##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53307875	34.0	31.0	34.0	31.0	34.0
2	32.68275	34.0	31.0	34.0	31.0	34.0
3	32.74766625	34.0	31.0	34.0	31.0	34.0
4	36.13821125	37.0	37.0	37.0	35.0	37.0
5	36.14788875	37.0	37.0	37.0	35.0	37.0
6	36.1404485	37.0	37.0	37.0	35.0	37.0
7	36.14410275	37.0	37.0	37.0	35.0	37.0
8	36.113246	37.0	37.0	37.0	35.0	37.0
9	37.87190675	39.0	38.0	39.0	35.0	39.0
10-14	38.157021099999994	39.4	38.4	39.4	35.2	39.4
15-19	39.2938753	41.0	39.4	41.0	36.0	41.0
20-24	39.079667900000004	41.0	39.0	41.0	35.6	41.0
25-29	38.7039446	40.2	38.4	41.0	35.0	41.0
30-34	38.0327666	40.0	37.2	41.0	33.4	41.0
35-39	37.045491899999995	38.6	35.2	40.8	32.6	41.0
40-44	36.02911354999999	36.8	35.0	40.0	31.2	41.0
45-49	35.123343999999996	35.0	35.0	38.4	31.0	41.0
50-54	33.81274245	34.8	33.4	36.0	29.2	39.2
55-59	33.94580010000001	35.0	35.0	35.0	31.0	38.0
60-64	33.556802149999996	35.0	34.0	35.0	30.2	36.2
65-69	33.2286859	35.0	34.0	35.0	29.6	35.0
70-74	32.97190055	35.0	33.8	35.0	29.0	35.0
75-79	32.70562525	35.0	33.0	35.0	29.0	35.0
80-84	32.418699749999995	35.0	33.0	35.0	27.4	35.0
85-89	32.11517445	35.0	33.0	35.0	27.0	35.0
90-94	31.744774	35.0	33.0	35.0	25.2	35.0
95-99	31.172306049999996	35.0	33.0	35.0	23.8	35.0
100-101	30.037374	34.0	30.5	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4558.0
3	1112.0
4	1585.0
5	1914.0
6	2339.0
7	3037.0
8	3701.0
9	4140.0
10	4555.0
11	4802.0
12	4793.0
13	4964.0
14	5069.0
15	4989.0
16	5498.0
17	6304.0
18	7223.0
19	8025.0
20	9458.0
21	11343.0
22	13632.0
23	16382.0
24	20275.0
25	24513.0
26	30200.0
27	36839.0
28	45739.0
29	56545.0
30	70975.0
31	89933.0
32	119250.0
33	167703.0
34	270709.0
35	645582.0
36	1401484.0
37	749469.0
38	124605.0
39	16693.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.21859193116377	22.74178076480747	13.57466777140359	26.46495953262517
2	31.394502467226708	24.10738556067896	19.14755152090998	25.350560451184357
3	28.715892891465916	19.63053301992568	20.903342837006665	30.750231251601747
4	31.029976194432823	19.307611374616336	19.380414565243072	30.281997865707773
5	31.431768653628932	20.601166983570476	19.82788331365668	28.139181049143907
6	29.76952900272643	19.017584231721653	17.60724380299657	33.60564296255534
7	32.9222900248137	18.684621386610107	19.021816490714315	29.371272097861876
8	28.599928986673866	19.477168765246407	19.849725993154397	32.07317625492533
9	30.722249573716947	17.834867351921677	19.161748400938222	32.28113467342315
10-14	30.87655321765372	19.22515940880183	19.117607265669196	30.780680107875252
15-19	31.07418457582789	18.799179080609292	18.94645844101836	31.180177902544454
20-24	30.983088803223964	18.84976957234798	18.967155026763415	31.19998659766464
25-29	31.12537327659874	18.96539436928026	18.895243799361268	31.01398855475973
30-34	31.268787821980126	18.729121446682047	18.70451784034055	31.297572890997273
35-39	31.11073970448068	18.806726507476252	18.856776384819543	31.225757403223525
40-44	31.276068286865886	18.869827855604	18.747523036108003	31.106580821422114
45-49	31.369536303031776	18.64501545336522	18.63191387200435	31.353534371598652
50-54	31.169827504598395	18.75488238054286	18.803180472888233	31.272109641970513
