##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56355025	34.0	31.0	34.0	31.0	34.0
2	32.71498575	34.0	31.0	34.0	31.0	34.0
3	32.7737095	34.0	31.0	34.0	31.0	34.0
4	36.1464375	37.0	37.0	37.0	35.0	37.0
5	36.1448555	37.0	37.0	37.0	35.0	37.0
6	36.1433465	37.0	37.0	37.0	35.0	37.0
7	36.1425805	37.0	37.0	37.0	35.0	37.0
8	36.12911125	37.0	37.0	37.0	35.0	37.0
9	37.912989	39.0	39.0	39.0	35.0	39.0
10-14	38.2072468	39.4	39.0	39.4	35.4	39.4
15-19	39.35992055	41.0	40.0	41.0	36.6	41.0
20-24	39.14598135	41.0	39.0	41.0	35.8	41.0
25-29	38.7698872	40.4	38.4	41.0	35.0	41.0
30-34	38.0791731	40.0	37.2	41.0	33.8	41.0
35-39	37.1058665	38.8	35.2	40.8	32.8	41.0
40-44	36.062192550000006	36.8	35.0	40.0	31.6	41.0
45-49	35.0955004	35.0	35.0	38.4	30.8	41.0
50-54	33.7904584	34.8	33.4	36.2	29.2	39.2
55-59	33.96845525	35.0	35.0	35.0	31.0	38.2
60-64	33.56755705	35.0	34.4	35.0	30.4	36.4
65-69	33.24200365	35.0	34.0	35.0	29.8	35.0
70-74	32.9713548	35.0	34.0	35.0	29.0	35.0
75-79	32.68812435	35.0	33.2	35.0	29.0	35.0
80-84	32.375684	35.0	33.0	35.0	27.0	35.0
85-89	32.072628800000004	35.0	33.0	35.0	27.0	35.0
90-94	31.686761100000002	35.0	33.0	35.0	25.0	35.0
95-99	31.092584699999996	35.0	32.8	35.0	23.2	35.0
100-101	29.996562625	34.0	31.0	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5079.0
3	1199.0
4	1594.0
5	1798.0
6	2488.0
7	3095.0
8	3793.0
9	4147.0
10	4561.0
11	4742.0
12	5032.0
13	5050.0
14	5112.0
15	5290.0
16	5710.0
17	6530.0
18	7167.0
19	8143.0
20	9475.0
21	11341.0
22	13623.0
23	16443.0
24	20123.0
25	24375.0
26	29739.0
27	36272.0
28	44470.0
29	55763.0
30	69806.0
31	88820.0
32	117385.0
33	166494.0
34	272094.0
35	639839.0
36	1391844.0
37	764875.0
38	128776.0
39	17836.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.232598623154715	22.693683882036968	13.608685680483163	26.465031814325158
2	31.41551902798628	24.059900024653103	19.153581815230005	25.370999132130613
3	28.690105418521526	19.645901182692455	20.911309213217557	30.752684185568462
4	31.005966698915934	19.349034100217896	19.412113065161723	30.232886135704444
5	31.414262002442765	20.588894804422033	19.85656571983339	28.140277473301804
6	29.673686457784022	19.05187143678421	17.611505706526295	33.66293639890547
7	32.881458269604046	18.70184005955799	19.069947095721847	29.346754575116112
8	28.611054847609086	19.501750455609184	19.798663179191927	32.08853151758981
9	30.73304370590953	17.875883686507596	19.18906824164718	32.202004365935686
10-14	30.86387231013689	19.22758476177782	19.131344443473786	30.777198484611507
15-19	31.04379391238937	18.818090496300726	18.951278926064866	31.186836665245043
20-24	30.952233781428873	18.860345439944485	18.999737049802807	31.187683728823835
25-29	31.09256090525529	18.98240794898897	18.89982768815677	31.025203457598966
30-34	31.26342294267922	18.744616065570295	18.715306451796863	31.27665453995362
35-39	31.096273940336538	18.827184495150984	18.861845864177543	31.21469570033494
40-44	31.237905420166932	18.874152663719087	18.797800452252716	31.090141463861258
45-49	31.349989022024104	18.643948215155127	18.63804295916228	31.368019803658488
50-54	31.153966547491063	18.76360727054529	18.807785583918793	31.274640598044854
55-59	31.262160011588904	18.833709971063243	18.754833814779243	31.14929620256861
