##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.829077	34.0	31.0	34.0	31.0	34.0
2	33.05067925	34.0	31.0	34.0	31.0	34.0
3	33.1053575	34.0	33.0	34.0	31.0	34.0
4	36.46360075	37.0	37.0	37.0	35.0	37.0
5	36.40688275	37.0	37.0	37.0	35.0	37.0
6	36.37348125	37.0	37.0	37.0	35.0	37.0
7	36.36802775	37.0	37.0	37.0	35.0	37.0
8	36.34279375	37.0	37.0	37.0	35.0	37.0
9	38.152365	39.0	39.0	39.0	37.0	39.0
10-14	38.419113599999996	39.4	39.0	39.4	37.0	39.4
15-19	39.56100665	41.0	40.0	41.0	37.0	41.0
20-24	39.352768	41.0	39.0	41.0	36.2	41.0
25-29	38.908627949999996	40.0	38.4	41.0	35.0	41.0
30-34	38.150282000000004	40.0	37.2	41.0	33.8	41.0
35-39	37.14900630000001	38.6	35.2	40.6	32.8	41.0
40-44	36.1627699	36.8	35.0	40.0	31.8	41.0
45-49	35.537968	35.0	35.0	39.0	32.0	41.0
50-54	34.6412648	35.0	35.0	36.6	31.0	40.0
55-59	33.97055114999999	35.0	34.0	35.0	30.8	38.0
60-64	33.4306241	35.0	34.0	35.0	30.0	36.4
65-69	33.03332155	35.0	33.0	35.0	29.0	35.0
70-74	32.7393414	35.0	33.0	35.0	29.0	35.0
75-79	32.102157950000006	34.4	32.6	35.0	27.0	35.0
80-84	32.34405115	35.0	33.0	35.0	27.0	35.0
85-89	32.0136184	35.0	33.0	35.0	27.0	35.0
90-94	31.6042792	35.0	33.0	35.0	25.0	35.0
95-99	30.962442099999997	35.0	32.6	35.0	22.8	35.0
100-101	29.761134875	34.0	30.5	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32.0
3	12.0
4	36.0
5	97.0
6	281.0
7	840.0
8	1982.0
9	3138.0
10	4144.0
11	4702.0
12	4959.0
13	5201.0
14	5625.0
15	6041.0
16	6860.0
17	7742.0
18	8222.0
19	9018.0
20	9945.0
21	11021.0
22	12849.0
23	15594.0
24	19227.0
25	24064.0
26	30046.0
27	37292.0
28	46119.0
29	58404.0
30	73051.0
31	93575.0
32	123431.0
33	173166.0
34	280338.0
35	662223.0
36	1393351.0
37	729615.0
38	123461.0
39	14268.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.264238816479924	23.08292247221327	13.809509639743334	25.843329071563474
2	31.059124999999998	24.008	19.8129	25.119975
3	28.317725	19.8015	21.596575	30.2842
4	30.680075000000002	19.79975	19.942525	29.57765
5	31.228675	20.630775	20.079325	28.061225
6	29.686525000000003	19.1002	17.814375	33.3989
7	32.760625	18.8541	19.198775	29.1865
8	28.59115	19.5094	19.9838	31.91565
9	30.68615	17.9005	19.298825	32.114525
10-14	30.817675	19.25259	19.228505000000002	30.701230000000002
15-19	31.03727	18.823895	18.98258	31.156254999999998
20-24	30.92959472142789	18.895894733363097	19.027387027903636	31.147123517305374
25-29	31.093813789429287	19.00666928834841	18.948413953380232	30.951102968842072
30-34	31.253010284826154	18.775818921272982	18.7542101470302	31.21696064687066
35-39	31.082298878163055	18.832299503211498	18.90546517355095	31.179936445074492
40-44	31.211670575171745	18.901479571923442	18.8169994620993	31.06985039080551
45-49	31.329065	18.670165	18.6923	31.30847
50-54	31.13600023840015	18.764437196884177	18.84092724660271	31.258635318112958
55-59	31.253750000000004	18.84414	18.805205	31.096905000000003
60-64	31.380715277509964	18.63004159139234	18.654931700510165	31.334311430587537
