##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002007_ItsyBitsy_ATTCCT_L003_R1_007.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 62 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75346425 34.0 31.0 34.0 31.0 34.0 2 32.97641975 34.0 31.0 34.0 31.0 34.0 3 33.0277015 34.0 31.0 34.0 31.0 34.0 4 36.43534775 37.0 37.0 37.0 35.0 37.0 5 36.371078 37.0 37.0 37.0 35.0 37.0 6 36.3399235 37.0 37.0 37.0 35.0 37.0 7 36.34130575 37.0 37.0 37.0 35.0 37.0 8 36.32230825 37.0 37.0 37.0 35.0 37.0 9 38.1258505 39.0 39.0 39.0 37.0 39.0 10-14 38.3942244 39.4 38.6 39.4 37.0 39.4 15-19 39.562988499999996 41.0 40.0 41.0 37.0 41.0 20-24 39.3228826 41.0 39.0 41.0 36.2 41.0 25-29 38.88668715000001 40.0 38.4 41.0 35.0 41.0 30-34 37.387981149999995 39.8 36.4 41.0 32.0 41.0 35-39 37.07404775 38.6 35.0 40.6 32.8 41.0 40-44 36.21632025 36.8 35.0 40.0 32.0 41.0 45-49 35.586465149999995 35.0 35.0 38.8 32.4 41.0 50-54 34.73658375 35.0 35.0 36.6 31.4 40.0 55-59 34.02498215 35.0 34.4 35.0 31.0 38.0 60-64 33.477949100000004 35.0 34.0 35.0 30.0 36.2 65-69 33.2287381 35.0 33.6 35.0 29.8 35.0 70-74 32.626732700000005 35.0 33.0 35.0 27.6 35.0 75-79 32.4158193 34.8 32.6 35.0 28.2 35.0 80-84 32.448693000000006 35.0 33.0 35.0 27.4 35.0 85-89 32.0666971 35.0 33.0 35.0 26.2 35.0 90-94 31.875719850000003 35.0 33.0 35.0 27.0 35.0 95-99 31.479482150000003 35.0 33.0 35.0 25.0 35.0 100-101 30.319284125000003 34.0 31.5 35.0 21.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42.0 3 10.0 4 31.0 5 80.0 6 278.0 7 756.0 8 1847.0 9 3226.0 10 4142.0 11 4442.0 12 4687.0 13 4882.0 14 5070.0 15 5422.0 16 5995.0 17 6508.0 18 7235.0 19 7846.0 20 8531.0 21 9493.0 22 11462.0 23 14045.0 24 17608.0 25 22087.0 26 28558.0 27 35764.0 28 45117.0 29 56248.0 30 71808.0 31 93254.0 32 123981.0 33 178512.0 34 299026.0 35 694633.0 36 1400755.0 37 700704.0 38 113868.0 39 12033.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.31654523017598 23.1621272153076 13.846535009910257 25.674792544606163 2 30.98638380937333 24.09928739002346 19.814252399533594 25.100076401069614 3 28.27865 19.839475 21.593325 30.288549999999997 4 30.699379337276415 19.804005982122632 20.018435377925247 29.478179302675706 5 31.186000000000003 20.6499 20.038125 28.125974999999997 6 29.696424999999998 19.0653 17.787675 33.4506 7 32.735825 18.885099999999998 19.22165 29.157424999999996 8 28.562700000000003 19.5494 19.964725 31.923174999999997 9 30.678875 17.897825 19.23835 32.18495 10-14 30.81933 19.246679999999998 19.203429999999997 30.73056 15-19 31.039489999999997 18.80408 18.970205 31.186225 20-24 30.96769196714981 18.881683747952412 19.000175152075553 31.15044913282222 25-29 31.077275 19.00066 18.94519 30.976875 30-34 31.860998472778967 18.757063797497533 18.587902856575695 30.7940348731478 35-39 31.085592948465496 18.841900829732026 18.87339679034257 31.199109431459902 40-44 31.20817737077465 18.90570473575656 18.8169561458761 31.069161747592688 45-49 31.337625000000003 18.670095 18.673395 31.318885 50-54 31.131813254310543 18.777249104736114 18.82255917496672 