##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.821209	34.0	31.0	34.0	31.0	34.0
2	33.0493535	34.0	31.0	34.0	31.0	34.0
3	33.0893435	34.0	33.0	34.0	31.0	34.0
4	36.481406	37.0	37.0	37.0	35.0	37.0
5	36.41888225	37.0	37.0	37.0	35.0	37.0
6	36.38957425	37.0	37.0	37.0	35.0	37.0
7	36.37852525	37.0	37.0	37.0	35.0	37.0
8	36.35841975	37.0	37.0	37.0	35.0	37.0
9	38.15321	39.0	39.0	39.0	37.0	39.0
10-14	38.417592150000004	39.4	38.6	39.4	37.0	39.4
15-19	39.55899505	41.0	40.0	41.0	37.0	41.0
20-24	39.3101468	40.8	39.0	41.0	36.2	41.0
25-29	38.85975115	40.0	38.2	41.0	35.0	41.0
30-34	38.1035128	40.0	37.0	41.0	33.8	41.0
35-39	37.044047850000005	38.6	35.2	40.4	32.6	41.0
40-44	36.10186365	36.6	35.0	40.0	31.8	41.0
45-49	35.509797500000005	35.0	35.0	38.8	32.0	41.0
50-54	34.613239799999995	35.0	34.6	36.6	31.2	39.8
55-59	33.9407553	35.0	34.0	35.0	30.8	38.0
60-64	33.4258271	35.0	33.8	35.0	29.8	36.2
65-69	33.02626475000001	35.0	33.0	35.0	29.0	35.0
70-74	32.717468350000004	35.0	33.0	35.0	29.0	35.0
75-79	32.082559249999996	34.4	32.4	35.0	26.8	35.0
80-84	32.29122815	35.0	33.0	35.0	27.0	35.0
85-89	31.98175145	35.0	33.0	35.0	27.0	35.0
90-94	31.544689499999997	35.0	33.0	35.0	25.0	35.0
95-99	30.94368775	35.0	32.2	35.0	23.0	35.0
100-101	29.4974335	34.0	29.5	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26.0
3	13.0
4	30.0
5	95.0
6	259.0
7	758.0
8	1903.0
9	3102.0
10	4047.0
11	4638.0
12	4757.0
13	5068.0
14	5494.0
15	5878.0
16	6411.0
17	7282.0
18	8051.0
19	8738.0
20	9886.0
21	10763.0
22	12462.0
23	15387.0
24	19040.0
25	23757.0
26	29763.0
27	37614.0
28	46630.0
29	57234.0
30	73642.0
31	94402.0
32	125721.0
33	176852.0
34	293332.0
35	715213.0
36	1407963.0
37	665014.0
38	107845.0
39	10918.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.30615575948237	23.162970930677385	13.842420278266593	25.688453031573648
2	30.96515	24.070275	19.83175	25.132824999999997
3	28.241625	19.792375	21.625525	30.340475
4	30.665225000000003	19.795650000000002	19.975175	29.56395
5	31.193975000000002	20.659325	20.028950000000002	28.11775
6	29.655775000000002	19.0942	17.836199999999998	33.413825
7	32.785599999999995	18.858575	19.14575	29.210075000000003
8	28.563149999999997	19.5459	19.916675	31.974275000000002
9	30.643775	17.90515	19.2483	32.202775
10-14	30.831955	19.22814	19.19228	30.747625
15-19	31.036540000000002	18.810805	18.962680000000002	31.189974999999997
20-24	30.952713336590698	18.858069809084128	18.98833108564464	31.200885768680532
25-29	31.110445882669	18.99169257232555	18.931207006785513	30.966654538219935
30-34	31.258326237115625	18.73257399551625	18.753319864276893	31.25577990309123
35-39	31.08467012057613	18.802502592532328	18.875285518592975	31.237541768298566
40-44	31.235302223681806	18.8597795638558	18.799983439688667	31.104934772773728
45-49	31.353334999999998	18.650325000000002	18.663285	31.333054999999998
50-54	31.14415773444488	18.748470601294322	18.816101649575568	31.291270014685228
55-59	31.273255729650785	18.83548975970392	18.749864374389684	31.141390136255616
