##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_011.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2592787	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.569958889796965	34.0	31.0	34.0	31.0	34.0
2	32.829485414729405	34.0	31.0	34.0	31.0	34.0
3	32.87571829078131	34.0	31.0	34.0	31.0	34.0
4	36.33179817701956	37.0	37.0	37.0	35.0	37.0
5	36.23540460516039	37.0	37.0	37.0	35.0	37.0
6	36.224778973359555	37.0	37.0	37.0	35.0	37.0
7	36.20767151331752	37.0	37.0	37.0	35.0	37.0
8	36.19668796549813	37.0	37.0	37.0	35.0	37.0
9	37.955630369945546	39.0	38.0	39.0	35.0	39.0
10-14	38.20955959745247	39.4	38.2	39.4	35.2	39.4
15-19	39.31106249761358	41.0	39.0	41.0	36.0	41.0
20-24	39.084919586529864	40.2	38.8	41.0	35.4	41.0
25-29	38.55840630179031	40.0	38.0	41.0	34.4	41.0
30-34	37.55179048645338	39.6	36.6	41.0	32.6	41.0
35-39	36.828866621130075	38.4	35.0	40.4	31.6	41.0
40-44	35.90625330966254	36.6	35.0	40.0	31.0	41.0
45-49	35.082833800076905	35.0	34.8	38.4	30.2	40.8
50-54	33.92378255521954	35.0	33.6	36.2	28.4	39.6
55-59	33.536877113314745	35.0	33.0	35.0	29.2	38.0
60-64	33.27236259669614	35.0	33.0	35.0	29.0	36.2
65-69	32.92601590489308	35.0	33.0	35.0	29.0	35.0
70-74	32.61189276249842	35.0	33.0	35.0	27.6	35.0
75-79	32.091256397073884	34.4	32.6	35.0	26.6	35.0
80-84	32.290352119167515	35.0	33.0	35.0	27.0	35.0
85-89	31.9993708700329	35.0	33.0	35.0	27.0	35.0
90-94	31.56536414290877	35.0	33.0	35.0	24.8	35.0
95-99	30.999134599178415	35.0	32.4	35.0	23.6	35.0
100-101	29.704289245510722	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23.0
3	5.0
4	25.0
5	72.0
6	268.0
7	649.0
8	1483.0
9	2328.0
10	2958.0
11	3355.0
12	3459.0
13	3673.0
14	4000.0
15	4200.0
16	4477.0
17	4912.0
18	5360.0
19	5675.0
20	6486.0
21	7288.0
22	8892.0
23	10842.0
24	13680.0
25	17593.0
26	22123.0
27	27775.0
28	34948.0
29	43023.0
30	53890.0
31	67277.0
32	88465.0
33	122896.0
34	202942.0
35	477523.0
36	876407.0
37	395833.0
38	62135.0
39	5840.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.23905357670461	23.249023560296262	13.908412540085521	25.603510322913603
2	30.91603745313441	24.082926981661046	19.902097626993655	25.09893793821089
3	28.21180451768695	19.927282881316515	21.642541404288128	30.218371196708404
4	30.590634710834326	19.912318289161433	20.046613933192354	29.450433066811886
5	31.1940394640979	20.674239727366732	20.009858117924843	28.121862690610527
6	29.678411685958007	19.12332173834565	17.80620621747949	33.39206035821685
7	32.738593644599426	18.936920001527312	19.192089438893362	29.132396914979903
8	28.60906044345332	19.478653664955896	19.976612039477214	31.935673852113577
9	30.715288220744704	17.87420254729756	19.281144189630695	32.12936504232704
10-14	30.816839177302263	19.227364222359956	19.222496873055906	30.733299727281878
15-19	31.03982702782758	18.808610194358426	18.976946428688514	31.17461634912548
20-24	30.94164616245752	18.866587797203035	19.004100224697744	31.1876658156417
25-29	31.145037920626446	18.99862763887934	18.924382370000007	30.931952070494205
30-34	31.542221103086927	18.743974076544838	18.664858381439085	31.048946438929153
35-39	31.083094015533675	18.83910181886672	18.890359127547057	31.18744503805255
40-44	31.294584538070623	18.912855561450012	18.791942062744784	31.00061783773458
45-49	31.564706240813457	18.685576562980298	18.63546832038266	31.11424887582358
