##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_010.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77360775	34.0	31.0	34.0	31.0	34.0
2	33.00800425	34.0	31.0	34.0	31.0	34.0
3	33.06016775	34.0	31.0	34.0	31.0	34.0
4	36.4559675	37.0	37.0	37.0	35.0	37.0
5	36.38558325	37.0	37.0	37.0	35.0	37.0
6	36.36505075	37.0	37.0	37.0	35.0	37.0
7	36.35988275	37.0	37.0	37.0	35.0	37.0
8	36.3377515	37.0	37.0	37.0	35.0	37.0
9	38.14825425	39.0	39.0	39.0	37.0	39.0
10-14	38.4002494	39.4	38.6	39.4	37.0	39.4
15-19	39.5476005	41.0	40.0	41.0	37.0	41.0
20-24	39.04590889999999	40.4	38.8	41.0	35.6	41.0
25-29	37.218397200000005	40.0	36.6	41.0	27.6	41.0
30-34	36.9844378	39.4	36.0	41.0	31.0	41.0
35-39	36.1825757	38.2	35.0	40.0	29.8	41.0
40-44	33.8216524	36.0	33.6	39.4	19.4	41.0
45-49	34.26350939999999	35.0	33.6	38.4	27.6	40.8
50-54	32.9716314	35.0	33.0	36.0	23.8	39.6
55-59	33.38377455	35.0	33.4	35.0	28.6	37.8
60-64	32.98435885	35.0	33.0	35.0	27.4	36.2
65-69	32.77848660000001	35.0	33.0	35.0	28.6	35.0
70-74	32.28638575	35.0	33.0	35.0	26.2	35.0
75-79	31.780587250000004	34.4	32.4	35.0	25.8	35.0
80-84	31.368316399999998	35.0	32.4	35.0	23.4	35.0
85-89	31.4994278	35.0	32.8	35.0	24.0	35.0
90-94	31.23355555	35.0	32.8	35.0	24.0	35.0
95-99	30.663971600000004	35.0	31.8	35.0	20.8	35.0
100-101	29.168912249999998	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34.0
3	7.0
4	43.0
5	125.0
6	411.0
7	1191.0
8	3113.0
9	4856.0
10	6001.0
11	7090.0
12	7370.0
13	7118.0
14	6753.0
15	7121.0
16	7539.0
17	8488.0
18	9382.0
19	10435.0
20	11282.0
21	13175.0
22	15342.0
23	19026.0
24	23618.0
25	29661.0
26	36776.0
27	46104.0
28	57296.0
29	72930.0
30	93865.0
31	126545.0
32	183124.0
33	295779.0
34	478757.0
35	763527.0
36	1078929.0
37	480680.0
38	78654.0
39	7839.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.35108262069086	23.098891565421642	13.8129924483441	25.7370333655434
2	30.998900000000003	24.068025000000002	19.84515	25.087925
3	28.297475	19.8471	21.6021	30.253324999999997
4	30.669025	19.832825	20.0023	29.49585
5	31.17935	20.670575	20.072000000000003	28.078075000000002
6	29.728275	19.111	17.79	33.370725
7	32.750375	18.87855	19.159925	29.211150000000004
8	28.5564	19.518025	19.969875000000002	31.955699999999997
9	30.681425	17.86905	19.283925	32.1656
10-14	30.822889999999997	19.246565	19.204105	30.72644
15-19	31.033265	18.821845	18.976275	31.168615
20-24	31.077563055689527	18.883186359476756	18.927162014714018	31.112088570119695
25-29	32.193947523024576	18.684634860284433	18.602308780503442	30.519108836187552
30-34	32.293089449340364	18.63699735836145	18.43598147746147	30.633931714836717
35-39	31.554528005487676	18.743318758777352	18.778296892942453	30.923856342792515
40-44	31.56003746860117	18.818579871262177	18.807028877127248	30.81435378300941
45-49	31.604729999999996	18.765124999999998	18.595285	31.03486
50-54	31.547831965243844	18.931165190243355	18.704819171686275	30.81618367282653
55-59	31.279356065198883	18.8506820004667	18.7659716319766	31.103990302357815
