##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_010.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64455675	34.0	31.0	34.0	31.0	34.0
2	32.78292725	34.0	31.0	34.0	31.0	34.0
3	32.8317415	34.0	31.0	34.0	31.0	34.0
4	36.189305	37.0	37.0	37.0	35.0	37.0
5	36.20235475	37.0	37.0	37.0	35.0	37.0
6	36.20394325	37.0	37.0	37.0	35.0	37.0
7	36.17766425	37.0	37.0	37.0	35.0	37.0
8	36.1741215	37.0	37.0	37.0	35.0	37.0
9	37.93655225	39.0	39.0	39.0	37.0	39.0
10-14	38.189729	39.4	39.2	39.4	35.8	39.4
15-19	39.3518298	41.0	40.0	41.0	36.6	41.0
20-24	39.13765005	41.0	39.0	41.0	35.8	41.0
25-29	38.752652999999995	40.4	38.6	41.0	35.0	41.0
30-34	38.0633155	40.0	37.2	41.0	33.8	41.0
35-39	37.041621199999994	38.8	35.2	40.8	32.6	41.0
40-44	36.048422599999995	37.0	35.0	40.0	31.8	41.0
45-49	35.074975099999996	35.0	35.0	38.4	30.8	41.0
50-54	33.76950865	34.8	33.4	36.0	29.0	39.2
55-59	33.89244155	35.0	35.0	35.0	31.0	38.2
60-64	33.5193615	35.0	34.4	35.0	30.4	36.4
65-69	33.20453935	35.0	34.0	35.0	29.8	35.0
70-74	32.9275215	35.0	34.0	35.0	29.0	35.0
75-79	32.6397348	35.0	33.0	35.0	28.6	35.0
80-84	32.362287949999995	35.0	33.0	35.0	27.0	35.0
85-89	32.033912900000004	35.0	33.0	35.0	27.0	35.0
90-94	31.63089025	35.0	33.0	35.0	24.6	35.0
95-99	31.078517249999997	35.0	32.8	35.0	23.6	35.0
100-101	30.009199875	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7590.0
3	1189.0
4	1604.0
5	2045.0
6	2686.0
7	3251.0
8	3996.0
9	4708.0
10	4934.0
11	5318.0
12	5369.0
13	5381.0
14	5299.0
15	5385.0
16	6033.0
17	6630.0
18	7227.0
19	8199.0
20	9617.0
21	11696.0
22	13554.0
23	16656.0
24	19974.0
25	24146.0
26	29849.0
27	36441.0
28	43953.0
29	54084.0
30	66892.0
31	85638.0
32	112921.0
33	159573.0
34	262286.0
35	646443.0
36	1410927.0
37	761835.0
38	128880.0
39	17743.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.2312545765866	22.706651497716898	13.558308278372586	26.50378564732392
2	31.45810704333333	24.05258460399782	19.117254438912145	25.372053913756705
3	28.709420259756378	19.605160897722364	20.905994574271478	30.779424268249777
4	31.003693287025452	19.344116263474493	19.386028565423228	30.26616188407683
5	31.449931746489245	20.605379617620216	19.82431726473555	28.120371371154985
6	29.70969548962569	19.084895173584	17.536911352957173	33.668497983833134
7	32.95049469277706	18.68127963834371	19.00236768972981	29.365857979149418
8	28.58622461146632	19.48137287947705	19.850945000091336	32.08145750896529
9	30.725016705165714	17.853104658176022	19.15765922463005	32.264219412028226
10-14	30.86608674491026	19.23561978443529	19.11417833096826	30.78411513968619
15-19	31.075726492545	18.792565557788297	18.94393088767306	31.187777061993643
20-24	30.97312731565563	18.864483558463636	18.989028905424465	31.17336022045627
25-29	31.119201684314486	18.975433712430142	18.885167565351544	31.02019703790383
30-34	31.268895235388648	18.734932344400125	18.72711707690984	31.269055343301382
35-39	31.083969748238832	18.821666088031865	18.846699130246858	31.24766503348245
40-44	31.254967107502175	18.869919809611154	18.76223944550139	31.112873637385285
45-49	31.365245145345185	18.660410108955432	18.614111115084533	31.360233630614854
50-54	31.173284218026104	18.765196598782982	18.77991738552425	31.281601797666664