60-64	31.344050000000003	18.649684999999998	18.639734999999998	31.366529999999997
65-69	31.164835000000004	18.775415000000002	18.770755	31.288994999999996
70-74	31.28823	18.839235	18.72846	31.144074999999997
75-79	31.37297705946559	18.656902985354478	18.600792901189354	31.36932705399058
80-84	31.190825044662397	18.741375716287124	18.77768164753426	31.29011759151622
85-89	31.313845	18.825185	18.724855	31.136114999999997
90-94	31.430165	18.642055	18.57696	31.350820000000002
95-99	31.23278427342721	18.719490444993625	18.755050654798865	31.292674626780297
100-101	31.448243637822276	18.90200129425437	18.671313015681427	30.978442052241927
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.697630437479866
2	56.78651816011689
3	59.44278960408999
4	61.23885283462038
5	59.55453947574457
6	63.336622856689495
7	62.22821284472017
8	60.6995863651989
9	62.93504807184522
10-14	61.6410707947484
15-19	62.23063057763441
20-24	62.13991751025271
25-29	62.11776436285425
30-34	62.54007748263284
35-39	62.31096964067147
40-44	62.3280468840282
45-49	62.71800882568259
50-54	62.42860714553592
55-59	62.41145621415751
60-64	62.71058000000001
65-69	62.453829999999996
70-74	62.432305
75-79	62.74230411345617
80-84	62.48094263617862
85-89	62.449960000000004
90-94	62.780985
95-99	62.52545890020751
100-101	62.4266856900642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	7.0
2	6.0
3	5.0
4	4.0
5	7.5
6	6.5
7	6.0
8	8.5
9	11.0
10	13.5
11	23.5
12	33.5
13	44.0
14	52.0
15	64.5
16	101.0
17	135.0
18	178.0
19	226.0
20	285.0
21	383.5
22	501.5
23	662.0
24	882.5
25	1149.0
26	1461.5
27	1824.0
28	2218.0
29	2748.0
30	3325.5
31	3888.5
32	4613.5
33	5395.0
34	6090.5
35	6820.0
36	7598.5
37	8377.0
38	9127.0
39	9948.5
40	10883.0
41	11919.5
42	13199.0
43	14960.5
44	17150.0
45	19791.0
46	23327.5
47	27713.0
48	33018.0
49	39667.5
50	47360.5
51	56091.0
52	66047.5
53	77269.5
54	88490.0
55	99436.5
56	111298.0
57	123293.0
58	135073.0
59	146270.0
60	157202.0
61	168191.0
62	178346.0
63	187097.5
64	194476.5
65	199755.0
66	201039.0
67	198442.5
68	192086.5
69	181795.0
70	167886.0
71	150771.0
72	131240.0
73	110899.0
74	91077.0
75	72234.5
76	55332.0
77	40725.5
78	28720.0
79	19417.0
80	12591.5
81	7830.0
82	4701.5
83	2737.5
84	1540.5
85	927.5
86	634.5
87	441.0
88	318.0
89	252.5
90	181.5
91	141.5
92	113.0
93	90.5
94	73.5
95	56.0
96	45.0
97	35.0
98	26.0
99	19.0
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.261125
2	0.2154
3	0.200175
4	0.20445
5	0.215625
6	0.112175
7	0.13094999999999998
8	0.147425
9	0.11305
10-14	0.06787
15-19	0.04387
20-24	0.01909
25-29	0.01848
30-34	0.049859999999999995
35-39	0.018375
40-44	0.009445
45-49	0.004335
50-54	0.0075
55-59	0.007805
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.5000000000000001E-4
80-84	0.002565
85-89	0.0
90-94	0.0
95-99	5.9E-4
100-101	3.3749999999999996E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.20992232873836766
#Duplication Level	Relative count
1	100.0
2	0.16678854418143388
3	0.004507798491390105
4	0.0
5	5.008664990433449E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.001502599497130035
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	286120	3.1590931	3.8420105	10-14
AAAAA	293600	3.1203454	3.7972221	1
>>END_MODULE