##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_010.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7527035	34.0	31.0	34.0	31.0	34.0
2	32.9987325	34.0	31.0	34.0	31.0	34.0
3	33.06458225	34.0	33.0	34.0	31.0	34.0
4	36.46118125	37.0	37.0	37.0	35.0	37.0
5	36.39657	37.0	37.0	37.0	35.0	37.0
6	36.374352	37.0	37.0	37.0	35.0	37.0
7	36.375482	37.0	37.0	37.0	35.0	37.0
8	36.3592785	37.0	37.0	37.0	35.0	37.0
9	38.16469675	39.0	39.0	39.0	37.0	39.0
10-14	38.42545705	39.4	39.0	39.4	37.0	39.4
15-19	39.59911559999999	41.0	40.0	41.0	37.0	41.0
20-24	39.36347225	41.0	39.2	41.0	36.4	41.0
25-29	38.8059546	40.0	38.4	41.0	35.2	41.0
30-34	37.74173935	40.0	37.0	41.0	33.0	41.0
35-39	37.087014	38.6	35.2	40.6	32.6	41.0
40-44	36.1891801	36.8	35.0	40.0	32.0	41.0
45-49	35.523364099999995	35.0	35.0	39.0	32.2	41.0
50-54	34.606872800000005	35.0	35.0	36.6	31.2	40.0
55-59	33.681269199999996	35.0	34.0	35.0	30.0	38.0
60-64	33.345859399999995	35.0	34.0	35.0	29.8	36.2
65-69	32.9395446	35.0	33.0	35.0	29.0	35.0
70-74	32.596315000000004	35.0	33.0	35.0	28.2	35.0
75-79	32.12662315	34.6	32.6	35.0	27.0	35.0
80-84	32.2940945	35.0	33.0	35.0	27.0	35.0
85-89	31.197976500000003	35.0	32.0	35.0	21.6	35.0
90-94	31.19368345	35.0	32.4	35.0	23.8	35.0
95-99	30.884256649999998	35.0	32.4	35.0	22.2	35.0
100-101	29.450965	34.0	30.5	35.0	9.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42.0
3	10.0
4	29.0
5	89.0
6	335.0
7	959.0
8	2107.0
9	3556.0
10	4511.0
11	5096.0
12	5309.0
13	5596.0
14	6195.0
15	6506.0
16	7425.0
17	8104.0
18	8906.0
19	9577.0
20	10337.0
21	11625.0
22	13737.0
23	16220.0
24	20135.0
25	24978.0
26	30755.0
27	38145.0
28	47538.0
29	59001.0
30	74379.0
31	94891.0
32	126395.0
33	181418.0
34	310921.0
35	742367.0
36	1355419.0
37	648954.0
38	107144.0
39	11269.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.437489140367084	23.03812215540119	13.781877319586341	25.742511384645383
2	31.075975	24.0345	19.764	25.125525
3	28.303099999999997	19.814899999999998	21.564425	30.317575
4	30.704825000000003	19.7757	19.94355	29.575924999999998
5	31.263049999999996	20.633399999999998	20.016475	28.087075
6	29.68925	19.09	17.82175	33.399
7	32.77545	18.86365	19.151375	29.209524999999996
8	28.566125	19.500925	19.951375	31.981575
9	30.678975	17.8664	19.26455	32.190075
10-14	30.839585000000003	19.22503	19.190335	30.745050000000003
15-19	31.07168	18.813255	18.94548	31.169584999999998
20-24	30.94344766423005	18.868698265500086	19.003418356034867	31.184435714234997
25-29	31.199076833583995	18.9835469356512	18.90544139281768	30.911934837947125
30-34	31.573555192316082	18.709783605622818	18.66345455950275	31.05320664255835
35-39	31.085956754833955	18.825923971943478	18.874435087336014	31.213684185886553
40-44	31.285186999555727	18.875384237784452	18.788400024564016	31.051028738095805
45-49	31.340459999999997	18.663050000000002	18.664415	31.332075
50-54	31.168225518693177	18.773039404951227	18.801429441858275	31.257305634497325
55-59	31.38614	18.854380000000003	18.74988	31.0096
