##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70446525	34.0	31.0	34.0	31.0	34.0
2	32.878476	34.0	33.0	34.0	31.0	34.0
3	32.9128765	34.0	33.0	34.0	31.0	34.0
4	36.27179975	37.0	37.0	37.0	35.0	37.0
5	36.270234	37.0	37.0	37.0	35.0	37.0
6	36.25592575	37.0	37.0	37.0	35.0	37.0
7	36.24327125	37.0	37.0	37.0	35.0	37.0
8	36.22444975	37.0	37.0	37.0	35.0	37.0
9	38.00773275	39.0	39.0	39.0	37.0	39.0
10-14	38.30854655	39.4	39.2	39.4	37.0	39.4
15-19	39.4925615	41.0	40.0	41.0	37.0	41.0
20-24	39.289558449999994	41.0	39.6	41.0	36.4	41.0
25-29	38.90105930000001	40.6	38.8	41.0	35.2	41.0
30-34	38.2194511	40.0	37.4	41.0	34.4	41.0
35-39	37.224080300000004	39.0	35.2	40.8	33.0	41.0
40-44	36.21939385	37.0	35.0	40.0	32.2	41.0
45-49	35.290796	35.0	35.0	38.8	31.4	41.0
50-54	34.00230385	34.8	33.8	36.2	29.8	39.2
55-59	34.1268848	35.0	35.0	35.0	31.0	38.2
60-64	33.71898245	35.0	35.0	35.0	31.0	36.4
65-69	33.37989745	35.0	34.2	35.0	30.6	35.2
70-74	33.100207350000005	35.0	34.0	35.0	29.8	35.0
75-79	32.855233299999995	35.0	33.6	35.0	29.0	35.0
80-84	32.542551149999994	35.0	33.0	35.0	28.2	35.0
85-89	32.22358795	35.0	33.0	35.0	27.0	35.0
90-94	31.8608903	35.0	33.0	35.0	26.6	35.0
95-99	31.289235299999994	35.0	33.0	35.0	24.0	35.0
100-101	30.116999749999998	34.0	31.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7301.0
3	1083.0
4	1509.0
5	1755.0
6	2283.0
7	2820.0
8	3370.0
9	3915.0
10	4052.0
11	4360.0
12	4428.0
13	4513.0
14	4646.0
15	4801.0
16	5357.0
17	6059.0
18	6560.0
19	7346.0
20	8517.0
21	10015.0
22	12138.0
23	14886.0
24	17737.0
25	21318.0
26	25956.0
27	31640.0
28	39613.0
29	48869.0
30	61639.0
31	79543.0
32	105736.0
33	152535.0
34	256621.0
35	654301.0
36	1452433.0
37	781128.0
38	131483.0
39	17671.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.26591159908246	22.752618599028857	13.556124859169994	26.42534494271869
2	31.37339083728531	24.082817306459884	19.113713831389386	25.43007802486542
3	28.722271501752157	19.63118715764601	20.910410315303036	30.736131025298803
4	31.002281462479033	19.318284792803457	19.396210979957477	30.283222764760044
5	31.43531709964322	20.587878225046012	19.7685674113498	28.20823726396096
6	29.715104253548795	19.012452917711755	17.574624080583536	33.6978187481559
7	32.92193842327759	18.669859029044886	19.01101395080395	29.39718859687357
8	28.59244370210789	19.48758732769365	19.817253912676566	32.1027150575219
9	30.77293093236942	17.804899802280243	19.152736259078022	32.269433006272315
10-14	30.880756847392554	19.206744143437433	19.108985589889475	30.80351341928054
15-19	31.08359531116539	18.78750613906907	18.93034852892362	31.198550020841925
20-24	30.970079105012736	18.83888242683781	18.9710768935151	31.219961574634354
25-29	31.137868278903134	18.96144513529426	18.872421399568434	31.028265186234172
30-34	31.270592781219957	18.704557325701916	18.714560365164008	31.310289527914115
35-39	31.11597313509595	18.791034275183797	18.841867154672986	31.25112543504727
40-44	31.247876168782597	18.864139042351745	18.759123218433313	31.12886157043235
45-49	31.38002401975215	18.635581214160247	18.60322999823284	31.38116476785476
50-54	31.1597435839577	18.744505418768455	18.77469543928056	31.321055557993283
55-59	31.264533508376353	18.826164640947066	18.733057114839603	31.176244735836978
60-64	31.374422730532647	18.616564367942594	18.60300876404044	31.406004137484324
