##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58043425	34.0	31.0	34.0	31.0	34.0
2	32.7580115	34.0	31.0	34.0	31.0	34.0
3	32.81190625	34.0	31.0	34.0	31.0	34.0
4	36.15788575	37.0	37.0	37.0	35.0	37.0
5	36.18643875	37.0	37.0	37.0	35.0	37.0
6	36.1901455	37.0	37.0	37.0	35.0	37.0
7	36.17553825	37.0	37.0	37.0	35.0	37.0
8	36.13098175	37.0	37.0	37.0	35.0	37.0
9	37.887444	39.0	39.0	39.0	35.0	39.0
10-14	38.15295875	39.4	38.6	39.4	35.4	39.4
15-19	39.2950774	41.0	39.6	41.0	36.2	41.0
20-24	39.093478250000004	41.0	39.0	41.0	35.8	41.0
25-29	38.60835515	40.0	38.2	41.0	35.0	41.0
30-34	38.006944100000005	40.0	37.2	41.0	33.6	41.0
35-39	36.98845535	38.6	35.0	40.8	32.6	41.0
40-44	36.0202235	36.8	35.0	40.0	31.4	41.0
45-49	35.081840500000006	35.0	35.0	38.4	31.0	41.0
50-54	33.74350595	34.8	33.4	36.0	28.8	39.2
55-59	33.8920413	35.0	34.8	35.0	30.8	38.0
60-64	33.51310545	35.0	34.0	35.0	30.0	36.2
65-69	33.2099798	35.0	34.0	35.0	29.8	35.0
70-74	32.93915325	35.0	33.8	35.0	29.0	35.0
75-79	32.661109599999996	35.0	33.0	35.0	28.6	35.0
80-84	32.3579086	35.0	33.0	35.0	27.0	35.0
85-89	32.0393573	35.0	33.0	35.0	27.0	35.0
90-94	31.637423650000006	35.0	33.0	35.0	25.0	35.0
95-99	31.05573185	35.0	32.6	35.0	23.6	35.0
100-101	29.918015375000003	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6679.0
3	1213.0
4	1669.0
5	1976.0
6	2642.0
7	3274.0
8	3928.0
9	4467.0
10	4887.0
11	4967.0
12	5130.0
13	5147.0
14	5268.0
15	5505.0
16	5845.0
17	6448.0
18	7210.0
19	8131.0
20	9393.0
21	11572.0
22	13767.0
23	16679.0
24	20282.0
25	24215.0
26	29885.0
27	36267.0
28	44681.0
29	54925.0
30	68501.0
31	88336.0
32	117323.0
33	166133.0
34	275458.0
35	667548.0
36	1400327.0
37	731946.0
38	122053.0
39	16277.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.23049044321101	22.756057537409678	13.552233921008423	26.461218098370892
2	31.411439933804548	24.054251449151526	19.13062668250327	25.40368193454066
3	28.72008397550781	19.583819904957174	20.941367243748118	30.754728875786896
4	31.003849066948952	19.343387399033283	19.41685184944315	30.235911684574617
5	31.428919435966478	20.613324802983687	19.810378130757954	28.14737763029188
6	29.719987475190475	19.055592347461424	17.58382718964082	33.64059298770728
7	32.88914929717283	18.72405333610865	19.01613702990787	29.37066033681065
8	28.595614396647317	19.51956618959635	19.84790708953571	32.03691232422062
9	30.7053054066107	17.85899297427404	19.16904035800821	32.266661261107046
10-14	30.87637157291535	19.241304544972756	19.109850234624435	30.77247364748746
15-19	31.06767281091813	18.79018562622054	18.947282481537005	31.19485908132432
20-24	30.9643413740039	18.875225614392274	18.988320360734985	31.172112650868844
25-29	31.162363960801525	18.996725492315132	18.86685440770779	30.974056139175556
30-34	31.263444272748565	18.723125834741598	18.712371917089396	31.30105797542044
35-39	31.095980712066968	18.826349339379092	18.863552697113406	31.214117251440538
40-44	31.233958300068743	18.877643200339485	18.781861184485592	31.10653731510618
45-49	31.344294947382224	18.661158073653937	18.646206645044934	31.348340333918905
50-54	31.167713587033084	18.7679598608316	18.801592207817798	31.262734344317515
55-59	31.25952685061003	18.84547260155152	18.768475170316247	31.126525377522203
