##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5384515	34.0	31.0	34.0	31.0	34.0
2	32.71347	34.0	31.0	34.0	31.0	34.0
3	32.73522925	34.0	31.0	34.0	31.0	34.0
4	36.12223075	37.0	37.0	37.0	35.0	37.0
5	36.12436925	37.0	37.0	37.0	35.0	37.0
6	36.1346175	37.0	37.0	37.0	35.0	37.0
7	36.14055425	37.0	37.0	37.0	35.0	37.0
8	36.13139375	37.0	37.0	37.0	35.0	37.0
9	37.86965475	39.0	39.0	39.0	35.0	39.0
10-14	38.1470369	39.4	38.4	39.4	35.4	39.4
15-19	39.218628349999996	41.0	39.4	41.0	36.0	41.0
20-24	38.97363700000001	41.0	39.0	41.0	35.6	41.0
25-29	38.640521250000006	40.2	38.2	41.0	34.8	41.0
30-34	37.949242149999996	40.0	37.2	41.0	33.4	41.0
35-39	36.949492299999996	38.6	35.0	40.8	32.6	41.0
40-44	35.94111635	36.6	35.0	40.0	31.2	41.0
45-49	35.010152999999995	35.0	34.8	38.4	30.6	41.0
50-54	33.70986555	34.8	33.4	36.0	28.6	39.2
55-59	33.8473154	35.0	34.8	35.0	30.4	38.0
60-64	33.4299912	35.0	34.0	35.0	30.0	36.2
65-69	33.0991417	35.0	34.0	35.0	29.2	35.0
70-74	32.825825699999996	35.0	33.4	35.0	29.0	35.0
75-79	32.50941425	35.0	33.0	35.0	27.8	35.0
80-84	32.202600600000004	35.0	33.0	35.0	27.0	35.0
85-89	31.9077548	35.0	33.0	35.0	26.2	35.0
90-94	31.443630050000003	35.0	33.0	35.0	24.2	35.0
95-99	30.851652950000005	35.0	32.0	35.0	22.0	35.0
100-101	29.598003	34.0	30.5	35.0	9.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6686.0
3	1313.0
4	1855.0
5	2322.0
6	3008.0
7	3708.0
8	4428.0
9	4942.0
10	5214.0
11	5442.0
12	5538.0
13	5623.0
14	5594.0
15	5902.0
16	6342.0
17	7041.0
18	7823.0
19	8901.0
20	10246.0
21	12196.0
22	14505.0
23	17581.0
24	21179.0
25	25359.0
26	30924.0
27	37886.0
28	46511.0
29	57215.0
30	71261.0
31	91003.0
32	119492.0
33	169553.0
34	280460.0
35	667313.0
36	1375567.0
37	721770.0
38	122086.0
39	16140.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.27114961266703	22.719675953588688	13.572550054152208	26.43662437959207
2	31.431841461368354	24.055452457694397	19.143344427831916	25.369361653105333
3	28.6826437565007	19.641985256154413	20.930907441079956	30.74446354626494
4	31.004539051494756	19.364307403349507	19.42651432149006	30.204639223665673
5	31.44291219175248	20.606469522497168	19.824464650745064	28.126153635005284
6	29.691094902486892	19.10857735683042	17.565489986576736	33.63483775410595
7	32.85047657019536	18.741042266245984	19.032792371675228	29.375688791883427
8	28.56442785316703	19.541974344779764	19.831576798683393	32.062021003369814
9	30.713322010046827	17.863927392177725	19.16284413999906	32.25990645777638
10-14	30.86450495582811	19.2361259968946	19.13216918643318	30.767199860844112
15-19	31.125283932464193	18.833047886079267	18.920604824467816	31.121063356988728
20-24	30.966150685914457	18.889615888403547	18.988018342389438	31.156215083292558
25-29	31.113439854859198	18.99395870003297	18.89918556304214	30.993415882065694
30-34	31.26188465006579	18.764186437533166	18.716415282968573	31.25751362943247
35-39	31.106882137954774	18.82570974779035	18.85448607725677	31.21292203699811
40-44	31.24741560510016	18.885570418892225	18.779999605996966	31.087014370010653
45-49	31.34160863594709	18.68541169910507	18.634196064031194	31.338783600916653
50-54	31.16447822252908	18.767469368415643	18.78800009520337	31.280052313851908
