##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6397145	34.0	31.0	34.0	31.0	34.0
2	32.78089875	34.0	31.0	34.0	31.0	34.0
3	32.83527	34.0	31.0	34.0	31.0	34.0
4	36.1970275	37.0	37.0	37.0	35.0	37.0
5	36.21104175	37.0	37.0	37.0	35.0	37.0
6	36.2056265	37.0	37.0	37.0	35.0	37.0
7	36.21005625	37.0	37.0	37.0	35.0	37.0
8	36.18853425	37.0	37.0	37.0	35.0	37.0
9	37.96677425	39.0	39.0	39.0	37.0	39.0
10-14	38.2516794	39.4	39.2	39.4	37.0	39.4
15-19	39.43967915	41.0	40.0	41.0	37.0	41.0
20-24	39.2372188	41.0	39.4	41.0	36.2	41.0
25-29	38.84614875	40.6	38.6	41.0	35.0	41.0
30-34	38.183950349999996	40.0	37.4	41.0	34.2	41.0
35-39	37.2159596	39.0	35.2	41.0	33.0	41.0
40-44	36.2273053	37.0	35.0	40.0	32.0	41.0
45-49	35.24305735	35.0	35.0	38.8	31.2	41.0
50-54	33.852676	34.8	33.4	36.2	29.4	39.2
55-59	34.04211795	35.0	35.0	35.0	31.0	38.2
60-64	33.678235799999996	35.0	34.8	35.0	31.0	36.4
65-69	33.3505718	35.0	34.0	35.0	30.0	35.2
70-74	33.07761905	35.0	34.0	35.0	29.4	35.0
75-79	32.816053749999995	35.0	33.4	35.0	29.0	35.0
80-84	32.49601800000001	35.0	33.0	35.0	27.8	35.0
85-89	32.191839550000005	35.0	33.0	35.0	27.0	35.0
90-94	31.816826150000004	35.0	33.0	35.0	25.8	35.0
95-99	31.229689450000002	35.0	33.0	35.0	23.8	35.0
100-101	30.170766999999998	34.0	31.5	35.0	19.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7653.0
3	969.0
4	1304.0
5	1627.0
6	2174.0
7	2598.0
8	3277.0
9	3695.0
10	4035.0
11	4307.0
12	4449.0
13	4512.0
14	4626.0
15	4805.0
16	5292.0
17	5757.0
18	6605.0
19	7510.0
20	8772.0
21	10509.0
22	12533.0
23	15158.0
24	18264.0
25	22411.0
26	27498.0
27	33975.0
28	42112.0
29	51961.0
30	66081.0
31	85172.0
32	113571.0
33	160721.0
34	264556.0
35	636143.0
36	1414283.0
37	788268.0
38	133992.0
39	18756.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.15979198933936	22.744757448273596	13.609372858332392	26.486077704054644
2	31.39230618726202	24.098147631799076	19.149856164076418	25.359690016862483
3	28.65053655828616	19.615108956265935	20.98267687184152	30.751677613606386
4	30.99546598915755	19.36414136214701	19.40987974012786	30.23051290856758
5	31.369578292804945	20.645017288790054	19.841438635755015	28.14396578264999
6	29.685835942391986	19.0467877269881	17.62627426424546	33.64110206637445
7	32.889471752611925	18.675154097728207	19.090331768870318	29.34504238078955
8	28.613312853869406	19.542308027569124	19.815259004100902	32.02912011446056
9	30.745844998681193	17.886162570007745	19.167273886795797	32.20071854451527
10-14	30.852273757250163	19.232610496002263	19.140980971725646	30.774134775021928
15-19	31.029225943997684	18.823890232104322	18.961350885675394	31.1855329382226
20-24	30.951571685549396	18.852625060714672	19.015820294378962	31.179982959356977
25-29	31.110785090578446	18.971180941328914	18.918041373606787	30.999992594485853
30-34	31.250245285905255	18.74611547219439	18.7394477002394	31.264191541660956
35-39	31.09879924622243	18.83179388873304	18.863850152429887	31.205556712614644
40-44	31.210960531535186	18.87323262910018	18.805070542730917	31.11073629663371
45-49	31.350546865880908	18.65120675126089	18.654438810244066	31.343807572614136
50-54	31.12807450797791	18.76459291091153	18.82543480239243	31.281897778718125
55-59	31.253403345050607	18.85003132040805	18.77532975758049	31.12123557696085
