##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_011.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2592787	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34546648066347	34.0	31.0	34.0	31.0	34.0
2	32.50412664056091	34.0	31.0	34.0	31.0	34.0
3	32.555192925604764	34.0	31.0	34.0	31.0	34.0
4	35.98270239707311	37.0	37.0	37.0	35.0	37.0
5	35.99231830458885	37.0	37.0	37.0	35.0	37.0
6	35.96787086636889	37.0	37.0	37.0	35.0	37.0
7	35.94490600269131	37.0	37.0	37.0	35.0	37.0
8	35.96186497386789	37.0	37.0	37.0	35.0	37.0
9	37.68394742799929	39.0	38.0	39.0	35.0	39.0
10-14	37.85260331835974	39.4	38.2	39.4	35.0	39.4
15-19	38.97215961048864	41.0	39.0	41.0	35.6	41.0
20-24	38.7588177509375	40.4	38.6	41.0	34.8	41.0
25-29	38.30760174283502	40.0	38.0	41.0	33.6	41.0
30-34	37.65861507327829	39.8	36.6	41.0	32.8	41.0
35-39	36.64160835425355	38.4	35.0	40.6	31.4	41.0
40-44	35.698603163314225	36.4	35.0	39.8	31.0	41.0
45-49	34.765553283011684	35.0	34.4	38.4	29.8	40.8
50-54	33.484094374123295	34.8	33.2	35.8	27.8	39.2
55-59	33.612622633482815	35.0	34.0	35.0	29.4	38.0
60-64	33.22414506089393	35.0	34.0	35.0	28.8	36.2
65-69	32.935577430772376	35.0	33.4	35.0	29.0	35.0
70-74	32.62959880622666	35.0	33.0	35.0	27.4	35.0
75-79	32.39539746226743	35.0	33.0	35.0	27.0	35.0
80-84	32.13376555806551	35.0	33.0	35.0	27.0	35.0
85-89	31.81992041768182	35.0	33.0	35.0	25.4	35.0
90-94	31.413253846150877	35.0	33.0	35.0	24.0	35.0
95-99	30.84557188847368	35.0	32.4	35.0	22.2	35.0
100-101	29.648892485190647	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3377.0
3	874.0
4	1161.0
5	1443.0
6	1988.0
7	2764.0
8	3287.0
9	3824.0
10	4047.0
11	4094.0
12	4043.0
13	4069.0
14	3979.0
15	4121.0
16	4548.0
17	4994.0
18	5612.0
19	6242.0
20	7404.0
21	8692.0
22	10694.0
23	12995.0
24	15909.0
25	19323.0
26	23162.0
27	27806.0
28	34105.0
29	41809.0
30	51234.0
31	64317.0
32	83273.0
33	114271.0
34	183873.0
35	430013.0
36	875414.0
37	441148.0
38	73297.0
39	9554.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.24920319816313	22.743513519977718	13.572364883162734	26.434918398696418
2	31.435976602340077	24.08196895844616	19.141134302012503	25.340920137201262
3	28.69664739991421	19.631025746265202	20.92582248871306	30.74650436510753
4	30.96472684910158	19.34048002962574	19.460641736809215	30.23415138446346
5	31.413954274195135	20.626873466944243	19.82138152552814	28.137790733332483
6	29.727963710493988	19.060476724069765	17.582396812324856	33.629162753111395
7	32.87471706148194	18.712747392799667	19.035416882036817	29.37711866368157
8	28.62276114350785	19.517161914394684	19.826072130226795	32.03400481187067
9	30.693579983442472	17.89791117417272	19.15847470115818	32.25003414122663
10-14	30.892882903524182	19.25738636845788	19.106685088712023	30.743045639305915
15-19	31.067554099695123	18.810585475446974	18.94862684172478	31.173233583133126
20-24	30.974566550502114	18.89386702689043	18.961393555004037	31.17017286760342
25-29	31.145514518498302	19.012708020231564	18.862581162187993	30.979196299082133
30-34	31.29140347491032	18.75075503460546	18.72127107790476	31.23657041257945
35-39	31.094666849026414	18.84817602066497	18.849417980038705	31.20773915026991
40-44	31.25696164177806	18.895916783041148	18.77509662218827	31.07202495299253
45-49	31.363621321798067	18.696426468050635	18.62272146040668	31.317230749744617
50-54	31.156000581318033	18.80416494004309	18.804712625554977	31.235121853083903
55-59	31.27852588589152	18.853574112997684	18.74994600292016	31.117953998190632
