##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82703025	34.0	31.0	34.0	31.0	34.0
2	33.067615	34.0	31.0	34.0	31.0	34.0
3	33.04657875	34.0	33.0	34.0	31.0	34.0
4	36.473375	37.0	37.0	37.0	35.0	37.0
5	36.42568675	37.0	37.0	37.0	35.0	37.0
6	36.4228875	37.0	37.0	37.0	35.0	37.0
7	36.42479925	37.0	37.0	37.0	35.0	37.0
8	36.41190725	37.0	37.0	37.0	35.0	37.0
9	38.23912075	39.0	39.0	39.0	37.0	39.0
10-14	38.093971749999994	39.4	38.6	39.4	35.8	39.4
15-19	39.24842315	41.0	39.2	41.0	36.4	41.0
20-24	39.22454445	41.0	39.0	41.0	35.8	41.0
25-29	36.7304051	39.6	35.8	41.0	26.4	41.0
30-34	32.2577701	36.8	26.8	40.2	14.6	41.0
35-39	34.8594373	37.4	33.8	40.0	25.2	41.0
40-44	24.581318099999997	25.4	21.0	29.6	12.0	33.6
45-49	30.35938375	32.2	28.8	35.0	19.6	37.6
50-54	32.6119734	35.0	32.2	35.8	22.6	39.2
55-59	31.250338250000006	35.0	31.2	35.0	18.8	36.6
60-64	32.36072255	35.0	33.0	35.0	25.4	35.6
65-69	31.27569655	35.0	31.6	35.0	20.2	35.0
70-74	30.665516750000002	34.6	30.2	35.0	19.2	35.0
75-79	31.423418249999997	34.4	32.4	35.0	24.2	35.0
80-84	31.92278495	35.0	33.0	35.0	27.0	35.0
85-89	30.588140650000003	34.6	31.0	35.0	19.6	35.0
90-94	30.7800161	34.6	31.4	35.0	22.4	35.0
95-99	29.7371462	34.6	30.6	35.0	11.8	35.0
100-101	26.922043625	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40.0
3	18.0
4	40.0
5	157.0
6	420.0
7	1508.0
8	4416.0
9	8206.0
10	9697.0
11	10576.0
12	10494.0
13	9219.0
14	8295.0
15	8911.0
16	8905.0
17	8785.0
18	9515.0
19	10700.0
20	12245.0
21	14030.0
22	17669.0
23	23112.0
24	30042.0
25	39343.0
26	51207.0
27	66865.0
28	90314.0
29	121451.0
30	163030.0
31	234804.0
32	377936.0
33	620139.0
34	867553.0
35	735233.0
36	324129.0
37	88550.0
38	11593.0
39	850.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.35121626166284	23.054175121176094	13.773880472927441	25.82072814423362
2	31.036599999999996	23.9994	19.78885	25.175150000000002
3	28.715675	19.6477	21.4722	30.164425
4	30.738975000000003	19.776425	19.90525	29.579349999999998
5	31.282075	20.624775	20.020225	28.072924999999998
6	29.715825000000002	19.1019	17.79965	33.382625
7	32.756975	18.86635	19.17385	29.202824999999997
8	28.540425000000003	19.523375	19.961175	31.975025000000002
9	30.672424999999997	17.866475	19.274225	32.186875
10-14	31.03150155157508	19.270730963536547	19.094540954727048	30.603226530161326
15-19	31.127399999999998	18.75855	18.90895	31.2051
20-24	31.179399767395115	18.84929083510754	18.94722789178573	31.02408150571162
25-29	31.87895326156595	18.856480457595424	18.718849615166164	30.545716665672458
30-34	32.957402192675964	18.752168602396004	18.08218456535519	30.20824463957285
35-39	32.20734660559022	18.841569473872497	18.541491258911822	30.409592661625457
40-44	40.350783375942605	17.225580297980308	16.18559216745154	26.238044158625552
45-49	31.77966	18.78695	18.652935	30.780455
50-54	31.831362056657746	19.04806119085201	18.67174587098399	30.44883088150625
55-59	31.71313219919254	19.171448420013895	18.63279804295863	30.482621337834935
