##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67431225	34.0	31.0	34.0	31.0	34.0
2	32.83045425	34.0	31.0	34.0	31.0	34.0
3	32.88843425	34.0	33.0	34.0	31.0	34.0
4	36.23284875	37.0	37.0	37.0	35.0	37.0
5	36.24494825	37.0	37.0	37.0	35.0	37.0
6	36.2422975	37.0	37.0	37.0	35.0	37.0
7	36.230321	37.0	37.0	37.0	35.0	37.0
8	36.19485725	37.0	37.0	37.0	35.0	37.0
9	37.9667735	39.0	39.0	39.0	37.0	39.0
10-14	38.2211702	39.4	39.2	39.4	37.0	39.4
15-19	39.168245850000005	41.0	39.8	41.0	36.4	41.0
20-24	38.55326974999999	40.6	38.4	41.0	34.2	41.0
25-29	38.693602600000006	40.0	38.4	41.0	35.0	41.0
30-34	38.11377125	40.0	37.4	41.0	34.2	41.0
35-39	36.942748699999996	38.6	35.2	40.8	32.2	41.0
40-44	36.05202945	37.0	35.0	40.0	32.0	41.0
45-49	35.0757538	35.0	35.0	38.6	30.8	41.0
50-54	33.79376755	34.8	33.6	36.2	29.2	39.2
55-59	33.954647699999995	35.0	35.0	35.0	31.0	38.2
60-64	33.552015600000004	35.0	34.6	35.0	30.6	36.4
65-69	33.2243379	35.0	34.0	35.0	30.0	35.0
70-74	32.93842495	35.0	34.0	35.0	29.0	35.0
75-79	32.4856104	35.0	33.0	35.0	28.2	35.0
80-84	32.27616335	35.0	33.0	35.0	27.0	35.0
85-89	31.94589155	35.0	33.0	35.0	26.6	35.0
90-94	31.13128615	35.0	32.2	35.0	21.8	35.0
95-99	30.68659655	35.0	31.8	35.0	20.8	35.0
100-101	28.962308	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9711.0
3	1341.0
4	1875.0
5	2213.0
6	2792.0
7	3331.0
8	3942.0
9	4448.0
10	4767.0
11	5076.0
12	5237.0
13	5290.0
14	5254.0
15	5479.0
16	5930.0
17	6509.0
18	7383.0
19	8248.0
20	9593.0
21	11562.0
22	13663.0
23	16263.0
24	19896.0
25	24107.0
26	28970.0
27	35692.0
28	42972.0
29	53738.0
30	66854.0
31	87349.0
32	117814.0
33	173155.0
34	309059.0
35	753173.0
36	1353768.0
37	666175.0
38	112740.0
39	14583.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.23956272284374	22.706994851614493	13.563501262802088	26.489941162739672
2	31.423080698703604	24.087103553419492	19.108845384080634	25.380970363796273
3	28.695554641454844	19.62803166883272	20.935273574680956	30.741140115031477
4	30.9565160796133	19.38347505888891	19.398018929561108	30.26198993193669
5	31.409135826943118	20.631126770011406	19.83914584903979	28.120591554005685
6	29.67966778063792	19.103614342050403	17.58846661865095	33.628251258660725
7	32.86486919594567	18.737004727374703	19.05762990264813	29.34049617403149
8	28.616890457788386	19.481763839599843	19.821915656677874	32.0794300459339
9	30.723015302454552	17.85978553017532	19.1415946715696	32.27560449580053
10-14	30.866552416435432	19.25032808275789	19.121897929285883	30.761221571520792
15-19	31.31665284435699	18.838791818994927	18.845915740281555	30.99863959636653
20-24	31.098087387263423	19.00821835557835	18.847959170909274	31.045735086248953
25-29	31.118120210247625	18.987472434758196	18.88944267187744	31.004964683116743
30-34	31.269137853405272	18.752408654318742	18.7040503830924	31.274403109183584
35-39	31.202922080846278	18.865502581773853	18.818648575135832	31.112926762244037
40-44	31.23803624717847	18.882575042146282	18.781098752760244	31.09828995791501
45-49	31.357943080666246	18.704717794973416	18.61353878064212	31.323800343718222
50-54	31.145448282377508	18.777201260505212	18.802596109118888	31.274754347998396
55-59	31.270346409170546	18.83917509742111	18.757021459310785	31.13345703409756