60-64	31.371325	18.63367	18.62258	31.372425
65-69	31.171767292252493	18.752749705785543	18.803445042909537	31.27203795905243
70-74	31.295	18.84001	18.727545	31.137445000000003
75-79	31.387027119731375	18.627555639112593	18.608475300436584	31.376941940719448
80-84	31.212691171805716	18.744557306240104	18.763227970915764	31.27952355103842
85-89	31.314700000000002	18.836135000000002	18.73104	31.118125000000003
90-94	31.406715000000002	18.634855	18.59787	31.36056
95-99	31.214555704157842	18.72389834803456	18.777934180186374	31.283611767621224
100-101	31.425850292175728	18.93011445648265	18.688496187846486	30.95553906349514
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.6917085415819
2	56.81512186834521
3	59.4748128512947
4	61.23976075152356
5	59.576297066258356
6	63.36058046289775
7	62.25980963398348
8	60.63252672086794
9	62.97196666771775
10-14	61.64884522040281
15-19	62.26253189224246
20-24	62.13645402487275
25-29	62.13642009997709
30-34	62.564502248169006
35-39	62.3100979635075
40-44	62.34049561517493
45-49	62.69263528130112
50-54	62.430447931350606
55-59	62.38605222813223
60-64	62.74375
65-69	62.443805251304916
70-74	62.432445
75-79	62.76396906045082
80-84	62.49221472284413
85-89	62.432825
90-94	62.767275
95-99	62.49816747177906
100-101	62.38138935567087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	107.5
2	48.0
3	41.5
4	42.5
5	43.0
6	45.0
7	43.5
8	41.5
9	51.0
10	56.0
11	52.0
12	57.5
13	74.0
14	98.0
15	128.0
16	159.5
17	195.5
18	225.0
19	269.5
20	348.5
21	442.0
22	582.0
23	718.0
24	871.0
25	1164.5
26	1477.0
27	1817.5
28	2256.5
29	2745.0
30	3342.0
31	3952.5
32	4624.0
33	5323.5
34	6093.0
35	6832.5
36	7507.5
37	8309.5
38	9136.5
39	9968.5
40	10704.5
41	11703.5
42	13035.5
43	14630.0
44	16909.0
45	19655.0
46	22906.0
47	27217.5
48	32702.0
49	39213.0
50	47066.5
51	55974.0
52	65876.5
53	76633.0
54	87980.5
55	99501.5
56	111406.0
57	123432.5
58	134724.5
59	146020.0
60	157172.5
61	167916.5
62	178216.5
63	187349.0
64	194539.0
65	199312.0
66	200516.5
67	198604.5
68	192853.5
69	182715.5
70	168750.5
71	151220.0
72	132057.0
73	111691.5
74	91655.0
75	72680.0
76	55372.0
77	40817.5
78	28756.0
79	19421.0
80	12589.5
81	7790.0
82	4552.5
83	2579.5
84	1445.5
85	839.0
86	518.5
87	339.5
88	238.5
89	184.5
90	153.5
91	124.5
92	95.0
93	70.5
94	59.0
95	47.5
96	33.0
97	23.0
98	15.0
99	12.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08935
2	0.08377499999999999
3	0.0661
4	0.053700000000000005
5	0.043175
6	0.038400000000000004
7	0.07145
8	0.10382499999999999
9	0.10285
10-14	0.041315
15-19	0.052105
20-24	0.01746
25-29	0.020085
30-34	0.036430000000000004
35-39	0.022465000000000002
40-44	0.012545
45-49	0.009555000000000001
50-54	0.021845
55-59	0.016145
60-64	0.0
65-69	6.65E-4
70-74	0.0
75-79	0.001775
80-84	0.00356
85-89	0.0
90-94	0.0
95-99	0.0015400000000000001
100-101	0.0023875000000000003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.22190458103015553
#Duplication Level	Relative count
1	100.0
2	0.17581470832790697
3	0.0015026898147684354
4	0.0010017932098456237
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010017932098456237
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	310460	3.2995427	3.9816432	1
TTTTT	298140	3.2936623	3.6514316	9
>>END_MODULE