##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81124175	34.0	31.0	34.0	31.0	34.0
2	33.03450375	34.0	31.0	34.0	31.0	34.0
3	33.07909475	34.0	33.0	34.0	31.0	34.0
4	36.46634675	37.0	37.0	37.0	35.0	37.0
5	36.398131	37.0	37.0	37.0	35.0	37.0
6	36.37232625	37.0	37.0	37.0	35.0	37.0
7	36.36682175	37.0	37.0	37.0	35.0	37.0
8	36.34904375	37.0	37.0	37.0	35.0	37.0
9	38.14803625	39.0	39.0	39.0	37.0	39.0
10-14	38.4084501	39.4	38.6	39.4	37.0	39.4
15-19	39.55405954999999	41.0	40.0	41.0	37.0	41.0
20-24	39.324653	40.8	39.0	41.0	36.2	41.0
25-29	38.900954350000006	40.0	38.4	41.0	35.0	41.0
30-34	38.17539839999999	40.0	37.2	41.0	34.0	41.0
35-39	37.1737613	38.6	35.2	40.6	32.8	41.0
40-44	36.187082	36.6	35.0	40.0	32.0	41.0
45-49	35.59842885	35.0	35.0	38.8	32.4	41.0
50-54	34.7433039	35.0	35.0	36.6	31.4	40.0
55-59	34.0897427	35.0	34.2	35.0	31.0	38.0
60-64	33.587064999999996	35.0	34.0	35.0	30.4	36.2
65-69	33.2168251	35.0	33.2	35.0	29.6	35.0
70-74	32.962443	35.0	33.0	35.0	29.0	35.0
75-79	32.32717685	34.4	32.6	35.0	27.8	35.0
80-84	32.6139641	35.0	33.0	35.0	29.0	35.0
85-89	32.3359958	35.0	33.0	35.0	27.6	35.0
90-94	31.91140685	35.0	33.0	35.0	27.0	35.0
95-99	31.423066950000003	35.0	33.0	35.0	24.6	35.0
100-101	30.14007625	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28.0
3	6.0
4	22.0
5	82.0
6	281.0
7	809.0
8	1716.0
9	2788.0
10	3489.0
11	3932.0
12	4103.0
13	4280.0
14	4668.0
15	4966.0
16	5690.0
17	6289.0
18	6958.0
19	7419.0
20	8223.0
21	9242.0
22	11200.0
23	13734.0
24	17102.0
25	22143.0
26	28174.0
27	35237.0
28	43711.0
29	55172.0
30	69070.0
31	89046.0
32	118993.0
33	165891.0
34	273066.0
35	683667.0
36	1454733.0
37	716458.0
38	115446.0
39	12155.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.243663170286446	23.207408135316392	13.823466679869046	25.725462014528116
2	30.96705	24.049300000000002	19.84785	25.135800000000003
3	28.309050000000003	19.829325	21.58475	30.276874999999997
4	30.69165	19.79005	19.996625	29.521675000000002
5	31.254875	20.6093	20.003675	28.13215
6	29.706500000000002	19.067825	17.813200000000002	33.412475
7	32.75175	18.8561	19.19315	29.199
8	28.540525	19.510875	19.968125	31.980475000000002
9	30.704725	17.8122	19.288574999999998	32.1945
10-14	30.83684	19.227775	19.194005	30.741380000000003
15-19	31.056385	18.791345	18.974185	31.178085
20-24	30.92592651852717	18.86647045046858	19.01608263480647	31.191520396197784
25-29	31.10274899623705	18.985918543663345	18.927938517572333	30.983393942527275
30-34	31.259426217742043	18.742929555187057	18.72545882303984	31.27218540403106
35-39	31.076688018757757	18.817401569220188	18.881309834932566	31.22460057708949
40-44	31.228596228596228	18.872438872438874	18.803183803183803	31.095781095781096
45-49	31.359865	18.64662	18.661625	31.33189
50-54	31.146479999999997	18.743655	18.821035	31.28883
55-59	31.254090938931828	18.819301586755554	18.792256577289802	31.134350897022816
