##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7995405	34.0	31.0	34.0	31.0	34.0
2	33.019826	34.0	31.0	34.0	31.0	34.0
3	33.0724035	34.0	33.0	34.0	31.0	34.0
4	36.46187675	37.0	37.0	37.0	35.0	37.0
5	36.4039395	37.0	37.0	37.0	35.0	37.0
6	36.375534	37.0	37.0	37.0	35.0	37.0
7	36.3738465	37.0	37.0	37.0	35.0	37.0
8	36.35415575	37.0	37.0	37.0	35.0	37.0
9	38.15195175	39.0	39.0	39.0	37.0	39.0
10-14	38.42678825	39.4	38.8	39.4	37.0	39.4
15-19	39.5778101	41.0	40.0	41.0	37.0	41.0
20-24	39.331293699999996	41.0	39.0	41.0	36.2	41.0
25-29	38.901216950000006	40.0	38.4	41.0	35.0	41.0
30-34	38.1444659	40.0	37.2	41.0	33.8	41.0
35-39	37.12881585	38.6	35.2	40.6	32.8	41.0
40-44	36.1291395	36.6	35.0	40.0	31.8	41.0
45-49	35.49502345	35.0	35.0	38.8	32.0	41.0
50-54	34.61415905	35.0	35.0	36.6	31.0	40.0
55-59	33.9549421	35.0	34.0	35.0	30.8	38.0
60-64	33.4491847	35.0	34.0	35.0	30.0	36.2
65-69	33.0714019	35.0	33.0	35.0	29.2	35.0
70-74	32.771956800000005	35.0	33.0	35.0	29.0	35.0
75-79	32.173416499999995	34.4	32.6	35.0	27.0	35.0
80-84	32.3412664	35.0	33.0	35.0	27.2	35.0
85-89	32.03538565	35.0	33.0	35.0	27.0	35.0
90-94	31.62369435	35.0	33.0	35.0	25.2	35.0
95-99	31.059861849999997	35.0	32.6	35.0	23.8	35.0
100-101	29.738968625	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33.0
3	9.0
4	34.0
5	100.0
6	294.0
7	860.0
8	2042.0
9	3331.0
10	4306.0
11	4966.0
12	5072.0
13	5359.0
14	5655.0
15	6079.0
16	6622.0
17	7171.0
18	8100.0
19	8773.0
20	9419.0
21	10690.0
22	12243.0
23	15136.0
24	18767.0
25	23263.0
26	29424.0
27	36549.0
28	45638.0
29	57173.0
30	71859.0
31	92475.0
32	121891.0
33	171211.0
34	280248.0
35	684249.0
36	1415479.0
37	706028.0
38	116792.0
39	12639.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.290086260782594	23.104688086010754	13.8512064008001	25.75401925240655
2	31.0065	24.049300000000002	19.830075	25.114124999999998
3	28.28755	19.798425	21.637600000000003	30.276425
4	30.679511257517845	19.76518419847313	19.988269460395177	29.567035083613845
5	31.254324999999998	20.6206	20.056775	28.0683
6	29.662175	19.11775	17.812375	33.4077
7	32.758925	18.864675000000002	19.154075000000002	29.222324999999998
8	28.58395	19.558600000000002	19.937225	31.920225000000002
9	30.6929	17.874850000000002	19.269949999999998	32.1623
10-14	30.827935	19.238084999999998	19.211714999999998	30.722265
15-19	31.021205000000002	18.803415	18.979925	31.195455
20-24	30.925629347254883	18.867187441405342	19.018839792016777	31.188343419323
25-29	31.080896080896082	18.983148983148983	18.954283954283955	30.981670981670984
30-34	31.251381529278067	18.760245951353262	18.747560517547694	31.240812001820977
35-39	31.089261119847304	18.83719381183284	18.891976311557052	31.1815687567628
40-44	31.222231588985366	18.891209579458668	18.81995884557611	31.066599985979853
45-49	31.33822	18.689664999999998	18.675805	31.29631
50-54	31.129630000000002	18.764085	18.83616	31.270125
55-59	31.26483595456135	18.846731673741424	18.78166159891084	31.10677077278639
60-64	31.36848783643175	18.642646482570587	18.640445628859435	31.348420052138227
