##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73781325	34.0	31.0	34.0	31.0	34.0
2	32.991667	34.0	31.0	34.0	31.0	34.0
3	33.0509895	34.0	31.0	34.0	31.0	34.0
4	36.454591	37.0	37.0	37.0	35.0	37.0
5	36.37832225	37.0	37.0	37.0	35.0	37.0
6	36.3618355	37.0	37.0	37.0	35.0	37.0
7	36.363095	37.0	37.0	37.0	35.0	37.0
8	36.34766175	37.0	37.0	37.0	35.0	37.0
9	38.14183675	39.0	39.0	39.0	37.0	39.0
10-14	38.398772550000004	39.4	38.6	39.4	37.0	39.4
15-19	39.53919005	41.0	40.0	41.0	37.0	41.0
20-24	39.10916830000001	40.8	38.8	41.0	35.6	41.0
25-29	36.7067158	39.4	35.0	41.0	27.0	41.0
30-34	34.594524	38.2	32.4	40.2	21.2	41.0
35-39	36.42510180000001	38.4	35.0	40.0	30.8	41.0
40-44	26.8221571	26.0	23.4	30.8	18.0	38.6
45-49	32.11678565	33.0	30.6	36.0	25.6	38.4
50-54	32.010124450000006	35.0	29.2	35.8	21.2	39.2
55-59	28.73788475	33.0	23.6	35.0	15.2	36.0
60-64	32.931815500000006	35.0	33.0	35.0	27.6	35.8
65-69	32.624716750000005	35.0	33.0	35.0	28.0	35.0
70-74	31.155501249999997	34.6	31.0	35.0	20.8	35.0
75-79	31.16383675	34.2	31.6	35.0	22.0	35.0
80-84	31.4375774	34.8	31.8	35.0	24.0	35.0
85-89	31.5518915	35.0	32.6	35.0	24.8	35.0
90-94	31.40625025	35.0	32.8	35.0	24.0	35.0
95-99	30.928673800000002	35.0	32.4	35.0	23.0	35.0
100-101	29.581130625	34.0	30.5	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33.0
3	10.0
4	32.0
5	122.0
6	374.0
7	1375.0
8	3804.0
9	5488.0
10	6958.0
11	8720.0
12	9974.0
13	8326.0
14	7221.0
15	7222.0
16	7158.0
17	7284.0
18	8147.0
19	9247.0
20	10375.0
21	11659.0
22	14975.0
23	19565.0
24	25875.0
25	33388.0
26	43451.0
27	55692.0
28	70149.0
29	90902.0
30	121725.0
31	176475.0
32	296443.0
33	567918.0
34	923438.0
35	844390.0
36	442481.0
37	136311.0
38	21271.0
39	2018.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.32313845218136	23.08000684238115	13.830713088154678	25.766141617282813
2	30.995275	24.038925	19.82745	25.13835
3	28.2646	19.8146	21.6205	30.300300000000004
4	30.6674	19.780675	19.9848	29.567125
5	31.157425	20.646875	20.07635	28.119349999999997
6	29.653525	19.125149999999998	17.794425	33.426899999999996
7	32.820875	18.858825000000003	19.166349999999998	29.153950000000002
8	28.504049999999996	19.549025	19.977925	31.968999999999998
9	30.7108	17.851475	19.286325	32.1514
10-14	30.82317	19.24034	19.192185000000002	30.744305
15-19	31.065435	18.792265	18.966459999999998	31.17584
20-24	31.279562747714067	18.827806786938613	18.919898532077184	30.972731933270136
25-29	31.471495729980802	18.83177990892206	18.74087396804557	30.95585039305157
30-34	31.941920627190186	18.771818047353094	18.521693453195446	30.764567872261278
35-39	31.28633871938481	18.90135751901404	18.789487410362014	31.022816351239136
40-44	38.320322016913096	17.27526862192792	16.834802197105912	27.56960716405307
45-49	31.45497	18.726509999999998	18.692515	31.126005
50-54	31.60557144757867	18.81762314723458	18.723252680100767	30.853552725085986
