##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73710475	34.0	31.0	34.0	31.0	34.0
2	32.9025445	34.0	33.0	34.0	31.0	34.0
3	32.93992675	34.0	33.0	34.0	31.0	34.0
4	36.302138	37.0	37.0	37.0	35.0	37.0
5	36.30292725	37.0	37.0	37.0	35.0	37.0
6	36.283886	37.0	37.0	37.0	35.0	37.0
7	36.26625075	37.0	37.0	37.0	35.0	37.0
8	36.24131275	37.0	37.0	37.0	35.0	37.0
9	38.02647175	39.0	39.0	39.0	37.0	39.0
10-14	38.33957305	39.4	39.2	39.4	37.2	39.4
15-19	39.52596525	41.0	40.0	41.0	37.0	41.0
20-24	39.3315086	41.0	39.6	41.0	36.6	41.0
25-29	38.9427754	40.6	38.8	41.0	35.2	41.0
30-34	38.26094105	40.0	37.4	41.0	34.6	41.0
35-39	37.2882	39.0	35.2	41.0	33.0	41.0
40-44	36.286220750000005	37.0	35.0	40.0	32.6	41.0
45-49	35.34627065	35.0	35.0	38.8	31.4	41.0
50-54	34.04507315	34.8	33.8	36.2	29.8	39.2
55-59	34.165531050000006	35.0	35.0	35.0	31.0	38.2
60-64	33.789448799999995	35.0	35.0	35.0	31.0	36.4
65-69	33.462847249999996	35.0	34.6	35.0	31.0	35.2
70-74	33.19019915	35.0	34.0	35.0	30.0	35.0
75-79	32.928321600000004	35.0	33.8	35.0	29.2	35.0
80-84	32.64494520000001	35.0	33.0	35.0	29.0	35.0
85-89	32.32831555	35.0	33.0	35.0	27.4	35.0
90-94	31.978799700000003	35.0	33.0	35.0	27.0	35.0
95-99	31.43358895	35.0	33.0	35.0	24.4	35.0
100-101	30.360617625	34.0	31.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7023.0
3	994.0
4	1361.0
5	1614.0
6	2070.0
7	2644.0
8	3200.0
9	3598.0
10	3832.0
11	4036.0
12	4177.0
13	4276.0
14	4357.0
15	4640.0
16	5008.0
17	5418.0
18	6158.0
19	7082.0
20	8174.0
21	9726.0
22	11800.0
23	14127.0
24	17359.0
25	20972.0
26	25336.0
27	31175.0
28	38126.0
29	47593.0
30	59746.0
31	77714.0
32	103455.0
33	149658.0
34	251611.0
35	639264.0
36	1462546.0
37	806297.0
38	135133.0
39	18625.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.23078140835559	22.727156591775667	13.581126405928357	26.460935593940388
2	31.44827420480052	24.039328741025212	19.122960764627063	25.389436289547206
3	28.67576849569468	19.60876355222686	20.921964508975403	30.793503443103056
4	31.010721805425796	19.31962172231122	19.398306524857166	30.271349947405817
5	31.437767300610105	20.575656441877452	19.826545348171752	28.160030909340694
6	29.70347161775444	19.038230966401663	17.554211786812882	33.70408562903101
7	32.87267417176908	18.691487190020027	19.036217608728595	29.399621029482304
8	28.634979183084862	19.474885015203927	19.824944966416044	32.06519083529516
9	30.663647845393584	17.862509146677628	19.17890400248589	32.2949390054429
10-14	30.858617965747325	19.22234556358863	19.126449055912826	30.79258741475122
15-19	31.07649009960812	18.78766290201586	18.941200345238993	31.19464665313703
20-24	30.96503938772896	18.8516865873575	18.982463654412708	31.200810370500832
25-29	31.116434839268898	18.972501003412102	18.908292638007946	31.002771519311057
30-34	31.268526912345813	18.722702122193166	18.7178610699096	31.290909895551415
35-39	31.111743281459503	18.80198548193273	18.84018591813027	31.246085318477494
40-44	31.25678973839097	18.86157992087142	18.76921971587179	31.112410624865817
45-49	31.34939192175894	18.648819840861382	18.642657299737547	31.35913093764214
50-54	31.161032326701594	18.750246406270858	18.789311181151085	31.29941008587646
55-59	31.273189137099166	18.816022463511054	18.752758779937547	31.158029619452233
