##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_011.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3956744	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60482103466891	34.0	31.0	34.0	31.0	34.0
2	32.85227070540829	34.0	31.0	34.0	31.0	34.0
3	32.90509621041948	34.0	31.0	34.0	31.0	34.0
4	36.35299579654382	37.0	37.0	37.0	35.0	37.0
5	36.261785953298975	37.0	37.0	37.0	35.0	37.0
6	36.23383949024754	37.0	37.0	37.0	35.0	37.0
7	36.23035455414856	37.0	37.0	37.0	35.0	37.0
8	36.20616446249745	37.0	37.0	37.0	35.0	37.0
9	37.98886508705137	39.0	38.0	39.0	35.0	39.0
10-14	38.23311768464172	39.4	38.2	39.4	35.2	39.4
15-19	39.34721836944719	41.0	39.0	41.0	36.0	41.0
20-24	38.9653538869333	40.2	38.8	41.0	35.2	41.0
25-29	38.52861640783432	40.0	38.0	41.0	34.4	41.0
30-34	37.302348243909634	39.4	36.2	41.0	31.8	41.0
35-39	36.6617467291288	38.4	35.0	40.2	31.2	41.0
40-44	35.55670935496458	36.2	34.8	39.6	30.2	41.0
45-49	35.15788628225632	35.0	34.8	38.4	30.8	41.0
50-54	33.9806588447471	35.0	33.6	36.2	28.4	39.6
55-59	33.70356121093505	35.0	33.8	35.0	29.8	38.0
60-64	33.10402623975673	35.0	33.0	35.0	28.0	36.0
65-69	32.85339991669919	35.0	33.0	35.0	28.6	35.0
70-74	32.416659657536606	35.0	33.0	35.0	26.6	35.0
75-79	32.09293504962666	34.4	32.4	35.0	26.6	35.0
80-84	32.239834823784406	35.0	33.0	35.0	27.0	35.0
85-89	31.66453275723676	35.0	32.6	35.0	24.8	35.0
90-94	31.461783880887925	35.0	32.8	35.0	24.0	35.0
95-99	31.067634752210402	35.0	32.6	35.0	23.8	35.0
100-101	29.596597732883403	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32.0
3	9.0
4	31.0
5	118.0
6	293.0
7	926.0
8	2162.0
9	3487.0
10	4460.0
11	5014.0
12	5218.0
13	5425.0
14	5698.0
15	6198.0
16	6818.0
17	7436.0
18	7969.0
19	8709.0
20	9423.0
21	11012.0
22	13021.0
23	16149.0
24	20684.0
25	26578.0
26	34044.0
27	43008.0
28	53671.0
29	67753.0
30	84846.0
31	109025.0
32	145264.0
33	210032.0
34	349683.0
35	750078.0
36	1283976.0
37	563389.0
38	87248.0
39	7848.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.26946942103929	23.263255626780033	13.896179434115504	25.571095518065174
2	30.880112536974845	24.17833956404559	19.901919355914863	25.0396285430647
3	28.236929151848084	19.916426233286764	21.632812231471128	30.213832383394024
4	30.643352210807674	19.896738328282044	20.03235488573433	29.42755457517595
5	31.19201040047069	20.617027535771836	20.073398733908487	28.117563329848988
6	29.656126350352714	19.10285325510066	17.821901037823018	33.419119356723606
7	32.72503856706423	18.881004179193802	19.207307826839443	29.186649426902523
8	28.508010626919507	19.507200870210458	20.016078876975616	31.96870962589442
9	30.667740950640223	17.865067843661354	19.27309424112351	32.19409696457491
10-14	30.838623878623434	19.228653660686664	19.200155481375596	30.732566979314306
15-19	31.031989939202536	18.798724405723494	18.98579235856553	31.18349329650844
20-24	31.081755401013034	18.850148121968715	18.94934091182962	31.11875556518863
25-29	31.109175590871303	18.996390351916297	18.944882441408236	30.949551615804165
30-34	31.717555887176385	18.700826994823274	18.615078392328254	30.966538725672088
35-39	31.210524676608486	18.83505440063842	18.850682235110984	31.103738687642107
40-44	31.34597481557751	18.88532926709424	18.755067801881246	31.013628115447005
45-49	31.4473061689106	18.720478251815127	18.615204824977305	31.21701075429697
50-54	31.292315095442113	18.828238571916707	18.8127510902904	31.066695242350782
