##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002007_ItsyBitsy_ATTCCT_L002_R2_005.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 62 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.622825 34.0 31.0 34.0 31.0 34.0 2 32.802252 34.0 31.0 34.0 31.0 34.0 3 32.83018825 34.0 31.0 34.0 31.0 34.0 4 36.2135655 37.0 37.0 37.0 35.0 37.0 5 36.21825275 37.0 37.0 37.0 35.0 37.0 6 36.1918525 37.0 37.0 37.0 35.0 37.0 7 36.1896065 37.0 37.0 37.0 35.0 37.0 8 36.1736665 37.0 37.0 37.0 35.0 37.0 9 37.9515125 39.0 39.0 39.0 37.0 39.0 10-14 38.243697749999995 39.4 39.2 39.4 36.6 39.4 15-19 39.411963400000005 41.0 40.0 41.0 37.0 41.0 20-24 39.22147285 41.0 39.2 41.0 36.0 41.0 25-29 38.83606350000001 40.4 38.6 41.0 35.0 41.0 30-34 38.16711925 40.0 37.4 41.0 34.2 41.0 35-39 37.203441350000006 39.0 35.2 40.8 33.0 41.0 40-44 36.20673605 37.0 35.0 40.0 32.0 41.0 45-49 35.290242299999996 35.0 35.0 38.8 31.4 41.0 50-54 34.01086865 34.8 33.8 36.2 29.8 39.2 55-59 34.1483828 35.0 35.0 35.0 31.0 38.2 60-64 33.75160805 35.0 35.0 35.0 31.0 36.4 65-69 33.4363994 35.0 34.2 35.0 30.4 35.0 70-74 33.1931201 35.0 34.0 35.0 30.0 35.0 75-79 32.97816025 35.0 34.0 35.0 29.2 35.0 80-84 32.731485799999994 35.0 33.0 35.0 29.0 35.0 85-89 32.4362621 35.0 33.0 35.0 27.8 35.0 90-94 32.1029472 35.0 33.0 35.0 27.0 35.0 95-99 31.623233199999998 35.0 33.0 35.0 25.6 35.0 100-101 30.601417 34.0 31.5 35.0 22.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6099.0 3 929.0 4 1205.0 5 1451.0 6 2006.0 7 2495.0 8 3083.0 9 3625.0 10 3832.0 11 4097.0 12 4069.0 13 4152.0 14 4263.0 15 4426.0 16 5002.0 17 5424.0 18 5994.0 19 7056.0 20 8008.0 21 9712.0 22 11924.0 23 14510.0 24 17361.0 25 21659.0 26 26845.0 27 32951.0 28 40315.0 29 49896.0 30 62864.0 31 80582.0 32 106976.0 33 151182.0 34 249153.0 35 635354.0 36 1467014.0 37 794299.0 38 132116.0 39 18014.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.262454711845905 22.668063807785433 13.557727216959991 26.511754263408672 2 31.443420369292763 24.08349445310007 19.109742896651838 25.36334228095533 3 28.73775298075667 19.611674444657464 20.878761955438446 30.771810619147423 4 31.037608610068922 19.35213250772537 19.362168544857205 30.2480903373485 5 31.410511034771886 20.5826810084286 19.82321838648921 28.183589570310303 6 29.69560214026419 19.020201779880363 17.590086564745747 33.6941095151097 7 32.89894831959544 18.679699302352606 19.031102611469308 29.39024976658265 8 28.60544910304124 19.460985681571188 19.82316050380872 32.11040471157885 9 30.757802863483004 17.78008202127505 19.162644813023245 32.2994703022187 10-14 30.882231615135137 19.205506548472957 19.096497161700945 30.81576467469096 15-19 31.091633084110764 18.776460588449257 18.928420361048094 31.203485966391888 20-24 30.991706096878346 18.8388905866411 18.97057478551786 31.198828530962697 25-29 31.151125288664332 18.941764170702452 18.887354949815574 31.019755590817645 30-34 31.255225859488554 18.723343893375624 18.709609314807203 31.311820932328622 35-39 31.093663418079228 18.808476650571343 18.858049608585294 31.23981032276414 40-44 31.267027931722957 18.86264783259724 18.749221242625406 31.121102993054393 45-49 31.376723532086153 18.63364207363342 18.617478332282097 31.37215606199833 50-54 31.16231769223227 18.74325443751463 18.79793926442605 31.296488605827044 55-59 31.283442404732813 18.82322758788236 18.728987377254967 