##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.426978	34.0	31.0	34.0	31.0	34.0
2	32.59572075	34.0	31.0	34.0	31.0	34.0
3	32.64584675	34.0	31.0	34.0	31.0	34.0
4	36.04095925	37.0	37.0	37.0	35.0	37.0
5	36.034155	37.0	37.0	37.0	35.0	37.0
6	36.05404625	37.0	37.0	37.0	35.0	37.0
7	36.03718225	37.0	37.0	37.0	35.0	37.0
8	36.04724025	37.0	37.0	37.0	35.0	37.0
9	37.77127425	39.0	38.0	39.0	35.0	39.0
10-14	37.9977255	39.4	38.2	39.4	35.2	39.4
15-19	39.065057100000004	41.0	39.0	41.0	36.0	41.0
20-24	38.9085778	40.6	39.0	41.0	35.2	41.0
25-29	38.53096305	40.0	38.0	41.0	34.6	41.0
30-34	37.827011500000005	40.0	36.8	41.0	33.2	41.0
35-39	36.841950499999996	38.6	35.0	40.6	31.8	41.0
40-44	35.865070949999996	36.6	35.0	40.0	31.0	41.0
45-49	34.95206875	35.0	34.8	38.4	30.6	40.8
50-54	33.6590414	34.8	33.2	35.8	28.4	39.2
55-59	33.76971895	35.0	34.0	35.0	30.0	38.0
60-64	33.414033999999994	35.0	34.0	35.0	30.0	36.2
65-69	33.1175643	35.0	34.0	35.0	29.0	35.0
70-74	32.86253369999999	35.0	33.4	35.0	29.0	35.0
75-79	32.584570150000005	35.0	33.0	35.0	27.8	35.0
80-84	32.3006213	35.0	33.0	35.0	27.0	35.0
85-89	32.026103500000005	35.0	33.0	35.0	27.0	35.0
90-94	31.663834050000002	35.0	33.0	35.0	24.6	35.0
95-99	31.093298649999998	35.0	32.6	35.0	23.6	35.0
100-101	30.031969375	34.0	30.5	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7121.0
3	1213.0
4	1596.0
5	2055.0
6	2566.0
7	3470.0
8	4251.0
9	4810.0
10	5076.0
11	5380.0
12	5538.0
13	5358.0
14	5449.0
15	5534.0
16	5767.0
17	6596.0
18	7460.0
19	8421.0
20	9981.0
21	11910.0
22	14286.0
23	17850.0
24	21439.0
25	26624.0
26	32350.0
27	39452.0
28	48627.0
29	59938.0
30	74252.0
31	95138.0
32	124681.0
33	173470.0
34	277657.0
35	649215.0
36	1376827.0
37	722706.0
38	119865.0
39	16018.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.174779823418106	22.75596137890701	13.602328721921822	26.46693007575306
2	31.409108849041694	24.07553909210835	19.134394303008794	25.38095775584116
3	28.69586212763402	19.664251364862363	20.943018560126504	30.69686794737711
4	30.957539325519324	19.369765617277075	19.44937390745067	30.22332114975293
5	31.397871207743083	20.648263720553405	19.84369067412589	28.11017439757762
6	29.715324999999996	19.04665	17.6259	33.612125
7	32.89575496144522	18.700975251087968	19.025883942395456	29.37738584507136
8	28.578704189856136	19.549561712745582	19.858863003355285	32.01287109404299
9	30.716840635263555	17.86779386815393	19.190380280475136	32.22498521610738
10-14	30.878407825190063	19.25745116906437	19.121747179367073	30.742393826378496
15-19	31.06851694249787	18.817530403666044	18.93391556078601	31.18003709305008
20-24	30.97271	18.87221	18.99329	31.16179
25-29	31.115579999999998	18.99855	18.89497	30.9909
30-34	31.269794196607442	18.790455613972796	18.713982681235823	31.22576750818394
35-39	31.084659999999996	18.83963	18.85915	31.216559999999998
40-44	31.232490000000002	18.888825	18.79169	31.086995
45-49	31.346345	18.685385	18.647949999999998	31.320320000000002
50-54	31.14497759475354	18.775351528866818	18.809946909416002	31.269723966963635