50-54	31.369158012334374	18.79615835659724	18.744029106603545	31.09065452446484
55-59	31.286333971899737	18.873204779258767	18.782028759014914	31.058432489826583
60-64	31.370935665242396	18.661981392412365	18.615085963895073	31.351996978450163
65-69	31.18064892877613	18.747208446056185	18.79043847935812	31.281704145809563
70-74	31.3026407491244	18.85282516458159	18.748450991153533	31.096083095140482
75-79	31.374771626053356	18.65109629136524	18.63467380853113	31.33945827405028
80-84	31.19286443342993	18.73522821520431	18.788884891464793	31.283022459900966
85-89	31.275701150131045	18.841555104422937	18.75590967357543	31.12683407187058
90-94	31.423507420947754	18.656160053939672	18.59967460307052	31.320657922042056
95-99	31.252910478184287	18.714310122659516	18.767912674662437	31.26486672449376
100-101	31.466738301295095	18.877293044125874	18.70842070713869	30.94754794744034
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.84256389961822
2	56.0149753913453
3	58.43017571439536
4	60.04106777764622
5	59.31590215470843
6	63.07047204417486
7	61.87099055957933
8	60.54473429556689
9	62.84465326307175
10-14	61.55013890458414
15-19	62.214443376953064
20-24	62.129311978099224
25-29	62.076989991120655
30-34	62.59116754201608
35-39	62.270539053586226
40-44	62.295202375805204
45-49	62.678955116637034
50-54	62.45981253679922
55-59	62.34476646172632
60-64	62.722932643692566
65-69	62.4623530745857
70-74	62.39872384426488
75-79	62.71422990010363
80-84	62.4758868933309
85-89	62.40253522200163
90-94	62.74416534298981
95-99	62.51777720267805
100-101	62.41428624873543
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.5
6	1.5
7	2.5
8	1.5
9	1.5
10	6.0
11	11.0
12	15.0
13	22.5
14	33.5
15	43.5
16	52.5
17	71.0
18	92.0
19	123.0
20	172.0
21	235.0
22	299.5
23	390.0
24	544.0
25	695.5
26	879.0
27	1149.0
28	1439.5
29	1751.5
30	2120.5
31	2555.0
32	2986.0
33	3447.0
34	3936.0
35	4434.0
36	4926.0
37	5423.0
38	5977.5
39	6512.5
40	7021.0
41	7694.0
42	8571.5
43	9653.5
44	11090.5
45	12921.5
46	15170.5
47	17889.0
48	21501.5
49	25948.0
50	30937.5
51	36710.0
52	43266.0
53	50330.0
54	57634.0
55	64930.0
56	72907.0
57	80871.0
58	88127.5
59	95502.5
60	102658.0
61	109707.0
62	116565.5
63	122241.5
64	126700.0
65	129415.0
66	129979.5
67	128236.0
68	123903.5
69	117318.0
70	108532.5
71	97326.0
72	84520.0
73	71509.0
74	58646.5
75	46578.5
76	35462.0
77	25786.0
78	18058.5
79	12137.5
80	7770.5
81	4681.0
82	2694.0
83	1441.0
84	753.0
85	416.5
86	246.5
87	148.0
88	106.5
89	69.5
90	42.5
91	33.5
92	27.0
93	17.5
94	12.0
95	12.0
96	5.5
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007906549978845158
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0057312845212506855
25-29	0.0011262012652794079
30-34	0.07319536853586507
35-39	0.02955892635993624
40-44	0.020078780092618483
45-49	0.0
50-54	3.08548291857372E-5
55-59	0.0
60-64	0.00810710636855245
65-69	0.0055924377899148675
70-74	0.0
75-79	0.0
80-84	2.3912492618946332E-4
85-89	1.619878532251203E-4
90-94	0.010066388021846761
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2592787.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.10848210045342739
#Duplication Level	Relative count
1	100.0
2	0.09108379709332586
3	0.0
4	0.0
5	0.0
6	0.0
7	5.004604235897025E-4
8	0.0
9	0.0
10++	5.004604235897025E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	186365	3.135006	3.65436	10-14
AAAAA	189015	3.0593803	3.8383293	1
>>END_MODULE