60-64	31.35539519856909	18.63744066700893	18.638351192473177	31.368812941948796
65-69	31.167705602691605	18.730264850656113	18.794799864427453	31.30722968222483
70-74	31.289	18.811349999999997	18.763659999999998	31.135990000000003
75-79	31.39529	18.613605	18.626435	31.36467
80-84	31.192891946574992	18.71888043938192	18.785806215076363	31.30242139896673
85-89	31.30504935853491	18.79745187979972	18.733450203639197	31.164048558026174
90-94	31.426083791323826	18.602766883247668	18.605768514033674	31.365380811394832
95-99	31.249430801603882	18.68776670430065	18.751922021382605	31.31088047271286
100-101	31.520621449787832	18.8366841777758	18.68874434772348	30.95395002471289
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.969125184814565
2	56.102850000000004
3	58.585925
4	60.213325
5	59.387025
6	63.118975
7	61.95075
8	60.521
9	62.899225
10-14	61.578219999999995
15-19	62.234469999999995
20-24	62.11744691472495
25-29	62.08614293876432
30-34	62.53161162177311
35-39	62.30128859584725
40-44	62.32437732437732
45-49	62.69175500000001
50-54	62.43531
55-59	62.388441835954644
60-64	62.72420814051789
65-69	62.474935284916434
70-74	62.42499
75-79	62.75996000000001
80-84	62.495313345541724
85-89	62.46909791656109
90-94	62.79146460271866
95-99	62.56031127431674
100-101	62.47457147450073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.5
4	1.5
5	1.5
6	3.0
7	3.0
8	6.5
9	8.5
10	10.0
11	15.5
12	26.0
13	33.0
14	40.5
15	57.0
16	79.0
17	103.5
18	142.0
19	202.5
20	267.0
21	342.0
22	453.5
23	633.0
24	829.5
25	1055.5
26	1384.5
27	1778.5
28	2200.0
29	2707.5
30	3283.5
31	3875.0
32	4543.0
33	5298.5
34	6134.0
35	6866.5
36	7566.0
37	8344.0
38	9086.0
39	9917.0
40	10932.0
41	12081.5
42	13411.5
43	15052.0
44	17159.5
45	19882.0
46	23329.0
47	27865.0
48	33410.5
49	39977.0
50	47644.5
51	56600.0
52	66777.0
53	77514.0
54	89023.5
55	101031.0
56	112251.0
57	123965.0
58	135668.0
59	146533.0
60	157810.0
61	168677.5
62	178892.5
63	187412.5
64	194527.5
65	199075.0
66	199792.5
67	197438.5
68	191294.5
69	181194.0
70	167094.5
71	149880.5
72	130851.0
73	111346.0
74	91421.0
75	72376.5
76	55619.5
77	40595.0
78	28396.5
79	19161.0
80	12267.0
81	7495.0
82	4342.5
83	2406.5
84	1298.5
85	718.0
86	404.5
87	257.0
88	172.5
89	113.0
90	77.5
91	62.0
92	47.0
93	31.0
94	17.5
95	10.0
96	6.0
97	3.0
98	2.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0014600000000000001
25-29	4.4999999999999996E-5
30-34	0.03281
35-39	0.00511
40-44	9.999999999999999E-5
45-49	0.0
50-54	0.0
55-59	3.5E-5
60-64	0.05771
65-69	0.038759999999999996
70-74	0.0
75-79	0.0
80-84	0.00863
85-89	0.057304999999999995
90-94	0.05433
95-99	0.023875
100-101	0.001575
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1504849515048554
#Duplication Level	Relative count
1	100.0
2	0.1371927558219298
3	0.003504924418808426
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	280765	3.0552804	3.5456517	10-14
>>END_MODULE