##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.634636	34.0	31.0	34.0	31.0	34.0
2	32.8807205	34.0	31.0	34.0	31.0	34.0
3	32.946576	34.0	31.0	34.0	31.0	34.0
4	36.39027675	37.0	37.0	37.0	35.0	37.0
5	36.3096725	37.0	37.0	37.0	35.0	37.0
6	36.278894	37.0	37.0	37.0	35.0	37.0
7	36.259497	37.0	37.0	37.0	35.0	37.0
8	36.25690775	37.0	37.0	37.0	35.0	37.0
9	38.015091	39.0	38.0	39.0	35.0	39.0
10-14	38.289557	39.4	38.2	39.4	35.4	39.4
15-19	39.39534930000001	41.0	39.0	41.0	36.0	41.0
20-24	38.701313049999996	40.2	38.6	41.0	34.0	41.0
25-29	27.215115500000003	27.6	25.4	30.8	21.6	31.4
30-34	32.256655699999996	36.4	28.2	39.2	17.0	39.8
35-39	35.166762	37.0	34.0	40.0	27.0	41.0
40-44	34.79862000000001	35.6	34.2	39.2	28.2	41.0
45-49	34.8681603	35.0	34.6	38.4	30.4	40.8
50-54	33.664306	35.0	33.6	35.8	27.4	39.4
55-59	33.496516199999995	35.0	33.2	35.0	29.4	37.4
60-64	31.62454965	35.0	31.8	35.0	22.2	35.2
65-69	32.38707395	35.0	32.8	35.0	27.0	35.0
70-74	30.8687402	34.4	30.2	35.0	20.4	35.0
75-79	31.896109850000006	34.4	32.4	35.0	26.4	35.0
80-84	31.697646699999996	35.0	33.0	35.0	25.2	35.0
85-89	30.788980600000002	34.6	31.4	35.0	20.4	35.0
90-94	30.868847699999996	34.4	31.4	35.0	23.0	35.0
95-99	30.55744205	34.2	31.0	35.0	20.4	35.0
100-101	29.093861	33.5	29.0	35.0	8.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	13.0
4	47.0
5	154.0
6	578.0
7	2206.0
8	6122.0
9	6812.0
10	5356.0
11	5632.0
12	5324.0
13	5949.0
14	6891.0
15	7753.0
16	8399.0
17	9294.0
18	9840.0
19	10622.0
20	11717.0
21	13858.0
22	17210.0
23	22403.0
24	28961.0
25	36827.0
26	46586.0
27	59154.0
28	74602.0
29	96608.0
30	127075.0
31	179007.0
32	286322.0
33	485880.0
34	752308.0
35	977307.0
36	565404.0
37	112916.0
38	14567.0
39	256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.26764776776829	23.205018058087933	13.896301267516744	25.631032906627034
2	30.9743	24.109849999999998	19.84015	25.0757
3	28.2227	19.92535	21.6458	30.206149999999997
4	30.639	19.890275	20.029725000000003	29.441
5	31.190925000000004	20.650575	20.014975	28.143525000000004
6	29.646375000000003	19.136425000000003	17.790924999999998	33.426275
7	32.743925000000004	18.8854	19.154975	29.2157
8	28.566475000000004	19.5042	19.932725	31.996599999999997
9	30.681000000000004	17.886825	19.284075	32.1481
10-14	30.81708	19.234825	19.202535	30.74556
15-19	31.034645	18.804804999999998	18.979575	31.180975
20-24	31.374947286798914	18.83164046081813	18.904401428539	30.88901082384396
25-29	41.12067090793401	16.59775845407341	16.09022314154313	26.191347496449445
30-34	32.95576666003271	18.76635542454007	18.143212279567546	30.134665635859676
35-39	31.457418183689846	18.811132421356216	18.789293630150816	30.94215576480312
40-44	31.40977130009298	18.728792383463954	18.743085341288403	31.118350975154662
45-49	31.361860000000004	18.731455	18.62326	31.283424999999998
50-54	31.293833123337183	18.789541156703695	18.77103576161351	31.14558995834561
55-59	31.257870754867454	18.835170780317874	18.768249676119655	31.138708788695013