60-64	31.429670820403505	18.621816720562045	18.63326728954627	31.315245169488183
65-69	31.24107756572861	18.763676397779054	18.76666731853401	31.22857871795832
70-74	31.41599	18.849705	18.712295	31.02201
75-79	31.49731	18.668789999999998	18.566505	31.267394999999997
80-84	31.295888815170613	18.683293229957073	18.751179713276738	31.269638241595576
85-89	31.358040475231537	18.799450347724292	18.705679133281272	31.136830043762902
90-94	31.467510058307624	18.584768432594608	18.595777326440363	31.351944182657405
95-99	31.33094416225989	18.659680428034704	18.71240273199939	31.296972677706016
100-101	31.6057125	18.765024999999998	18.6353625	30.993900000000004
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.088115986234264
2	56.086825
3	58.5508
4	60.164874999999995
5	59.257425
6	63.099000000000004
7	61.961524999999995
8	60.512100000000004
9	62.847025
10-14	61.549330000000005
15-19	62.201879999999996
20-24	62.189651625809226
25-29	62.713056359212125
30-34	62.92702116417708
35-39	62.478384348280194
40-44	62.37439125161058
45-49	62.63959
50-54	62.36401563807037
55-59	62.383346367556705
60-64	62.74491598989168
65-69	62.46965628368694
70-74	62.438
75-79	62.764705
80-84	62.56552705676619
85-89	62.49487051899444
90-94	62.81945424096504
95-99	62.627916839965906
100-101	62.5996125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	3.0
2	1.5
3	2.0
4	3.0
5	4.0
6	3.5
7	4.5
8	8.5
9	11.0
10	14.0
11	22.0
12	23.0
13	29.0
14	42.0
15	52.5
16	74.0
17	111.5
18	151.0
19	195.5
20	258.5
21	341.0
22	447.5
23	585.5
24	777.0
25	1031.5
26	1354.5
27	1683.0
28	2086.0
29	2579.0
30	3123.0
31	3749.5
32	4433.0
33	5197.5
34	5985.0
35	6806.5
36	7604.5
37	8424.5
38	9204.0
39	10019.5
40	11023.0
41	12152.5
42	13455.0
43	15046.5
44	17073.0
45	19695.5
46	23420.0
47	28175.5
48	33446.5
49	39786.5
50	47698.0
51	56824.0
52	66651.5
53	77406.5
54	88800.0
55	100739.5
56	112616.5
57	124055.5
58	136081.0
59	147592.5
60	158667.5
61	169768.5
62	179602.5
63	188356.0
64	195345.0
65	199763.0
66	201002.0
67	197998.0
68	191167.5
69	180499.5
70	166194.0
71	148986.5
72	129229.5
73	109078.5
74	89780.5
75	70964.5
76	54124.0
77	39933.0
78	28314.0
79	19410.0
80	12740.5
81	7955.5
82	4849.0
83	2916.5
84	1711.5
85	1062.0
86	722.5
87	498.5
88	357.0
89	256.0
90	182.5
91	137.5
92	97.0
93	57.0
94	38.0
95	28.5
96	13.0
97	3.0
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005375
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00149
25-29	0.007385000000000001
30-34	0.060134999999999994
35-39	0.023254999999999998
40-44	2.259495
45-49	0.0
50-54	4.5E-4
55-59	4.35E-4
60-64	0.048635
65-69	0.030784999999999996
70-74	0.0
75-79	0.0
80-84	0.002185
85-89	0.03862
90-94	0.03537
95-99	0.00437
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.011499482523289044
#Duplication Level	Relative count
1	100.0
2	0.007000490034302401
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	288085	3.2164655	3.8663898	95-97
AAAAA	285390	3.061113	3.8108377	1
>>END_MODULE