##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.777483	34.0	31.0	34.0	31.0	34.0
2	33.00869	34.0	31.0	34.0	31.0	34.0
3	33.058162	34.0	31.0	34.0	31.0	34.0
4	36.462289	37.0	37.0	37.0	35.0	37.0
5	36.3916765	37.0	37.0	37.0	35.0	37.0
6	36.37030775	37.0	37.0	37.0	35.0	37.0
7	36.35714325	37.0	37.0	37.0	35.0	37.0
8	36.34884725	37.0	37.0	37.0	35.0	37.0
9	38.14362575	39.0	39.0	39.0	37.0	39.0
10-14	38.346744750000006	39.4	38.8	39.4	36.2	39.4
15-19	39.546490500000004	41.0	40.0	41.0	37.0	41.0
20-24	39.0045072	40.4	38.8	41.0	35.4	41.0
25-29	28.504022050000003	27.6	24.6	31.4	20.4	40.4
30-34	34.141784200000004	37.6	33.0	39.4	20.4	40.6
35-39	35.40035105	37.8	34.4	40.0	27.4	41.0
40-44	34.06443425	35.4	33.0	39.2	24.8	41.0
45-49	33.358414700000004	35.0	32.8	38.0	23.4	40.6
50-54	32.772742	35.0	32.4	35.8	23.8	39.4
55-59	32.74009185	35.0	32.8	35.0	25.8	37.4
60-64	32.6904804	35.0	33.0	35.0	26.6	35.8
65-69	32.389004850000006	35.0	33.0	35.0	27.0	35.0
70-74	31.664999799999997	35.0	32.8	35.0	23.8	35.0
75-79	31.117171050000003	34.4	31.6	35.0	23.0	35.0
80-84	31.046756799999997	35.0	32.0	35.0	21.6	35.0
85-89	31.204792749999996	35.0	32.6	35.0	24.0	35.0
90-94	30.92570115	35.0	32.2	35.0	22.8	35.0
95-99	30.198577049999994	34.6	31.0	35.0	13.4	35.0
100-101	28.55430175	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	12.0
4	51.0
5	162.0
6	535.0
7	2054.0
8	6016.0
9	7995.0
10	7758.0
11	8257.0
12	7215.0
13	7763.0
14	8262.0
15	8883.0
16	9486.0
17	10586.0
18	11751.0
19	12624.0
20	13770.0
21	15417.0
22	18417.0
23	22828.0
24	28864.0
25	35473.0
26	44395.0
27	56231.0
28	71479.0
29	91718.0
30	123259.0
31	177671.0
32	276285.0
33	443474.0
34	705979.0
35	903463.0
36	643990.0
37	189433.0
38	26564.0
39	1848.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.281000332754246	23.13744500242378	13.8276013019166	25.753953362905378
2	30.97955	24.104025	19.83065	25.085774999999998
3	28.277925	19.8431	21.633375	30.2456
4	30.675449999999998	19.792725	20.007925	29.523899999999998
5	31.2207	20.657575	20.023075000000002	28.098649999999996
6	29.6644	19.145200000000003	17.84405	33.34635
7	32.719375	18.883875	19.179850000000002	29.216900000000003
8	28.545274999999997	19.562124999999998	19.96	31.9326
9	30.691325000000003	17.910625	19.2611	32.13695
10-14	30.891955	19.27045	19.18914	30.648455000000002
15-19	31.014605	18.811465	18.99246	31.18147
20-24	31.133993432475876	18.799625312918415	18.95162318567821	31.1147580689275
25-29	37.474092660062865	17.513445462343398	17.130548102993927	27.881913774599816
30-34	32.61272751393619	18.774701707884283	18.304459407515818	30.3081113706637
35-39	31.626447148397908	18.806642007507385	18.734627205609613	30.83228363848509
40-44	31.77537774686125	18.98646117488089	18.565537957891877	30.672623120365976
45-49	31.968580000000003	18.97071	18.481144999999998	30.579565
50-54	31.74938714889687	19.056114301005742	18.610268498483297	30.584230051614092
55-59	31.382783537087654	18.909359182019386	18.71899903924928	30.98885824164368
60-64	31.3729878602037	18.61068921175015	18.587663027932667	31.428659900113487