60-64	31.594007992864288	18.70581445462327	18.483248558711377	31.21692899380107
65-69	31.250154133470758	18.767876668812157	18.822221691978072	31.159747505739016
70-74	31.46948	18.970035	18.703465	30.857020000000002
75-79	31.38901	18.637455	18.620595	31.35294
80-84	31.276606340959585	18.779881506027905	18.770496033000065	31.17301612001245
85-89	31.456601389620936	19.05731850040087	18.71628728381664	30.769792826161552
90-94	31.445802638225047	18.639873836200792	18.587257224140263	31.327066301433902
95-99	31.26657868370164	18.688554804296906	18.73823147840082	31.30663503360063
100-101	31.5552375	18.8562625	18.6775625	30.910937500000003
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.89868067439532
2	56.05
3	58.42885
4	60.08
5	59.334450000000004
6	63.072649999999996
7	61.959624999999996
8	60.563075
9	62.829100000000004
10-14	61.56264
15-19	62.21562
20-24	62.26395811064287
25-29	67.31201840438345
30-34	63.090432295892384
35-39	62.39957394849297
40-44	62.52812227524765
45-49	62.645284999999994
50-54	62.4394230816828
55-59	62.39657954356247
60-64	62.810936986665354
65-69	62.40990163920978
70-74	62.326499999999996
75-79	62.74195
80-84	62.44962246097203
85-89	62.22639421578249
90-94	62.77286893965894
95-99	62.57321371730228
100-101	62.466175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.5
3	3.5
4	3.0
5	2.0
6	4.5
7	6.5
8	6.0
9	6.5
10	7.5
11	12.5
12	20.5
13	20.5
14	24.5
15	36.0
16	56.5
17	74.0
18	108.0
19	145.5
20	195.0
21	290.0
22	396.0
23	488.5
24	629.5
25	849.0
26	1106.0
27	1443.5
28	1822.0
29	2284.5
30	2792.0
31	3383.5
32	4002.0
33	4698.0
34	5603.5
35	6361.5
36	7134.0
37	7977.5
38	8804.0
39	9640.0
40	10420.5
41	11434.5
42	12700.5
43	14198.0
44	16174.5
45	18638.5
46	21743.0
47	25817.0
48	30955.5
49	37265.0
50	44857.5
51	53604.5
52	63189.5
53	73859.5
54	85423.0
55	97305.0
56	109705.5
57	122127.0
58	134336.0
59	146566.5
60	157785.0
61	168409.5
62	178935.5
63	188784.0
64	196727.5
65	201550.0
66	203085.0
67	201065.5
68	195276.5
69	185504.5
70	171837.5
71	154294.0
72	134651.5
73	114471.0
74	93971.0
75	74260.5
76	56599.0
77	41619.5
78	29470.5
79	19983.5
80	12708.5
81	7649.0
82	4497.5
83	2545.5
84	1405.5
85	777.0
86	441.5
87	281.0
88	204.5
89	144.0
90	102.5
91	75.5
92	52.5
93	29.5
94	17.5
95	15.0
96	8.5
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0171
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00133
25-29	0.054245
30-34	0.084755
35-39	0.040255
40-44	0.650375
45-49	0.0
50-54	0.002135
55-59	0.0016500000000000002
60-64	0.04084
65-69	0.046045
70-74	0.0
75-79	0.0
80-84	0.00504
85-89	0.023815
90-94	0.060079999999999995
95-99	0.0033699999999999997
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.03449879254226107
#Duplication Level	Relative count
1	100.0
2	0.030009302883894008
3	0.0
4	0.0
5	0.0
6	5.001550480649001E-4
7	0.0
8	0.0
9	0.0
10++	5.001550480649001E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	274160	3.1527574	3.9592783	95-97
>>END_MODULE