55-59	31.261370538205185	18.848384140425186	18.753892264761454	31.136353056608172
60-64	31.371769999999998	18.65514	18.620965	31.352124999999997
65-69	31.16404	18.76465	18.79151	31.2798
70-74	31.276335	18.849805	18.747015	31.126844999999996
75-79	31.385705	18.68047	18.60024	31.333585000000003
80-84	31.20791744950094	18.748042497650996	18.77833753400504	31.265702518843025
85-89	31.317394999999998	18.828345	18.723480000000002	31.13078
90-94	31.438905000000002	18.64607	18.59282	31.322204999999997
95-99	31.244905	18.71499	18.75594	31.284165
100-101	31.459631028764168	18.967792048894495	18.678714121980487	30.893862800360854
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.7077739922591
2	56.80120311447369
3	59.427107302765634
4	61.20917827516044
5	59.56906582675777
6	63.32593265659284
7	62.22616536207879
8	60.62644885653684
9	62.97322846782322
10-14	61.63170481667222
15-19	62.24634728945292
20-24	62.12236576920702
25-29	62.10685573692489
30-34	62.51939827949826
35-39	62.319804174952885
40-44	62.33442997511081
45-49	62.68039223686374
50-54	62.444530536380995
55-59	62.39772359481336
60-64	62.723895
65-69	62.443839999999994
70-74	62.403180000000006
75-79	62.71929000000001
80-84	62.47361996834396
85-89	62.44817499999999
90-94	62.761109999999995
95-99	62.52907
100-101	62.353493829125014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	122.0
1	81.0
2	38.5
3	36.0
4	31.5
5	33.0
6	38.5
7	37.5
8	38.0
9	43.5
10	47.0
11	52.0
12	63.0
13	76.0
14	96.0
15	119.5
16	131.5
17	153.5
18	191.5
19	246.5
20	330.0
21	450.0
22	556.5
23	689.0
24	895.0
25	1150.5
26	1462.5
27	1848.5
28	2296.5
29	2763.5
30	3333.0
31	3959.5
32	4611.5
33	5349.5
34	6085.5
35	6883.5
36	7696.0
37	8376.0
38	9102.5
39	9984.5
40	10901.0
41	11875.0
42	13110.5
43	14854.0
44	17035.5
45	19543.0
46	22942.5
47	27319.5
48	32699.5
49	39405.0
50	47321.0
51	56285.5
52	66101.5
53	76853.5
54	88222.5
55	99583.0
56	111454.0
57	123215.0
58	134676.5
59	146279.0
60	157321.0
61	167952.0
62	178392.0
63	187832.0
64	195022.5
65	199529.5
66	200616.5
67	198203.5
68	192232.0
69	181551.5
70	167309.5
71	150460.0
72	131267.0
73	111453.5
74	91494.0
75	72290.5
76	55475.0
77	41004.0
78	28976.5
79	19706.0
80	12763.0
81	7848.0
82	4631.0
83	2704.0
84	1568.5
85	907.0
86	590.5
87	418.5
88	307.5
89	231.5
90	177.0
91	148.5
92	124.0
93	94.5
94	83.5
95	64.0
96	45.0
97	38.0
98	26.5
99	15.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21182499999999999
2	0.17575
3	0.15495
4	0.171875
5	0.17965
6	0.042925
7	0.051449999999999996
8	0.061275
9	0.0879
10-14	0.025789999999999997
15-19	0.013635000000000001
20-24	0.007575
25-29	0.00331
30-34	0.023350000000000003
35-39	0.00462
40-44	7.700000000000001E-4
45-49	0.00124
50-54	0.00354
55-59	0.0019850000000000002
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	1.1999999999999999E-4
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0010125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.20193538067818154
#Duplication Level	Relative count
1	100.0
2	0.16778523489932887
3	0.0010017028949213663
4	5.008514474606832E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010017028949213663
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	312585	3.3116262	4.09261	1
TTTTT	297760	3.2956293	3.719242	10-14
>>END_MODULE