55-59	31.29825616680756	18.818204749573024	18.732124289522396	31.151414794097022
60-64	31.358539371837207	18.65794317513805	18.599743009267577	31.38377444375716
65-69	31.17623	18.756555	18.78345	31.283765000000002
70-74	31.309540000000002	18.81956	18.714285	31.156615
75-79	31.384919951755897	18.646303911165724	18.60797723820645	31.360798898871934
80-84	31.207639425409013	18.736885275862168	18.766036746553862	31.289438552174957
85-89	31.318588158870202	18.82011504158096	18.71022448663366	31.15107231291518
90-94	31.43534	18.633625000000002	18.58256	31.348474999999997
95-99	31.239410332300494	18.73103047839184	18.737480847030408	31.292078342277264
100-101	31.439659257927655	18.910679967751964	18.675632843941436	30.974027930378945
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.683551463788945
2	56.74506291841106
3	59.46612414306765
4	61.31197406014059
5	59.57094970277284
6	63.37517196528177
7	62.293562122675574
8	60.6731052415992
9	63.003384247140104
10-14	61.65723332552897
15-19	62.25436247837235
20-24	62.18307540088861
25-29	62.13936183135848
30-34	62.5663607129774
35-39	62.336497107704204
40-44	62.382649108288
45-49	62.723070674630435
50-54	62.441937146568904
55-59	62.44967096090458
60-64	62.74231381559437
65-69	62.459995
70-74	62.466155
75-79	62.74571885062783
80-84	62.49707797758397
85-89	62.46966047178538
90-94	62.783815
95-99	62.53148867457775
100-101	62.4136871883066
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	19.5
2	6.5
3	6.5
4	7.5
5	8.0
6	9.5
7	11.0
8	15.0
9	19.5
10	21.0
11	26.5
12	37.0
13	52.5
14	72.0
15	92.0
16	114.5
17	147.0
18	187.5
19	238.5
20	294.0
21	381.0
22	515.0
23	670.0
24	836.0
25	1063.0
26	1389.0
27	1758.5
28	2178.5
29	2640.0
30	3155.0
31	3817.5
32	4518.5
33	5252.5
34	5972.0
35	6701.5
36	7517.5
37	8304.0
38	9005.0
39	9712.5
40	10637.0
41	11724.0
42	12959.5
43	14662.5
44	16802.0
45	19418.5
46	22919.0
47	27366.5
48	32735.0
49	39271.0
50	46975.0
51	55903.0
52	65912.0
53	76457.5
54	87480.5
55	99551.5
56	111488.0
57	123126.5
58	135404.0
59	147097.0
60	158136.0
61	169175.0
62	179127.5
63	187872.0
64	194991.5
65	199312.0
66	200934.0
67	199012.5
68	192527.5
69	182134.0
70	168605.5
71	151404.0
72	131622.5
73	111493.5
74	91385.0
75	72293.5
76	55452.0
77	40664.5
78	28624.0
79	19372.0
80	12491.0
81	7691.0
82	4499.0
83	2529.5
84	1384.5
85	799.0
86	478.5
87	315.0
88	228.5
89	178.5
90	142.5
91	101.0
92	73.5
93	71.0
94	57.0
95	42.5
96	35.5
97	26.5
98	19.5
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.157225
2	0.12065000000000001
3	0.1088
4	0.10740000000000001
5	0.11427500000000002
6	0.0525
7	0.065275
8	0.08917499999999999
9	0.0664
10-14	0.034535
15-19	0.026725000000000002
20-24	0.017425
25-29	0.022195
30-34	0.03498
35-39	0.019735
40-44	0.016205
45-49	0.01207
50-54	0.016755
55-59	0.01796
60-64	2.85E-4
65-69	0.0
70-74	0.0
75-79	0.004365
80-84	0.005045
85-89	5.05E-4
90-94	0.0
95-99	0.005715
100-101	0.009412499999999999
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.18843969929622253
#Duplication Level	Relative count
1	100.0
2	0.15424138777186297
3	0.0020031349061280903
4	5.007837265320226E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	296950	3.15454	3.9979787	1
TTTTT	282470	3.1226017	3.6506193	10-14
>>END_MODULE