60-64	31.58830798790974	18.724066722097692	18.566486422466053	31.121138867526515
65-69	31.427785231314537	19.104968436580737	18.657931274956887	30.809315057147842
70-74	31.64360152776232	19.045157550864158	18.615330644658854	30.695910276714667
75-79	31.633860000000002	18.627645	18.550449999999998	31.188045
80-84	31.28266995175226	18.711277267701355	18.75286419525545	31.253188585290932
85-89	31.405197473586494	18.924588717567055	18.70457787407545	30.965635934770997
90-94	31.499883300881343	18.590504743446516	18.574768119901666	31.334843835770478
95-99	31.279054313321414	18.71524545353905	18.754757069564146	31.25094316357539
100-101	31.952125	19.13755	18.4702375	30.4400875
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.17194440589646
2	56.21175
3	58.8801
4	60.318324999999994
5	59.355000000000004
6	63.09844999999999
7	61.9598
8	60.51545
9	62.8593
10-14	61.6347280817364
15-19	62.3325
20-24	62.20348127310673
25-29	62.424669927238405
30-34	63.16564683224881
35-39	62.61693926721568
40-44	66.58882753456815
45-49	62.560115
50-54	62.28019293816401
55-59	62.19575353702748
60-64	62.709446855436255
65-69	62.23710028846238
70-74	62.339511804476984
75-79	62.821905
80-84	62.53585853704319
85-89	62.3708334083575
90-94	62.834727136651814
95-99	62.5299974768968
100-101	62.3922125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	4.0
7	4.0
8	3.0
9	4.5
10	5.0
11	5.0
12	10.5
13	16.5
14	24.0
15	41.0
16	65.5
17	89.5
18	109.5
19	142.5
20	196.0
21	256.0
22	342.0
23	455.0
24	596.5
25	782.5
26	1043.0
27	1360.0
28	1720.0
29	2130.0
30	2598.5
31	3235.5
32	4021.0
33	4756.5
34	5410.5
35	6211.5
36	7011.0
37	7766.5
38	8521.0
39	9440.5
40	10466.5
41	11483.0
42	12766.0
43	14421.5
44	16339.0
45	18872.0
46	22200.0
47	26221.5
48	31544.5
49	37826.0
50	45398.5
51	54609.0
52	64602.5
53	75610.0
54	87197.0
55	99452.0
56	111828.0
57	124300.0
58	136985.5
59	149005.0
60	161157.5
61	172608.0
62	182840.0
63	192290.5
64	199727.5
65	203638.0
66	204506.5
67	201363.0
68	193893.0
69	182439.5
70	167874.5
71	150274.0
72	129692.0
73	108838.0
74	88598.0
75	70050.5
76	53416.5
77	38949.5
78	27469.5
79	18651.5
80	12122.0
81	7633.0
82	4641.5
83	2791.5
84	1745.0
85	1095.0
86	695.0
87	463.0
88	335.5
89	227.5
90	156.5
91	122.0
92	81.0
93	45.0
94	27.0
95	13.0
96	7.5
97	6.5
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016375
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.9999999999999996E-6
15-19	0.0
20-24	0.0021
25-29	0.0042450000000000005
30-34	2.226775
35-39	0.012735
40-44	1.73109
45-49	0.0
50-54	8.5E-5
55-59	7.0E-5
60-64	0.031920000000000004
65-69	0.02509
70-74	0.00277
75-79	0.0
80-84	0.004635
85-89	0.0322
90-94	0.042089999999999995
95-99	0.00409
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.05898790747897001
#Duplication Level	Relative count
1	100.0
2	0.03701425048643728
3	0.0010003851482820886
4	5.001925741410443E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	261780	3.0781097	3.8457203	90-94
>>END_MODULE