31.16434263012986 60-64 31.38450626076914 18.61916626512217 18.58920105386743 31.407126420241262 65-69 31.1759146763872 18.72702280905342 18.77748781662317 31.319574697936204 70-74 31.300850000000004 18.81632 18.72116 31.16167 75-79 31.38491784909428 18.620575263113075 18.590531914781902 31.40397497301074 80-84 31.195609321384975 18.733751468909972 18.755056508617113 31.31558270108794 85-89 31.333392634984886 18.799903577314627 18.70519593970692 31.16150784799357 90-94 31.421378939583093 18.622371427919994 18.572036005102444 31.38421362739447 95-99 31.22066228946246 18.738689290095103 18.739019338166603 31.301629082275834 100-101 31.396611431595574 18.917129389942914 18.692186678945667 30.994072499515845 >>END_MODULE >>Per base GC content warn #Base %GC 1 63.774208975254574 2 56.80676265024809 3 59.50956359990409 4 61.28569894741742 5 59.59410060508219 6 63.389711655373894 7 62.28919808617809 8 60.71585381462009 9 63.05727316570171 10-14 61.697996289826094 15-19 62.295119050502656 20-24 62.190534627841046 25-29 62.17088087948197 30-34 62.56704679181717 35-39 62.33347374084336 40-44 62.38813092477735 45-49 62.748879594084485 50-54 62.45880629805932 55-59 62.447785034862676 60-64 62.7916326810104 65-69 62.49548937432341 70-74 62.46252 75-79 62.788892822105026 80-84 62.51119202247292 85-89 62.49490048297845 90-94 62.805592566977566 95-99 62.52229137173829 100-101 62.390683931111425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 4.5 2 3.0 3 2.5 4 4.0 5 6.0 6 10.0 7 11.5 8 17.0 9 28.5 10 43.0 11 58.5 12 82.5 13 109.0 14 123.5 15 156.0 16 209.0 17 256.5 18 287.5 19 332.5 20 421.5 21 515.0 22 585.5 23 744.5 24 946.0 25 1154.0 26 1475.5 27 1831.5 28 2229.0 29 2736.0 30 3264.0 31 3876.5 32 4591.5 33 5258.0 34 5945.0 35 6671.0 36 7431.0 37 8229.0 38 8974.5 39 9831.0 40 10760.5 41 11834.5 42 13215.0 43 14777.5 44 16716.5 45 19357.0 46 22955.5 47 27474.5 48 32742.0 49 39289.0 50 46892.5 51 55405.5 52 65415.5 53 76498.5 54 88004.5 55 99361.0 56 110768.0 57 122410.5 58 133990.5 59 145416.0 60 156825.0 61 168483.5 62 178741.5 63 186996.0 64 194654.0 65 199814.5 66 201171.0 67 198982.5 68 192874.0 69 182989.5 70 169005.5 71 151813.5 72 132643.5 73 112323.5 74 92264.0 75 73243.0 76 55894.0 77 40995.0 78 28776.0 79 19480.5 80 12611.5 81 7570.0 82 4314.5 83 2381.0 84 1282.5 85 711.0 86 418.0 87 265.5 88 171.0 89 128.5 90 104.5 91 81.5 92 61.0 93 44.0 94 39.0 95 31.0 96 25.5 97 18.5 98 13.0 99 10.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15325 2 0.113125 3 0.11345000000000001 4 0.109975 5 0.116875 6 0.175875 7 0.17880000000000001 8 0.18632500000000002 9 0.16005 10-14 0.12328 15-19 0.09198 20-24 0.06394 25-29 0.072085 30-34 0.106145 35-39 0.07657 40-44 0.041075 45-49 0.05408 50-54 0.045399999999999996 55-59 0.058585 60-64 7.05E-4 65-69 1.4999999999999999E-5 70-74 0.0 75-79 0.011145 80-84 0.023655 85-89 0.002785 90-94 8.399999999999999E-4 95-99 0.014564999999999998 100-101 0.0189875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.28966832976242296 #Duplication Level Relative count 1 100.0 2 0.1592795355849516 3 0.0035061532990398147 4 0.002504395213599868 5 0.0020035161708798943 6 0.0010017580854399472 7 5.008790427199736E-4 8 0.0 9 0.0 10++ 0.0070123065980796295 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 279715 3.103679 3.677742 10-14 AAAAA 285710 3.0511904 3.8982494 1 >>END_MODULE