60-64	31.365956450620462	18.645753758992285	18.62370336429022	31.36458642609703
65-69	31.149015000000002	18.76538	18.794635	31.29097
70-74	31.27731380760209	18.859585547384167	18.73851390686344	31.1245867381503
75-79	31.3658633804039	18.658264964687696	18.61865036805166	31.357221286856742
80-84	31.18340810541766	18.75062682448129	18.788647715312614	31.277317354788437
85-89	31.294735907856197	18.843042275493865	18.73513782443526	31.127083992214676
90-94	31.411838139696474	18.637907342545184	18.590499500445052	31.35975501731329
95-99	31.217528913525094	18.740183741502456	18.76664303003569	31.275644314936756
100-101	31.41020752165023	18.92781439251201	18.694502255354827	30.96747583048294
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.645869693394005
2	56.75199620412451
3	59.402214171892545
4	61.22597889772513
5	59.51354407545493
6	63.32693800876644
7	62.234514133401476
8	60.64243296832997
9	62.946563543196454
10-14	61.626408532272094
15-19	62.21475888222028
20-24	62.13155464490637
25-29	62.1107776850643
30-34	62.514436827566215
35-39	62.30435595883708
40-44	62.3216968281689
45-49	62.69435443849505
50-54	62.40997228669604
55-59	62.374638922011464
60-64	62.730542876717486
65-69	62.439985
70-74	62.4019005457524
75-79	62.72308466726064
80-84	62.46072546020609
85-89	62.42181990007087
90-94	62.77159315700976
95-99	62.49317322846185
100-101	62.37768335213316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	3.5
2	3.0
3	3.0
4	6.0
5	14.5
6	24.0
7	33.5
8	48.0
9	70.0
10	98.0
11	120.0
12	142.5
13	166.0
14	204.5
15	252.5
16	274.5
17	276.0
18	305.0
19	359.0
20	423.5
21	509.0
22	635.5
23	767.0
24	958.0
25	1231.5
26	1537.0
27	1899.5
28	2285.5
29	2781.5
30	3336.5
31	3968.0
32	4670.5
33	5373.0
34	6227.0
35	6931.0
36	7599.0
37	8418.0
38	9236.0
39	10046.5
40	10979.0
41	12084.5
42	13238.5
43	14918.0
44	17103.0
45	19742.0
46	23125.5
47	27414.5
48	33003.5
49	39858.0
50	47821.5
51	56563.0
52	66019.0
53	76647.5
54	87846.0
55	99439.0
56	111560.5
57	123498.0
58	135210.0
59	146706.0
60	158028.0
61	168328.0
62	178041.5
63	187615.5
64	194837.0
65	198992.0
66	200351.5
67	197845.0
68	191698.0
69	181590.0
70	167325.5
71	150266.0
72	131272.5
73	111212.0
74	91122.0
75	72116.0
76	54918.5
77	40389.0
78	28594.0
79	19409.5
80	12607.0
81	7782.5
82	4558.0
83	2556.0
84	1456.5
85	868.0
86	550.5
87	372.0
88	294.5
89	237.5
90	170.0
91	131.0
92	100.0
93	81.5
94	69.5
95	64.5
96	47.5
97	25.5
98	22.0
99	20.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23107499999999997
2	0.20747500000000002
3	0.204225
4	0.19322499999999998
5	0.202675
6	0.1875
7	0.205375
8	0.19269999999999998
9	0.194075
10-14	0.15227000000000002
15-19	0.113235
20-24	0.08593
25-29	0.07446
30-34	0.11656
35-39	0.08193
40-44	0.06908
45-49	0.063705
50-54	0.060634999999999994
55-59	0.089105
60-64	0.0017900000000000001
65-69	0.0
70-74	0.001355
75-79	0.024225
80-84	0.041795
85-89	0.004125
90-94	0.005995
95-99	0.035105
100-101	0.021275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2617356469965307
#Duplication Level	Relative count
1	100.0
2	0.15174430833642163
3	0.003505644086980038
4	0.0015024188944200163
5	0.0015024188944200163
6	0.0
7	5.008062981400054E-4
8	0.0
9	0.0
10++	0.002504031490700027
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	287570	3.175985	3.6341226	10-14
AAAAA	290225	3.0826027	3.8277516	1
>>END_MODULE