65-69	31.159555979004278	18.74043676901956	18.81128607545556	31.2887211765206
70-74	31.32797	18.81634	18.73663	31.119059999999998
75-79	31.394355	18.626545	18.628220000000002	31.350879999999997
80-84	31.215154882799002	18.73560406836666	18.79044523648354	31.258795812350805
85-89	31.30796574761735	18.79649713729882	18.74601124433972	31.14952587074411
90-94	31.431223214178527	18.60989339693236	18.58549854141073	31.37338484747838
95-99	31.280805271433398	18.68448871862171	18.727059957444762	31.307646052500125
100-101	31.565642045389204	18.817472178624804	18.67508403787817	30.94180173810782
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.04410551318915
2	56.120625000000004
3	58.563975
4	60.24654634113169
5	59.322624999999995
6	63.069874999999996
7	61.981249999999996
8	60.504175000000004
9	62.855199999999996
10-14	61.5502
15-19	62.216660000000005
20-24	62.11397276657789
25-29	62.06256706256706
30-34	62.49219353109905
35-39	62.270829876610115
40-44	62.28883157496522
45-49	62.63453
50-54	62.399755
55-59	62.37160672734774
60-64	62.71690788856997
65-69	62.44827715552488
70-74	62.447030000000005
75-79	62.745235
80-84	62.4739506951498
85-89	62.45749161836146
90-94	62.80460806165691
95-99	62.588451323933526
100-101	62.50744378349703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	4.0
2	0.5
3	1.5
4	3.0
5	1.5
6	2.5
7	4.5
8	5.0
9	6.5
10	12.5
11	21.5
12	23.5
13	31.5
14	42.5
15	57.5
16	75.5
17	103.5
18	150.5
19	205.5
20	285.5
21	380.5
22	479.5
23	623.0
24	808.5
25	1090.0
26	1420.0
27	1798.0
28	2296.5
29	2803.0
30	3255.5
31	3837.0
32	4551.0
33	5362.5
34	6218.5
35	6885.0
36	7688.5
37	8569.5
38	9238.5
39	10019.5
40	11022.5
41	12137.5
42	13324.0
43	14948.5
44	17079.5
45	19874.0
46	23649.5
47	27976.5
48	33499.0
49	40311.5
50	48026.5
51	56942.5
52	66989.5
53	77817.5
54	89246.0
55	100916.5
56	112396.0
57	124044.5
58	135513.5
59	146913.0
60	157683.0
61	168118.0
62	178562.0
63	187536.5
64	194542.0
65	198797.0
66	199928.0
67	197445.5
68	190742.5
69	180815.5
70	167014.0
71	149477.5
72	130093.5
73	110161.5
74	90574.0
75	71864.5
76	54939.0
77	40325.0
78	28432.5
79	19434.0
80	12787.5
81	7878.0
82	4644.5
83	2727.5
84	1584.5
85	919.0
86	598.0
87	415.5
88	295.0
89	214.5
90	150.0
91	103.5
92	75.5
93	54.0
94	34.5
95	17.5
96	9.5
97	7.0
98	3.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0125
2	0.0
3	0.0
4	0.0046
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0015500000000000002
25-29	9.999999999999999E-5
30-34	0.042835
35-39	0.013689999999999999
40-44	0.0010299999999999999
45-49	0.0
50-54	0.0
55-59	1.15E-4
60-64	0.03879
65-69	0.02725
70-74	0.0
75-79	0.0
80-84	0.00213
85-89	0.03148
90-94	0.0404
95-99	0.00291
100-101	4.5E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.14998050253466935
#Duplication Level	Relative count
1	100.0
2	0.13368314590989655
3	0.0025034296986872016
4	5.006859397374403E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.006859397374403E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	295390	3.206178	3.5841236	10-14
AAAAA	294915	3.129756	3.6490462	1
>>END_MODULE