65-69	31.168151642496607	18.745627826813475	18.827181705041752	31.25903882564816
70-74	31.31341	18.83077	18.74843	31.107390000000002
75-79	31.39815	18.62576	18.612714999999998	31.363374999999998
80-84	31.225502912199598	18.72780549094779	18.771686991695116	31.2750046051575
85-89	31.323666263767898	18.80635424630545	18.744271322185718	31.12570816774094
90-94	31.45593531806698	18.596861185692358	18.599457612039174	31.347745884201483
95-99	31.287737828581395	18.67202148943431	18.744771761772764	31.295468920211533
100-101	31.58692835032867	18.823481029042846	18.63982962870092	30.949760991927562
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.10756788804339
2	56.179100000000005
3	58.601925
4	60.25772499999999
5	59.289899999999996
6	63.085424999999994
7	61.947125
8	60.506800000000005
9	62.800675
10-14	61.518905000000004
15-19	62.19352500000001
20-24	62.076718238733264
25-29	62.04491675827136
30-34	62.469970931696814
35-39	62.26223532323755
40-44	62.28152096597726
45-49	62.637535
50-54	62.39463555651311
55-59	62.350654999999996
60-64	62.7150267080975
65-69	62.427190468144765
70-74	62.4208
75-79	62.761525
80-84	62.50050751735709
85-89	62.44937443150883
90-94	62.80368120226847
95-99	62.58320674879293
100-101	62.53668934225624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	2.0
4	2.5
5	2.5
6	1.5
7	3.5
8	6.0
9	4.5
10	7.5
11	14.0
12	18.0
13	32.0
14	45.0
15	52.5
16	69.0
17	103.5
18	152.5
19	205.5
20	280.5
21	360.0
22	476.5
23	619.5
24	809.0
25	1118.0
26	1443.5
27	1806.5
28	2243.0
29	2703.0
30	3285.0
31	4033.0
32	4711.5
33	5450.0
34	6205.5
35	6835.0
36	7552.0
37	8432.0
38	9311.0
39	10151.5
40	11054.5
41	12096.5
42	13421.5
43	14998.0
44	17095.0
45	19882.0
46	23454.0
47	27956.5
48	33654.0
49	40178.0
50	47892.5
51	57048.0
52	66944.0
53	77827.0
54	89325.0
55	100740.0
56	112601.0
57	124443.0
58	135904.0
59	147347.5
60	158568.0
61	169322.0
62	179377.5
63	187569.0
64	194026.0
65	198540.0
66	199491.0
67	197294.0
68	190793.0
69	179823.0
70	165894.5
71	148921.0
72	129964.5
73	110067.0
74	90190.0
75	71484.5
76	54799.5
77	40559.0
78	28652.5
79	19498.5
80	12788.0
81	7908.0
82	4748.5
83	2844.5
84	1640.0
85	984.0
86	608.0
87	400.5
88	267.0
89	188.5
90	127.0
91	86.0
92	59.5
93	41.5
94	26.5
95	17.5
96	9.0
97	3.0
98	2.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021975
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.001745
25-29	5.75E-4
30-34	0.040604999999999995
35-39	0.00775
40-44	1.3000000000000002E-4
45-49	0.0
50-54	6.500000000000001E-5
55-59	0.0
60-64	0.040615
65-69	0.03541
70-74	0.0
75-79	0.0
80-84	0.00342
85-89	0.036925
90-94	0.054935
95-99	0.014119999999999999
100-101	7.624999999999999E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1384916904985672
#Duplication Level	Relative count
1	100.0
2	0.1266678348812176
3	0.004005307031817158
4	0.0015019901369314342
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.006633789771447E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	282505	3.0775037	3.6657522	9
>>END_MODULE