31.268378465986622 55-59 31.280079999999998 18.884905 18.7534 31.081615000000003 60-64 31.422748739657457 18.670327056219982 18.618574663390667 31.28834954073189 65-69 31.18137683331398 18.741764719942722 18.800551180574747 31.27630726616855 70-74 31.367185 18.92305 18.727014999999998 30.98275 75-79 31.385235 18.639400000000002 18.63873 31.336635 80-84 31.209251994724735 18.792883031749593 18.782442902291987 31.215422071233682 85-89 31.33187611886914 18.901164686486123 18.742582229834152 31.024376964810585 90-94 31.392858823498234 18.638268935536658 18.622611290177478 31.346260950787634 95-99 31.23461392017876 18.71124307899848 18.762953557320404 31.291189443502354 100-101 31.441999999999997 18.893075 18.7145625 30.9503625 >>END_MODULE >>Per base GC content warn #Base %GC 1 62.99133777478214 2 56.086460210442944 3 58.5672 4 60.177558639952125 5 59.311975 6 63.147025 7 61.89325 8 60.485875 9 62.863825 10-14 61.54989 15-19 62.225715 20-24 62.118141099972036 25-29 62.05415 30-34 62.65503334592677 35-39 62.284702379925406 40-44 62.27733911836734 45-49 62.65651 50-54 62.40019172029717 55-59 62.361695 60-64 62.71109828038936 65-69 62.457684099482535 70-74 62.349935 75-79 62.72187 80-84 62.42467406595842 85-89 62.356253083679725 90-94 62.73911977428587 95-99 62.525803363681106 100-101 62.3923625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 1.5 2 0.0 3 1.0 4 2.0 5 1.5 6 4.0 7 8.0 8 11.0 9 9.5 10 9.5 11 18.0 12 26.0 13 32.0 14 54.5 15 72.5 16 89.5 17 118.0 18 159.0 19 221.5 20 283.5 21 373.5 22 496.5 23 646.5 24 831.5 25 1040.0 26 1328.0 27 1753.5 28 2209.0 29 2707.5 30 3275.0 31 3903.0 32 4585.5 33 5330.5 34 6106.5 35 6806.0 36 7596.5 37 8457.0 38 9257.0 39 10115.5 40 11003.0 41 11976.5 42 13274.0 43 14995.0 44 17131.0 45 19943.5 46 23641.0 47 28022.5 48 33521.0 49 40211.0 50 47936.0 51 56898.0 52 66666.0 53 77328.0 54 88897.5 55 100664.0 56 112231.5 57 123671.0 58 135861.5 59 147403.0 60 158194.0 61 169044.0 62 179022.0 63 187637.0 64 194698.5 65 199304.5 66 200616.5 67 198556.5 68 192342.5 69 181698.0 70 167108.0 71 149795.0 72 130514.0 73 110024.0 74 89927.5 75 71358.5 76 54548.0 77 39873.5 78 27955.5 79 18893.0 80 12176.0 81 7398.5 82 4275.5 83 2375.0 84 1274.0 85 743.5 86 460.0 87 286.0 88 197.0 89 146.0 90 95.5 91 70.0 92 63.5 93 46.5 94 30.0 95 13.5 96 10.0 97 6.0 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0026 2 0.0014 3 0.0 4 0.00205 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.001185 25-29 0.0 30-34 0.053759999999999995 35-39 0.018925 40-44 0.004045 45-49 0.0 50-54 1.55E-4 55-59 0.0 60-64 0.014285 65-69 0.01099 70-74 0.0 75-79 0.0 80-84 0.00124 85-89 0.007855 90-94 0.016895 95-99 9.249999999999999E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.12848843604074792 #Duplication Level Relative count 1 100.0 2 0.11663938406395642 3 0.002502991074333829 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10++ 5.005982148667658E-4 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 298960 3.2651985 3.765719 95-97 AAAAA 300640 3.1743145 3.840304 1 >>END_MODULE