60-64	31.388967773215658	18.639365565285864	18.61853443468413	31.353132226814353
65-69	31.241764167307462	18.73977076512489	18.775880786223325	31.24258428134432
70-74	31.367465	18.82995	18.72722	31.075364999999998
75-79	31.412785	18.61775	18.604545	31.36492
80-84	31.240350910965002	18.69343140693637	18.77545163249199	31.29076604960664
85-89	31.341123194112953	18.83205594218161	18.69683099982548	31.12998986387996
90-94	31.43561982059966	18.59514312546931	18.590952042912683	31.37828501101835
95-99	31.303609391082816	18.666840600052183	18.720010616003186	31.30953939286182
100-101	31.67675	18.836225000000002	18.60985	30.877175
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.18000052501247
2	56.2015
3	58.620675
4	60.280750000000005
5	59.350125
6	63.08825
7	61.984975
8	60.547700000000006
9	62.86905
10-14	61.584635
15-19	62.241265
20-24	62.12788337846504
25-29	62.11101167153112
30-34	62.626761834874436
35-39	62.2996409407205
40-44	62.336215737651536
45-49	62.672534999999996
50-54	62.42553115319049
55-59	62.395739999999996
60-64	62.74210000003001
65-69	62.48434844865179
70-74	62.44283000000001
75-79	62.777705
80-84	62.531116960571644
85-89	62.47111305799291
90-94	62.813904831618004
95-99	62.61314878394464
100-101	62.553925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.0
2	0.5
3	0.5
4	1.5
5	2.0
6	6.0
7	7.5
8	8.5
9	10.5
10	13.5
11	21.0
12	26.5
13	35.0
14	49.0
15	61.0
16	81.0
17	112.5
18	146.5
19	200.0
20	280.0
21	371.0
22	497.5
23	636.5
24	820.0
25	1089.0
26	1385.5
27	1755.5
28	2228.5
29	2695.5
30	3285.0
31	3875.5
32	4574.5
33	5404.0
34	6110.5
35	6825.0
36	7561.0
37	8391.0
38	9181.5
39	10041.5
40	10945.5
41	11970.0
42	13323.0
43	14863.0
44	16884.5
45	19702.5
46	23267.5
47	27692.5
48	33263.5
49	39949.0
50	47729.0
51	56718.5
52	66710.0
53	77181.5
54	88307.5
55	100295.0
56	112284.0
57	124042.0
58	135624.0
59	146563.0
60	157765.0
61	169253.0
62	179062.0
63	187262.0
64	194103.5
65	198212.0
66	199721.5
67	197626.5
68	191352.5
69	180749.0
70	166651.5
71	150188.0
72	130975.0
73	110799.0
74	90919.0
75	72231.5
76	55382.5
77	40904.0
78	29086.0
79	19694.5
80	12830.5
81	8003.0
82	4738.0
83	2774.0
84	1634.5
85	977.5
86	612.0
87	415.5
88	301.0
89	200.0
90	135.0
91	112.5
92	80.5
93	53.5
94	38.0
95	24.0
96	11.5
97	6.5
98	3.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002375
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00749
25-29	6.950000000000001E-4
30-34	0.08428
35-39	0.03322
40-44	0.01634
45-49	0.0
50-54	1.3000000000000002E-4
55-59	0.0
60-64	0.02943
65-69	0.013904999999999999
70-74	0.0
75-79	0.0
80-84	2.75E-4
85-89	0.01105
90-94	0.02583
95-99	2.9999999999999997E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.1444841067482601
#Duplication Level	Relative count
1	100.0
2	0.13117511878357607
3	0.0015020051769111765
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010013367846074509
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	296365	3.2513702	3.6278067	10-14
AAAAA	293650	3.118091	3.995275	1
>>END_MODULE