55-59	31.551606919343367	19.347253227632514	18.632025948765556	30.469113904258567
60-64	31.42900433398324	18.668371523424863	18.601964118999266	31.30066002359263
65-69	31.25430560119056	18.787494701519737	18.768033396639243	31.190166300650457
70-74	31.398225000000004	19.019385	18.65394	30.92845
75-79	31.555139999999998	18.86637	18.540005	31.038484999999998
80-84	31.23438090704277	18.774821610902144	18.75906658017983	31.23173090187526
85-89	31.414864316397285	18.795971501075716	18.709483089107863	31.079681093419136
90-94	31.440919197012285	18.593792872831706	18.586546918901874	31.378741011254128
95-99	31.287925	18.678485000000002	18.742315	31.291275000000002
100-101	31.54245	18.8289625	18.6691125	30.959474999999998
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.08928006946417
2	56.133624999999995
3	58.564899999999994
4	60.234525000000005
5	59.276775
6	63.080425
7	61.974825
8	60.47304999999999
9	62.8622
10-14	61.567475
15-19	62.241274999999995
20-24	62.2522946809842
25-29	62.427346123032365
30-34	62.70648849945146
35-39	62.309155070623945
40-44	65.88992918096616
45-49	62.580975
50-54	62.45912417266466
55-59	62.02072082360193
60-64	62.729664357575864
65-69	62.44447190184101
70-74	62.326674999999994
75-79	62.593624999999996
80-84	62.46611180891802
85-89	62.49454540981642
90-94	62.81966020826641
95-99	62.5792
100-101	62.501925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.5
5	2.0
6	4.0
7	5.5
8	5.0
9	7.0
10	9.0
11	14.5
12	18.0
13	23.5
14	37.5
15	55.5
16	71.5
17	96.0
18	123.0
19	155.5
20	221.5
21	292.0
22	390.5
23	524.0
24	693.0
25	910.0
26	1175.0
27	1514.5
28	1896.5
29	2321.5
30	2874.0
31	3503.5
32	4165.0
33	4881.0
34	5689.5
35	6495.5
36	7279.5
37	8099.5
38	8899.0
39	9734.0
40	10678.0
41	11748.5
42	13121.5
43	14708.5
44	16527.0
45	19219.5
46	22938.5
47	27324.0
48	32589.5
49	39424.5
50	47418.5
51	56384.5
52	66532.0
53	77551.0
54	89226.0
55	101443.5
56	113592.0
57	125648.0
58	137773.5
59	150247.0
60	162272.5
61	173045.5
62	183373.5
63	192367.0
64	199037.0
65	203299.0
66	204103.0
67	200838.0
68	193690.5
69	181393.0
70	165380.0
71	147392.0
72	127413.0
73	106788.5
74	86605.0
75	67795.0
76	51027.5
77	36946.5
78	25822.0
79	17305.5
80	11060.5
81	6744.0
82	4018.5
83	2327.5
84	1286.0
85	775.0
86	515.5
87	345.0
88	232.0
89	154.5
90	117.0
91	89.5
92	59.5
93	41.0
94	25.0
95	12.5
96	9.0
97	5.0
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0348
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.001895
25-29	0.001035
30-34	2.2727049999999998
35-39	0.017975
40-44	2.702005
45-49	0.0
50-54	4.95E-4
55-59	2.27512
60-64	0.01115
65-69	0.006705
70-74	0.0
75-79	0.0
80-84	1.95E-4
85-89	0.003945
90-94	0.013165
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.040497570145782084
#Duplication Level	Relative count
1	100.0
2	0.034011734048246645
3	0.0
4	5.001725595330389E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	268065	3.104815	3.8111324	95-97
>>END_MODULE