60-64	31.387661230945696	18.66633857698299	18.615042423461702	31.330957768609608
65-69	31.181607862606185	18.774014867812355	18.776645260464413	31.26773200911705
70-74	31.293885691342943	18.838223116669436	18.744694415700174	31.123196776287447
75-79	31.380882851042635	18.666691453387095	18.627714078391808	31.324711617178462
80-84	31.20777648881351	18.75434524047823	18.789890107889626	31.247988162818636
85-89	31.316903393915506	18.852616894484584	18.728163940963913	31.102315770635997
90-94	31.393454225125318	18.674592243790176	18.591230209037608	31.34072332204689
95-99	31.233464222089975	18.737698083182305	18.770990443873718	31.257847250854002
100-101	31.424139352750537	18.936630737503698	18.727589269770327	30.91164063997544
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.68412159685954
2	56.776896739541336
3	59.44315176502174
4	61.19887823356505
5	59.55174500752762
6	63.35712646360538
7	62.25183572516352
8	60.656765955378525
9	62.943614124669104
10-14	61.6359285428301
15-19	62.24078768282825
20-24	62.14473941810553
25-29	62.12471081758044
30-34	62.52797388748977
35-39	62.302405999296326
40-44	62.32898659477058
45-49	62.68085207154268
50-54	62.391122434401936
55-59	62.396479884082154
60-64	62.71861899955531
65-69	62.44933987172323
70-74	62.417082467630394
75-79	62.70559446822109
80-84	62.45576465163214
85-89	62.41921916455151
90-94	62.73417754717221
95-99	62.49131147294398
100-101	62.33577999272597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	73.0
1	44.5
2	14.0
3	12.0
4	11.5
5	11.0
6	15.5
7	16.5
8	14.0
9	18.5
10	25.5
11	28.0
12	33.0
13	38.5
14	41.5
15	54.0
16	77.5
17	103.5
18	122.5
19	148.5
20	194.0
21	254.5
22	324.5
23	438.5
24	576.5
25	733.0
26	928.0
27	1161.5
28	1454.0
29	1750.5
30	2080.5
31	2553.5
32	3036.0
33	3458.0
34	3835.5
35	4316.5
36	4943.5
37	5400.5
38	5885.5
39	6393.0
40	6961.5
41	7750.5
42	8500.0
43	9453.0
44	10820.0
45	12670.0
46	15011.5
47	17719.0
48	21213.5
49	25546.0
50	30504.0
51	36153.5
52	42795.5
53	49979.5
54	57102.0
55	64502.0
56	72187.0
57	79990.0
58	87683.0
59	95173.5
60	102461.5
61	108978.0
62	115493.0
63	121616.0
64	126479.5
65	129504.5
66	130360.0
67	128891.5
68	124750.0
69	118365.5
70	109401.0
71	98020.5
72	85255.0
73	72307.5
74	59359.5
75	46873.5
76	35845.5
77	26507.0
78	18624.0
79	12383.5
80	7876.5
81	4780.0
82	2736.0
83	1507.0
84	828.0
85	458.0
86	257.5
87	148.5
88	109.5
89	85.5
90	54.5
91	40.0
92	36.0
93	25.5
94	19.5
95	16.5
96	10.0
97	8.5
98	7.0
99	4.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021174126528712155
2	0.015080297764529058
3	0.015851668494172487
4	0.01724013580753066
5	0.01288189118504528
6	0.011647698017615794
7	0.013537556305242195
8	0.01727870434401283
9	0.023642512863571132
10-14	0.009295017292203332
15-19	0.008901618220085183
20-24	0.0010876327287972364
25-29	0.0017895800927727576
30-34	0.007351163053501889
35-39	0.004227111598445996
40-44	3.008345845609377E-4
45-49	7.250884858648242E-4
50-54	0.0022446888232623813
55-59	0.0020904146773336957
60-64	0.0
65-69	7.019473639755213E-4
70-74	0.0
75-79	3.08548291857372E-5
80-84	2.931208772645034E-4
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2592787.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1684258926072418
#Duplication Level	Relative count
1	100.0
2	0.12165145606279819
3	0.0020024931039143734
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010012465519571867
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	199465	3.2471583	4.3353605	1
TTTTT	182645	3.1012642	3.5990496	10-14
>>END_MODULE