696.5
30	3266.5
31	3857.5
32	4663.0
33	5389.5
34	6062.0
35	6772.5
36	7505.0
37	8303.5
38	9129.0
39	9939.5
40	10741.5
41	11775.0
42	13043.5
43	14805.0
44	17038.0
45	19673.5
46	23131.5
47	27538.5
48	32750.5
49	39221.5
50	47165.0
51	56146.5
52	66058.0
53	76701.0
54	87804.5
55	99315.5
56	111419.5
57	123500.0
58	134968.0
59	146522.5
60	157901.0
61	168845.5
62	178774.5
63	187407.5
64	195017.5
65	199596.0
66	200646.5
67	198418.0
68	192165.0
69	181654.0
70	167380.5
71	150752.0
72	131839.0
73	111594.0
74	91632.5
75	72577.0
76	55233.0
77	40492.0
78	28630.0
79	19392.0
80	12567.0
81	7775.0
82	4655.0
83	2620.5
84	1470.0
85	882.5
86	550.0
87	381.5
88	268.5
89	205.0
90	165.0
91	129.5
92	109.0
93	83.5
94	62.0
95	47.5
96	38.5
97	28.5
98	22.0
99	22.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13815
2	0.11195000000000001
3	0.10755
4	0.0944
5	0.10752500000000001
6	0.1217
7	0.136375
8	0.13915
9	0.128025
10-14	0.088015
15-19	0.057155
20-24	0.045715000000000006
25-29	0.038265
30-34	0.06707
35-39	0.03769
40-44	0.030275
45-49	0.027055
50-54	0.032565
55-59	0.04548
60-64	0.0011250000000000001
65-69	0.0
70-74	0.0
75-79	0.011365
80-84	0.020325
85-89	0.002305
90-94	7.999999999999999E-5
95-99	0.018645000000000002
100-101	0.014812500000000001
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1804350433843676
#Duplication Level	Relative count
1	100.0
2	0.14270621047413512
3	0.0015021706365698434
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007235455232812E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	286445	3.1614394	3.6265433	10-14
AAAAA	291790	3.1019845	4.149023	1
>>END_MODULE