##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002007_ItsyBitsy_ATTCCT_L003_R1_003.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 62 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81257475 34.0 31.0 34.0 31.0 34.0 2 33.03958175 34.0 31.0 34.0 31.0 34.0 3 33.09060025 34.0 33.0 34.0 31.0 34.0 4 36.46406725 37.0 37.0 37.0 35.0 37.0 5 36.40572925 37.0 37.0 37.0 35.0 37.0 6 36.3762895 37.0 37.0 37.0 35.0 37.0 7 36.375333 37.0 37.0 37.0 35.0 37.0 8 36.347891 37.0 37.0 37.0 35.0 37.0 9 38.1444625 39.0 39.0 39.0 37.0 39.0 10-14 38.409168199999996 39.4 38.6 39.4 37.0 39.4 15-19 39.541634 41.0 39.8 41.0 37.0 41.0 20-24 39.28772635 40.6 39.0 41.0 36.0 41.0 25-29 38.822190899999995 40.0 38.2 41.0 35.0 41.0 30-34 38.05113815 40.0 37.0 41.0 33.6 41.0 35-39 37.027592500000004 38.6 35.2 40.4 32.6 41.0 40-44 36.068021449999996 36.6 35.0 40.0 31.6 41.0 45-49 35.495567300000005 35.0 35.0 38.8 32.0 41.0 50-54 34.610646200000005 35.0 34.8 36.6 31.0 40.0 55-59 33.922690550000006 35.0 34.0 35.0 30.6 38.0 60-64 33.37266425 35.0 33.4 35.0 29.8 36.2 65-69 32.94199245 35.0 33.0 35.0 29.0 35.0 70-74 32.6591607 35.0 33.0 35.0 28.6 35.0 75-79 32.036325149999996 34.4 32.4 35.0 26.4 35.0 80-84 32.21353455 35.0 33.0 35.0 27.0 35.0 85-89 31.904996550000003 35.0 33.0 35.0 26.6 35.0 90-94 31.42679285 35.0 33.0 35.0 24.2 35.0 95-99 30.808310849999998 35.0 32.0 35.0 22.2 35.0 100-101 29.4689295 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27.0 3 9.0 4 31.0 5 99.0 6 313.0 7 914.0 8 2095.0 9 3220.0 10 4222.0 11 4973.0 12 5066.0 13 5071.0 14 5703.0 15 6319.0 16 6859.0 17 7547.0 18 8486.0 19 9291.0 20 10165.0 21 11301.0 22 13095.0 23 15668.0 24 19548.0 25 24977.0 26 31142.0 27 38555.0 28 47275.0 29 59313.0 30 74430.0 31 95926.0 32 127460.0 33 179850.0 34 297486.0 35 703450.0 36 1385672.0 37 672934.0 38 110177.0 39 11321.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.24970537396881 23.167181085133798 13.811048338669185 25.772065202228205 2 31.022575000000003 24.0746 19.827749999999998 25.075075000000002 3 28.3121 19.8397 21.58025 30.26795 4 30.679475 19.817675 19.956075000000002 29.546774999999997 5 31.1996 20.660975 20.04595 28.093475 6 29.710449999999998 19.105825000000003 17.767875 33.415850000000006 7 32.754475 18.863675 19.182775 29.199075 8 28.594625 19.525925 19.943025 31.936425 9 30.682850000000002 17.902675000000002 19.287475 32.127 10-14 30.82936 19.226685 19.215545 30.72841 15-19 31.050565000000002 18.81004 18.96932 31.170075000000004 20-24 30.93012523137755 18.87075257827836 19.005234057574633 31.19388813276946 25-29 31.112366566917434 18.997616928433203 18.919326219902292 30.970690284747075 30-34 31.261487355265704 18.749475260463445 18.741724876271544 31.247312507999307 35-39 31.086377288780113 18.817505698508636 18.88502764989403 31.21108936281722 40-44 31.226441226441224 18.871658871658873 18.81806381806382 31.083836083836086 45-49 31.34613 18.663495 18.665895 31.324479999999998 50-54 31.142821771188046 18.746877478766855 18.82763302790459 31.282667722140513 55-59 31.260113756068254 