65-69	31.19774136442649	18.71552976959387	18.775685717050045	31.311043148929596
70-74	31.31866813186681	18.80608188060819	18.710936871093686	31.164313116431313
75-79	31.399311090874804	18.63798894186681	18.595460353218332	31.367239614040056
80-84	31.22624283274239	18.712143647805448	18.75348775234671	31.308125767105448
85-89	31.32402585226207	18.81247609165802	18.68493493180653	31.178563124273374
90-94	31.41542108749602	18.607484755439	18.570653400045124	31.40644075701986
95-99	31.243981719432135	18.72207182703444	18.717890416226435	31.31605603730699
100-101	31.48170539950646	18.8666557046405	18.647569399941606	31.004069495911434
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.69125654180115
2	56.80346886215073
3	59.45840252705096
4	61.28550422723907
5	59.64355436360419
6	63.412923001704705
7	62.319127020151164
8	60.695158759629784
9	63.042363938641735
10-14	61.68427026667309
15-19	62.28214533200731
20-24	62.1900406796471
25-29	62.16613346513731
30-34	62.58088230913407
35-39	62.36709857014322
40-44	62.37673773921494
45-49	62.76118878760691
50-54	62.48079914195098
55-59	62.440778244213334
60-64	62.78042686801697
65-69	62.50878451335608
70-74	62.48298124829812
75-79	62.766550704914856
80-84	62.53436859984783
85-89	62.50258897653544
90-94	62.82186184451588
95-99	62.56003775673913
100-101	62.48577489541789
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	6.5
2	12.5
3	9.5
4	3.5
5	7.0
6	15.5
7	30.5
8	44.5
9	62.0
10	90.0
11	124.5
12	167.0
13	185.5
14	194.5
15	235.5
16	257.0
17	274.0
18	307.0
19	348.5
20	401.0
21	506.5
22	625.0
23	758.5
24	978.5
25	1215.5
26	1474.0
27	1808.0
28	2238.5
29	2719.0
30	3278.0
31	3893.5
32	4576.0
33	5320.5
34	6014.0
35	6674.5
36	7376.5
37	8248.0
38	9124.0
39	9875.5
40	10783.0
41	11879.5
42	13078.5
43	14650.0
44	16738.5
45	19437.0
46	22875.0
47	27294.0
48	32600.0
49	39049.0
50	46759.0
51	55880.0
52	66031.5
53	76476.5
54	87774.0
55	99547.0
56	111212.0
57	123260.5
58	135067.0
59	146072.0
60	156825.5
61	167567.0
62	178260.5
63	187327.0
64	194132.0
65	198610.0
66	200214.5
67	198364.0
68	192560.0
69	182562.5
70	168445.0
71	151208.0
72	131865.5
73	111726.5
74	91774.0
75	72798.0
76	55843.0
77	41471.0
78	29489.5
79	19888.0
80	12795.0
81	7990.5
82	4772.0
83	2713.5
84	1565.0
85	931.0
86	614.5
87	411.5
88	290.5
89	234.5
90	182.5
91	147.0
92	120.0
93	87.0
94	68.0
95	55.0
96	45.0
97	36.5
98	28.0
99	19.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18994999999999998
2	0.1344
3	0.14447500000000002
4	0.12985
5	0.126425
6	0.18805
7	0.1993
8	0.22495
9	0.2123
10-14	0.146845
15-19	0.11718500000000001
20-24	0.079025
25-29	0.09406
30-34	0.130355
35-39	0.09419
40-44	0.06268
45-49	0.06558
50-54	0.066315
55-59	0.077895
60-64	0.004455
65-69	0.001575
70-74	9.999999999999999E-6
75-79	0.020195
80-84	0.034115
85-89	0.008749999999999999
90-94	0.00368
95-99	0.03374
100-101	0.0336875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.25380457048072713
#Duplication Level	Relative count
1	100.0
2	0.16779700069122347
3	0.003506205984592729
4	0.0015026597076825982
5	0.0
6	0.0015026597076825982
7	0.0010017731384550654
8	5.008865692275327E-4
9	0.0
10++	0.0015026597076825982
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	283135	3.1533372	3.8291283	9
AAAAA	288870	3.0908372	3.8856373	1
>>END_MODULE