55-59	31.233535343426006	18.865235536489322	18.77892695817484	31.122302161909836
60-64	31.426814837173477	18.69011730628587	18.603189867414546	31.27987798912611
65-69	31.21789461795878	18.79666007467331	18.774790119714496	31.210655187653412
70-74	31.341163340362684	18.87217874090414	18.7251234853708	31.061534433362382
75-79	31.38183314361505	18.655055773130634	18.61534635548825	31.347764727766066
80-84	31.196076349017872	18.739928114519785	18.782483971040183	31.28151156542216
85-89	31.41652956722143	18.88974879027076	18.717752223629812	30.975969418877998
90-94	31.422176296353964	18.626254823522157	18.596650164023767	31.354918716100112
95-99	31.23828341899861	18.699237416024904	18.771949910518394	31.290529254458093
100-101	31.47756084295572	18.881117909068667	18.705544255579838	30.93577699239577
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.84056493910446
2	55.91974108003954
3	58.450761535242115
4	60.070906785983624
5	59.30957373031968
6	63.07524570707632
7	61.91168799396676
8	60.476720252813934
9	62.86183791521513
10-14	61.571190857937744
15-19	62.21548323571098
20-24	62.20051096620166
25-29	62.05872720667547
30-34	62.684094612848476
35-39	62.314263364250586
40-44	62.35960293102452
45-49	62.664316923207565
50-54	62.359010337792895
55-59	62.35583750533584
60-64	62.70669282629958
65-69	62.4285498056122
70-74	62.40269777372506
75-79	62.72959787138112
80-84	62.477587914440036
85-89	62.39249898609942
90-94	62.777095012454076
95-99	62.5288126734567
100-101	62.41333783535149
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.0
2	2.0
3	1.5
4	2.5
5	3.0
6	1.5
7	3.0
8	6.5
9	11.5
10	16.5
11	21.0
12	26.0
13	35.5
14	52.5
15	60.0
16	77.0
17	107.0
18	136.5
19	208.5
20	291.0
21	367.0
22	484.0
23	634.5
24	829.5
25	1060.0
26	1341.5
27	1682.5
28	2146.0
29	2678.5
30	3197.0
31	3854.0
32	4523.0
33	5235.5
34	5982.0
35	6750.5
36	7517.0
37	8300.0
38	9245.0
39	10044.0
40	10816.5
41	11854.0
42	13206.0
43	14780.5
44	16764.0
45	19463.0
46	22884.5
47	27363.0
48	33192.0
49	39688.5
50	47130.5
51	56141.5
52	65898.0
53	76519.5
54	87784.5
55	99566.5
56	111300.0
57	122682.0
58	134095.0
59	145244.5
60	156390.0
61	167185.5
62	177060.0
63	185863.5
64	192942.5
65	197335.0
66	198490.0
67	196353.0
68	190367.5
69	179679.5
70	165564.5
71	148519.0
72	129222.0
73	109688.0
74	89873.0
75	70972.0
76	54056.5
77	39390.5
78	27750.5
79	18541.5
80	11762.5
81	7145.0
82	4078.0
83	2228.0
84	1164.0
85	650.0
86	386.0
87	241.5
88	159.5
89	115.5
90	91.0
91	65.5
92	43.0
93	22.0
94	16.0
95	11.0
96	5.0
97	2.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0178682270068521
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	1.2131186652459698E-4
25-29	0.0017741860479222311
30-34	0.05460550391938422
35-39	0.024808276704280086
40-44	0.009947573055016954
45-49	0.0
50-54	0.0
55-59	2.7800636078553477E-4
60-64	0.026056777997262394
65-69	0.015962619770194888
70-74	0.0
75-79	0.0
80-84	0.0015618902815041863
85-89	0.018570824900473723
90-94	0.0154167163708342
95-99	0.0016427648591872508
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3956744.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.08849513276770336
#Duplication Level	Relative count
1	100.0
2	0.08156770119198935
3	0.001501246034208393
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	288015	3.1926196	3.6389964	10-14
AAAAA	290620	3.094158	3.8727043	1
>>END_MODULE