55-59	31.268738126873814	18.845376884537686	18.748791874879185	31.137093113709312
60-64	31.37895	18.647540000000003	18.63657	31.336940000000002
65-69	31.165558239061863	18.761564089675613	18.809194201606374	31.263683469656158
70-74	31.278270000000003	18.855615	18.73789	31.128224999999997
75-79	31.369235	18.637535	18.633765	31.359464999999997
80-84	31.170495	18.749015	18.791764999999998	31.288725
85-89	31.303985	18.845064999999998	18.72887	31.12208
90-94	31.393304999999998	18.636705	18.618705	31.351285
95-99	31.21248	18.75173	18.77153	31.26426
100-101	31.419262500000002	18.936475	18.691575	30.952687499999996
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.641709899171175
2	56.79006660488286
3	59.39273007501114
4	61.18086047527226
5	59.5080456053207
6	63.32745
7	62.27314080651657
8	60.59157528389913
9	62.941825851370936
10-14	61.620801651568556
15-19	62.24855403554794
20-24	62.1345
25-29	62.10648
30-34	62.49556170479138
35-39	62.30122
40-44	62.319485
45-49	62.666665
50-54	62.41470156171718
55-59	62.40583124058312
60-64	62.715889999999995
65-69	62.42924170871802
70-74	62.40649499999999
75-79	62.7287
80-84	62.45922
85-89	62.426064999999994
90-94	62.74459
95-99	62.47674
100-101	62.37195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	96.5
2	55.5
3	51.0
4	49.0
5	46.0
6	53.0
7	65.0
8	71.0
9	71.0
10	77.0
11	88.0
12	93.5
13	103.5
14	118.5
15	138.0
16	167.0
17	184.5
18	224.5
19	264.5
20	344.5
21	447.5
22	568.5
23	731.0
24	886.5
25	1130.0
26	1440.0
27	1817.5
28	2292.0
29	2777.0
30	3295.0
31	3915.5
32	4618.0
33	5376.0
34	6089.5
35	6789.0
36	7605.5
37	8343.5
38	8997.5
39	9766.5
40	10747.5
41	11868.5
42	13176.5
43	14847.5
44	16891.0
45	19482.0
46	22917.0
47	27264.5
48	32688.5
49	39243.5
50	47062.5
51	55941.0
52	65871.0
53	76576.5
54	87713.5
55	99414.0
56	111178.5
57	122860.0
58	134567.5
59	146128.5
60	157279.5
61	168395.0
62	178960.0
63	187894.0
64	195099.0
65	199467.0
66	201058.5
67	199368.0
68	193122.5
69	182492.0
70	168438.0
71	151731.5
72	132120.5
73	111529.5
74	91609.0
75	72629.0
76	55150.5
77	40457.0
78	28626.0
79	19196.5
80	12322.5
81	7473.0
82	4291.0
83	2326.5
84	1201.5
85	668.5
86	402.5
87	247.5
88	170.0
89	122.5
90	95.0
91	76.0
92	64.0
93	49.5
94	35.5
95	30.5
96	19.5
97	11.5
98	9.5
99	7.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14265
2	0.10867500000000001
3	0.0815
4	0.10462500000000001
5	0.11472500000000001
6	0.0
7	0.002675
8	0.0085
9	0.017875000000000002
10-14	0.00294
15-19	1.35E-4
20-24	0.0
25-29	0.0
30-34	0.003835
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0011
55-59	9.999999999999999E-6
60-64	0.0
65-69	2.35E-4
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1994202319072443
#Duplication Level	Relative count
1	100.0
2	0.15825241259809397
3	0.0010015975480892022
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007987740446011E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	306895	3.2480447	4.106796	1
TTTTT	293565	3.2403219	3.7531908	9
>>END_MODULE