55-59	31.258804208703744	18.837068912954607	18.747240537221604	31.156886341120043
60-64	31.384269999999997	18.634800000000002	18.596799999999998	31.38413
65-69	31.186560000000004	18.746365	18.77124	31.295835
70-74	31.29904	18.84978	18.722755	31.128425
75-79	31.393239361883257	18.649810482180754	18.60196787715091	31.35498227878508
80-84	31.192888972810234	18.759572729894412	18.768338798916528	31.27919949837883
85-89	31.34307	18.82523	18.69441	31.13729
90-94	31.404500000000002	18.637065	18.587619999999998	31.370815
95-99	31.23230301734492	18.731000463419186	18.74837118256696	31.288325336668937
100-101	31.446970387351943	18.906269512887082	18.678230859072688	30.968529240688287
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.73504022391051
2	56.83016095709004
3	59.488844528006155
4	61.269855171102286
5	59.57030311764423
6	63.378193473458836
7	62.31635267192648
8	60.66768212043162
9	62.98923611719394
10-14	61.65020188459644
15-19	62.263503554538644
20-24	62.14648753611189
25-29	62.13939872221831
30-34	62.53795057869004
35-39	62.33163478172128
40-44	62.36784074488746
45-49	62.72547877596003
50-54	62.454886015692765
55-59	62.415690549823786
60-64	62.7684
65-69	62.482395000000004
70-74	62.427465000000005
75-79	62.74822164066833
80-84	62.47208847118906
85-89	62.480360000000005
90-94	62.775315
95-99	62.52062835401385
100-101	62.41549962804023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	72.5
2	26.0
3	19.0
4	17.5
5	14.0
6	18.0
7	22.5
8	23.0
9	30.5
10	46.5
11	54.0
12	58.0
13	74.5
14	105.0
15	145.0
16	183.0
17	233.0
18	268.5
19	323.0
20	409.0
21	480.0
22	581.0
23	756.5
24	973.0
25	1214.5
26	1524.5
27	1892.0
28	2303.5
29	2800.5
30	3351.5
31	3885.0
32	4626.5
33	5338.0
34	6101.5
35	6982.0
36	7651.5
37	8358.5
38	9114.5
39	9988.0
40	10942.5
41	11916.5
42	13069.5
43	14739.0
44	17046.0
45	19640.0
46	22943.5
47	27402.5
48	32791.5
49	39244.0
50	47097.0
51	56008.0
52	65852.5
53	76609.5
54	87867.0
55	99198.5
56	110843.0
57	122883.5
58	134462.0
59	145805.0
60	157134.0
61	167944.0
62	178390.0
63	187362.0
64	194295.0
65	198626.5
66	200195.0
67	197879.5
68	191305.0
69	181458.5
70	167797.5
71	151296.5
72	132438.5
73	112367.0
74	92359.0
75	73166.5
76	56150.0
77	41405.5
78	29211.5
79	19919.0
80	12878.5
81	7862.5
82	4644.5
83	2747.0
84	1575.5
85	925.0
86	572.5
87	374.5
88	288.5
89	216.5
90	164.5
91	127.5
92	107.5
93	82.5
94	64.0
95	55.5
96	40.0
97	35.0
98	27.0
99	17.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10295
2	0.086725
3	0.08715
4	0.089
5	0.0784
6	0.076475
7	0.081925
8	0.093925
9	0.10575000000000001
10-14	0.062955
15-19	0.06298000000000001
20-24	0.03641
25-29	0.040045
30-34	0.0674
35-39	0.0521
40-44	0.023555
45-49	0.030225000000000002
50-54	0.039310000000000005
55-59	0.037155
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.005445
80-84	0.012195
85-89	0.0
90-94	0.0
95-99	0.00414
100-101	0.0059875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2662829793718061
#Duplication Level	Relative count
1	100.0
2	0.1733206431899013
3	0.0010018534288433602
4	0.0020037068576867203
5	5.009267144216801E-4
6	0.0
7	0.0010018534288433602
8	0.0
9	0.0
10++	0.007513900716325202
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	314685	3.340219	4.347622	1
TTTTT	293895	3.251003	3.7213652	10-14
>>END_MODULE