##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8053905	34.0	31.0	34.0	31.0	34.0
2	33.032001	34.0	31.0	34.0	31.0	34.0
3	33.073443	34.0	31.0	34.0	31.0	34.0
4	36.46241175	37.0	37.0	37.0	35.0	37.0
5	36.38853525	37.0	37.0	37.0	35.0	37.0
6	36.35953125	37.0	37.0	37.0	35.0	37.0
7	36.34579575	37.0	37.0	37.0	35.0	37.0
8	36.32225	37.0	37.0	37.0	35.0	37.0
9	38.11360025	39.0	39.0	39.0	37.0	39.0
10-14	38.397676849999996	39.4	38.6	39.4	37.0	39.4
15-19	39.533820500000004	41.0	39.8	41.0	37.0	41.0
20-24	39.284142100000004	40.8	39.0	41.0	36.0	41.0
25-29	38.8493889	40.0	38.2	41.0	35.0	41.0
30-34	38.1118482	40.0	37.0	41.0	33.6	41.0
35-39	37.092291349999996	38.6	35.2	40.6	32.6	41.0
40-44	36.09073595	36.6	35.0	40.0	31.8	41.0
45-49	35.5052784	35.0	35.0	38.8	32.0	41.0
50-54	34.6179941	35.0	34.8	36.6	31.0	40.0
55-59	33.9337864	35.0	34.0	35.0	30.6	38.0
60-64	33.41587705	35.0	33.8	35.0	29.8	36.2
65-69	33.03947125	35.0	33.0	35.0	29.0	35.0
70-74	32.739447	35.0	33.0	35.0	29.0	35.0
75-79	32.07783005	34.4	32.4	35.0	26.6	35.0
80-84	32.3515534	35.0	33.0	35.0	27.0	35.0
85-89	32.04715695	35.0	33.0	35.0	27.0	35.0
90-94	31.58258915	35.0	33.0	35.0	25.0	35.0
95-99	30.9934613	35.0	32.4	35.0	23.4	35.0
100-101	29.674166875	34.0	30.5	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26.0
3	13.0
4	34.0
5	100.0
6	279.0
7	827.0
8	1924.0
9	3154.0
10	4135.0
11	4671.0
12	4969.0
13	5118.0
14	5378.0
15	6002.0
16	6639.0
17	7288.0
18	7952.0
19	8802.0
20	9540.0
21	10641.0
22	12602.0
23	15534.0
24	18947.0
25	24057.0
26	30335.0
27	37705.0
28	47360.0
29	58781.0
30	74306.0
31	95877.0
32	126702.0
33	175899.0
34	285522.0
35	680530.0
36	1407421.0
37	695115.0
38	113787.0
39	12009.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.229352576705004	23.170690233762155	13.857788229222045	25.742168960310796
2	31.004775000000002	24.026149999999998	19.818649999999998	25.150425
3	28.286450000000002	19.810725	21.591949999999997	30.310875
4	30.612650000000002	19.83005	19.955524999999998	29.601775000000004
5	31.224800000000002	20.6853	20.017725	28.072174999999998
6	29.69975	19.0874	17.784875	33.427974999999996
7	32.754575	18.885425	19.220724999999998	29.139274999999998
8	28.576475000000002	19.539225	19.964975	31.919324999999997
9	30.644525	17.897875	19.272025	32.185575
10-14	30.822480000000002	19.2422	19.205145	30.730175
15-19	31.029230000000002	18.805934999999998	18.989535	31.1753
20-24	30.938684774807008	18.872561262349784	19.001272259860013	31.1874817029832
25-29	31.079162280795614	18.99063831948955	18.945377930250203	30.984821469464634
30-34	31.250274209819224	18.7668174726872	18.75597164576669	31.226936671726886
35-39	31.089123200344698	18.82678811842687	18.882662209141426	31.201426472087007
40-44	31.225427891639036	18.903570755146482	18.800640850536528	31.07036050267795
45-49	31.337605000000003	18.662235	18.66407	31.33609
50-54	31.161950615893808	18.74337303023433	18.810938996427648	31.283737357444213
55-59	31.264789999999998	18.84628	18.783659999999998	31.10527