60-64	31.377728826637295	18.620411172246705	18.6127861676717	31.389073833444296
65-69	31.15598	18.744325	18.78546	31.314235
70-74	31.3056	18.816740000000003	18.715055	31.162605
75-79	31.39178368172777	18.638627624247174	18.594542106944683	31.375046587080373
80-84	31.185156588331665	18.75009376397083	18.76326572659326	31.301483921104246
85-89	31.307029832926077	18.823087998874396	18.692347665486547	31.177534502712984
90-94	31.426	18.63371	18.558854999999998	31.381435000000003
95-99	31.23541812072871	18.740571726692547	18.72636367764522	31.297646474933522
100-101	31.429238039294237	18.906678749968428	18.680104338510496	30.983978872226835
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.69171700229598
2	56.837710494347725
3	59.46927193879774
4	61.28207175283161
5	59.597798209950795
6	63.40755724678545
7	62.27229520125138
8	60.70017001838003
9	62.95858685083648
10-14	61.65120538049854
15-19	62.27113675274515
20-24	62.1658497582298
25-29	62.119206358579945
30-34	62.55943680789723
35-39	62.357828599937
40-44	62.369200363256795
45-49	62.70852285940107
50-54	62.460442412578054
55-59	62.4312187565514
60-64	62.7668026600816
65-69	62.470214999999996
70-74	62.468205
75-79	62.766830268808135
80-84	62.48664050943591
85-89	62.48456433563906
90-94	62.807435
95-99	62.533064595662225
100-101	62.413216911521076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	8.5
2	5.5
3	7.5
4	6.5
5	4.0
6	7.0
7	9.0
8	14.5
9	20.5
10	26.0
11	38.0
12	61.5
13	87.0
14	112.5
15	148.5
16	202.0
17	256.0
18	291.5
19	346.0
20	459.5
21	560.0
22	660.5
23	825.5
24	1019.0
25	1265.0
26	1528.0
27	1841.5
28	2236.0
29	2712.5
30	3325.0
31	3984.0
32	4644.5
33	5293.0
34	6043.0
35	6753.5
36	7596.0
37	8412.0
38	9029.5
39	9836.0
40	10771.5
41	11886.0
42	13155.0
43	14548.5
44	16610.0
45	19559.0
46	23032.5
47	27358.0
48	32779.5
49	39363.5
50	47374.0
51	56306.5
52	65974.5
53	76375.5
54	87614.5
55	99519.0
56	111095.0
57	122921.0
58	134906.5
59	146413.5
60	157733.5
61	167994.5
62	178148.5
63	187404.0
64	194412.5
65	199268.0
66	200809.5
67	198779.0
68	192299.0
69	181829.0
70	168226.0
71	150926.5
72	131528.0
73	111793.0
74	92015.5
75	72898.5
76	55601.5
77	40901.0
78	28878.5
79	19544.0
80	12640.0
81	7712.0
82	4525.5
83	2605.5
84	1406.5
85	818.0
86	522.5
87	358.5
88	257.0
89	191.5
90	153.5
91	117.5
92	98.0
93	96.0
94	73.0
95	47.0
96	47.0
97	36.0
98	25.5
99	15.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19495
2	0.126475
3	0.14095
4	0.107775
5	0.094925
6	0.1647
7	0.190125
8	0.231375
9	0.23700000000000002
10-14	0.13713499999999998
15-19	0.11556999999999999
20-24	0.0704
25-29	0.0909
30-34	0.12502
35-39	0.07967500000000001
40-44	0.065185
45-49	0.041235
50-54	0.06342
55-59	0.06905
60-64	5.9999999999999995E-5
65-69	0.0
70-74	0.0
75-79	0.012515
80-84	0.014899999999999998
85-89	2.55E-4
90-94	0.0
95-99	0.02146
100-101	0.027325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.27874077108289347
#Duplication Level	Relative count
1	100.0
2	0.1768289017572685
3	0.002504658665117118
4	0.0
5	0.0
6	5.009317330234236E-4
7	5.009317330234236E-4
8	0.0
9	0.0
10++	0.006011180796281082
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	287160	3.1762729	3.6644516	10-14
AAAAA	294160	3.1347036	4.1342387	1
>>END_MODULE