65-69	31.250844209756636	18.820614706769305	18.75332305973833	31.17521802373573
70-74	31.503465000000002	18.90319	18.709915	30.88343
75-79	31.684755	18.693205	18.52299	31.099050000000002
80-84	31.3953418019207	18.834988953614282	18.729732896128173	31.03993634833685
85-89	31.379741560233853	18.782013371741044	18.732176447121443	31.10606862090366
90-94	31.502525757967575	18.57796163586012	18.566417686990956	31.353094919181352
95-99	31.378773946475103	18.650777102587142	18.686413367674554	31.284035583263204
100-101	31.721899999999998	18.8085125	18.557937499999998	30.91165
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.034953695659624
2	56.065325
3	58.523525
4	60.199349999999995
5	59.31935000000001
6	63.01075
7	61.936274999999995
8	60.477875000000004
9	62.828275
10-14	61.54041
15-19	62.19607499999999
20-24	62.248751501403376
25-29	65.35600643466267
30-34	62.920838884599895
35-39	62.458730786882995
40-44	62.44800086722723
45-49	62.548145
50-54	62.33361720051096
55-59	62.37164177873134
60-64	62.80164776031718
65-69	62.42606223349236
70-74	62.386894999999996
75-79	62.783805
80-84	62.435278150257545
85-89	62.485810181137516
90-94	62.85562067714893
95-99	62.662809529738304
100-101	62.63355000000001
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.5
3	1.0
4	1.0
5	1.0
6	1.5
7	5.5
8	6.0
9	8.0
10	12.5
11	13.5
12	20.0
13	26.0
14	35.0
15	51.5
16	69.0
17	100.0
18	123.0
19	172.5
20	244.0
21	310.0
22	405.0
23	544.5
24	733.5
25	939.5
26	1201.5
27	1535.5
28	1914.5
29	2436.0
30	3024.0
31	3619.5
32	4310.5
33	5000.0
34	5678.5
35	6444.5
36	7286.0
37	8147.0
38	9052.0
39	9980.0
40	10877.5
41	11812.0
42	13115.5
43	14811.5
44	16820.5
45	19470.0
46	23065.5
47	27417.0
48	32656.5
49	39318.0
50	47180.0
51	56047.0
52	65876.5
53	76537.0
54	88150.5
55	100017.5
56	112219.0
57	124648.0
58	136620.5
59	148480.5
60	160129.5
61	170899.5
62	180863.0
63	189648.5
64	196634.5
65	201306.0
66	202131.5
67	198866.5
68	192345.5
69	181504.5
70	166261.0
71	148630.0
72	129310.0
73	108930.5
74	89059.5
75	70265.0
76	53458.5
77	39583.0
78	28011.0
79	19019.0
80	12588.0
81	8046.0
82	4974.0
83	3060.0
84	1917.0
85	1191.0
86	807.0
87	580.5
88	424.5
89	322.5
90	221.5
91	148.0
92	106.5
93	72.5
94	39.0
95	21.5
96	12.0
97	9.5
98	5.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001275
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0018899999999999998
25-29	4.38038
30-34	0.086615
35-39	0.034440000000000005
40-44	0.0031399999999999996
45-49	0.0
50-54	1.7999999999999998E-4
55-59	7.500000000000001E-5
60-64	0.04857
65-69	0.05446
70-74	0.0
75-79	0.0
80-84	0.005755000000000001
85-89	0.033960000000000004
90-94	0.07751999999999999
95-99	0.00355
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.08398572242718656
#Duplication Level	Relative count
1	100.0
2	0.06604424964726367
3	5.003352246004823E-4
4	0.0
5	5.003352246004823E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	285650	3.2976496	4.045579	95-97
AAAAA	285125	3.0773077	3.6629822	1
>>END_MODULE