60-64	31.385963140800342	18.629298044012728	18.631318531859517	31.35342028332741
65-69	31.173367141670877	18.73763990189394	18.799897710740158	31.28909524569503
70-74	31.316475	18.813755	18.740245	31.129525
75-79	31.39442	18.635975	18.60695	31.362655
80-84	31.218182452790543	18.730367417631538	18.749649340039202	31.30180078953872
85-89	31.345377938943315	18.805221307325283	18.710319726705855	31.139081027025544
90-94	31.44821954389388	18.597585542969224	18.577702909031924	31.37649200410498
95-99	31.300725741617264	18.659126966803488	18.722174491525564	31.317972800053685
100-101	31.590353349355084	18.831548865631547	18.662385477523756	30.915712307489613
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.971521537015796
2	56.15520000000001
3	58.597325
4	60.214425000000006
5	59.296974999999996
6	63.127725
7	61.89384999999999
8	60.4958
9	62.8301
10-14	61.552655
15-19	62.204530000000005
20-24	62.1261664777902
25-29	62.063983750260256
30-34	62.47721088154611
35-39	62.29054967243171
40-44	62.295788394316986
45-49	62.673694999999995
50-54	62.44568797333802
55-59	62.37006
60-64	62.73938342412776
65-69	62.4624623873659
70-74	62.446
75-79	62.75707499999999
80-84	62.51998324232926
85-89	62.484458965968855
90-94	62.82471154799886
95-99	62.618698541670945
100-101	62.5060656568447
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	2.5
4	3.0
5	2.0
6	2.5
7	4.5
8	7.5
9	8.5
10	8.5
11	13.5
12	23.0
13	32.0
14	41.5
15	57.5
16	75.0
17	103.0
18	150.0
19	204.0
20	272.0
21	374.5
22	495.0
23	640.5
24	820.5
25	1034.0
26	1372.5
27	1801.0
28	2214.5
29	2757.0
30	3304.0
31	3900.5
32	4643.0
33	5351.5
34	6016.5
35	6796.5
36	7573.5
37	8364.5
38	9228.0
39	10090.5
40	10901.5
41	11897.5
42	13320.0
43	14925.5
44	17052.0
45	19758.0
46	23196.5
47	27841.5
48	33307.5
49	40042.0
50	48269.0
51	57187.5
52	66921.5
53	77920.0
54	89152.5
55	100576.5
56	112172.5
57	123741.0
58	135491.5
59	146831.5
60	157920.0
61	168772.5
62	178874.5
63	187523.5
64	194269.5
65	198703.0
66	200328.5
67	197749.5
68	191227.5
69	181034.5
70	167182.5
71	150307.0
72	130599.0
73	110066.0
74	90313.5
75	71626.0
76	54710.0
77	40368.5
78	28700.5
79	19485.0
80	12560.0
81	7731.5
82	4628.0
83	2676.5
84	1546.0
85	959.0
86	566.5
87	367.5
88	274.5
89	188.0
90	149.0
91	105.0
92	65.5
93	52.5
94	34.5
95	15.5
96	10.5
97	7.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02445
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	7.750000000000001E-4
25-29	8.600000000000001E-4
30-34	0.053724999999999995
35-39	0.01627
40-44	0.004765
45-49	0.0
50-54	0.00143
55-59	0.0
60-64	0.024145
65-69	0.028605
70-74	0.0
75-79	0.0
80-84	0.00997
85-89	0.02274
90-94	0.038395
95-99	0.011935
100-101	5.25E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.13498380194377388
#Duplication Level	Relative count
1	100.0
2	0.11964716601419746
3	0.0025030787869078966
4	0.0
5	5.006157573815794E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.006157573815794E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	281250	3.0665085	3.6204226	10-14
>>END_MODULE