18.845166307099785 18.768146260887754 31.126573675944208 60-64 31.381031620636012 18.62824858463112 18.618002837791717 31.372716956941147 65-69 31.18181227806517 18.721856484166985 18.803453355297965 31.292877882469888 70-74 31.310755 18.816345000000002 18.717675 31.155224999999998 75-79 31.40847 18.61629 18.60569 31.36955 80-84 31.222481239968314 18.708183125296422 18.76716355162693 31.30217208310834 85-89 31.33160234197108 18.799352614166487 18.718329331528903 31.150715712333525 90-94 31.469336344140412 18.56907526729112 18.58510450205305 31.376483886515423 95-99 31.294303909358735 18.680458337402932 18.719906548548078 31.305331204690255 100-101 31.596870526920135 18.820326299906462 18.62542486250836 30.95737831066504 >>END_MODULE >>Per base GC content warn #Base %GC 1 63.021770576197014 2 56.09765 3 58.58004999999999 4 60.22625 5 59.293074999999995 6 63.1263 7 61.95355 8 60.53105 9 62.80985 10-14 61.55777 15-19 62.22064 20-24 62.124013364147004 25-29 62.08305685166451 30-34 62.50879986326501 35-39 62.29746665159733 40-44 62.31027731027731 45-49 62.67061 50-54 62.425489493328556 55-59 62.38668743201246 60-64 62.75374857757716 65-69 62.474690160535054 70-74 62.46598 75-79 62.77802 80-84 62.52465332307665 85-89 62.482318054304606 90-94 62.84582023065583 95-99 62.59963511404899 100-101 62.55424883758518 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4.0 1 3.5 2 4.0 3 3.0 4 1.5 5 2.0 6 2.5 7 3.0 8 6.0 9 8.0 10 10.5 11 16.0 12 22.5 13 31.0 14 44.0 15 66.0 16 79.0 17 114.0 18 158.0 19 211.0 20 283.0 21 356.0 22 489.0 23 636.5 24 812.0 25 1088.5 26 1380.0 27 1713.0 28 2177.0 29 2694.5 30 3269.0 31 3850.0 32 4528.5 33 5356.0 34 6133.0 35 6841.0 36 7571.0 37 8342.0 38 9182.0 39 10035.5 40 10923.0 41 11949.5 42 13274.0 43 14905.0 44 17006.0 45 19792.0 46 23394.5 47 27957.0 48 33477.0 49 40128.5 50 47948.5 51 56875.5 52 67049.5 53 77961.0 54 89197.0 55 100673.5 56 112094.5 57 123919.5 58 135560.0 59 147140.5 60 158145.5 61 168893.0 62 179020.0 63 187295.0 64 194620.0 65 199296.5 66 199622.0 67 196845.5 68 190647.5 69 180312.0 70 166431.5 71 149678.0 72 130911.5 73 110677.5 74 90500.0 75 72043.0 76 55038.5 77 40226.5 78 28699.5 79 19630.5 80 12855.5 81 8002.0 82 4794.0 83 2777.5 84 1559.0 85 935.0 86 597.0 87 397.5 88 264.0 89 174.5 90 124.0 91 80.5 92 55.0 93 40.5 94 25.5 95 16.5 96 9.0 97 5.5 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.5E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0011 25-29 9.05E-4 30-34 0.06947 35-39 0.0177 40-44 9.999999999999999E-5 45-49 0.0 50-54 6.8E-4 55-59 5.9999999999999995E-5 60-64 0.05609 65-69 0.06357 70-74 0.0 75-79 0.0 80-84 0.026154999999999998 85-89 0.053419999999999995 90-94 0.08880500000000001 95-99 0.020815 100-101 7.375E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.16148788840837078 #Duplication Level Relative count 1 100.0 2 0.15223444103920036 3 0.0015023135628868458 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10++ 5.007711876289486E-4 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 281835 3.0819075 3.5360577 10-14 >>END_MODULE