60-64	31.37167333184466	18.637976684560364	18.619516653178312	31.370833330416666
65-69	31.162515000000003	18.752355	18.78669	31.29844
70-74	31.290705000000003	18.824360000000002	18.741045	31.143890000000003
75-79	31.454729076139355	18.669305510328016	18.57969497212872	31.296270441403912
80-84	31.1876046309743	18.75572197710319	18.766679566381537	31.289993825540975
85-89	31.322121566106077	18.817905940895297	18.719115935955795	31.140856557042827
90-94	31.47655856754332	18.717175647004474	18.53374402363461	31.27252176181759
95-99	31.24759248832855	18.713619788904577	18.745964144072	31.292823578694872
100-101	31.598630060417843	19.056997994658342	18.666520947923765	30.67785099700005
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.72950388558342
2	56.80405106249987
3	59.43669475648633
4	61.21850601154999
5	59.529727380948806
6	63.307919039298646
7	62.20536536997716
8	60.696320503722276
9	62.998619798255085
10-14	61.627773987956225
15-19	62.31529244072351
20-24	62.14382247351238
25-29	62.123084893364364
30-34	62.543540962588864
35-39	62.315848843090315
40-44	62.33632620509347
45-49	62.681743424384464
50-54	62.4202026303759
55-59	62.40380344326811
60-64	62.74250666226132
65-69	62.460955
70-74	62.434595
75-79	62.75099951754326
80-84	62.477598456515274
85-89	62.46297812314891
90-94	62.749080329360915
95-99	62.54041606702343
100-101	62.2764810574179
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	62.5
2	27.5
3	28.5
4	33.0
5	31.0
6	32.0
7	36.5
8	46.0
9	46.0
10	49.0
11	70.0
12	88.5
13	104.5
14	146.0
15	208.5
16	245.0
17	268.0
18	335.5
19	407.0
20	450.5
21	544.0
22	665.0
23	815.5
24	990.5
25	1250.5
26	1570.0
27	1900.0
28	2343.5
29	2877.5
30	3407.0
31	3991.5
32	4690.0
33	5409.5
34	6120.5
35	6859.0
36	7606.0
37	8368.0
38	9108.0
39	9920.0
40	10870.5
41	11943.5
42	13305.0
43	14974.0
44	16974.0
45	19567.5
46	22999.5
47	27499.5
48	32884.0
49	39328.0
50	47192.5
51	56049.0
52	65744.5
53	76580.5
54	88289.5
55	99641.0
56	111340.5
57	123054.5
58	134242.5
59	146146.5
60	157841.5
61	168343.5
62	178530.5
63	187296.5
64	194105.0
65	199088.5
66	200480.0
67	198172.0
68	191874.5
69	181544.0
70	167404.0
71	150506.5
72	131693.0
73	111300.0
74	91227.5
75	72343.5
76	55267.0
77	40626.5
78	28877.5
79	19815.0
80	12893.0
81	7990.0
82	4733.0
83	2800.5
84	1629.5
85	990.5
86	682.0
87	486.0
88	354.0
89	269.0
90	213.0
91	173.5
92	144.5
93	111.5
94	92.0
95	83.5
96	67.0
97	47.5
98	33.5
99	23.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1823
2	0.1694
3	0.144725
4	0.12975
5	0.1207
6	0.10215
7	0.156
8	0.198975
9	0.19575
10-14	0.10523500000000001
15-19	0.12523
20-24	0.07125000000000001
25-29	0.07116499999999999
30-34	0.09982
35-39	0.07257999999999999
40-44	0.065325
45-49	0.037305
50-54	0.07816000000000001
55-59	0.06529
60-64	1.7E-4
65-69	0.0
70-74	0.0
75-79	0.01176
80-84	0.023629999999999998
85-89	4.9999999999999996E-6
90-94	8.85E-4
95-99	0.013465
100-101	0.0357375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.18842180495095673
#Duplication Level	Relative count
1	100.0
2	0.1462149359558551
3	5.007360820405997E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007360820405997E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	294835	3.2806048	3.7247012	10-14
AAAAA	309815	3.2736733	4.0937395	1
>>END_MODULE