60-64	31.368833466385627	18.628079933829703	18.642012154899884	31.361074444884785
65-69	31.17645755312784	18.7250519114327	18.791025738696522	31.307464796742934
70-74	31.3271	18.779885	18.72723	31.165785
75-79	31.42598	18.585189999999997	18.60657	31.382260000000002
80-84	31.225997511144715	18.714101437980272	18.754537210186754	31.305363840688262
85-89	31.33576538746462	18.78353372592964	18.717747507079487	31.162953379526254
90-94	31.43948494186604	18.58615069330785	18.58293873433407	31.391425630492037
95-99	31.299192692978785	18.65481336119875	18.72239150514775	31.323602440674712
100-101	31.59660672242962	18.791152678115512	18.608126191025303	31.00411440842957
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.99460879105603
2	56.097975
3	58.582100000000004
4	60.229175
5	59.311725
6	63.0696
7	61.99567499999999
8	60.537425
9	62.84655
10-14	61.57958
15-19	62.226515000000006
20-24	62.153599105271226
25-29	62.07710042088893
30-34	62.51410614020686
35-39	62.322211888874705
40-44	62.34023699645553
45-49	62.68639
50-54	62.43542774913011
55-59	62.414645865906394
60-64	62.72990791127041
65-69	62.48392234987078
70-74	62.492885
75-79	62.808240000000005
80-84	62.53136135183297
85-89	62.498718766990876
90-94	62.83091057235808
95-99	62.6227951336535
100-101	62.60072113085919
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	4.5
2	2.0
3	1.0
4	2.0
5	3.0
6	3.0
7	4.0
8	7.0
9	10.0
10	10.0
11	15.0
12	20.0
13	19.5
14	38.5
15	57.0
16	79.5
17	118.0
18	146.0
19	199.0
20	274.0
21	356.5
22	472.5
23	636.0
24	834.5
25	1061.0
26	1366.0
27	1763.0
28	2250.5
29	2752.5
30	3281.5
31	3957.5
32	4604.0
33	5250.0
34	6101.0
35	6897.5
36	7567.0
37	8246.0
38	9037.5
39	10017.5
40	10886.0
41	11895.5
42	13136.5
43	14840.5
44	17004.5
45	19736.5
46	23379.0
47	27796.0
48	33588.0
49	40345.5
50	47955.0
51	56767.5
52	66564.0
53	77380.5
54	88985.5
55	100741.0
56	112218.5
57	123722.5
58	135182.5
59	146396.0
60	157690.0
61	168524.0
62	178550.5
63	187333.0
64	193951.0
65	198796.0
66	200349.5
67	197626.0
68	191501.5
69	181462.5
70	167096.0
71	149518.0
72	130569.5
73	110783.0
74	90773.0
75	72166.0
76	55413.0
77	40894.5
78	29031.5
79	19627.0
80	12752.5
81	7872.5
82	4649.5
83	2768.5
84	1599.0
85	905.5
86	576.5
87	396.0
88	272.0
89	187.5
90	131.0
91	89.5
92	58.5
93	43.0
94	26.5
95	13.5
96	8.5
97	4.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01315
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	9.8E-4
25-29	9.350000000000001E-4
30-34	0.05239
35-39	0.01089
40-44	0.00188
45-49	0.0
50-54	0.0015500000000000002
55-59	4.5E-4
60-64	0.051829999999999994
65-69	0.043695000000000005
70-74	0.0
75-79	0.0
80-84	0.014275
85-89	0.047455000000000004
90-94	0.06099
95-99	0.019450000000000002
100-101	8.125E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.15748582627562574
#Duplication Level	Relative count
1	100.0
2	0.14571638883742358
3	0.0020029744170092585
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010014872085046293
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	282495	3.0890868	3.562854	10-14
>>END_MODULE