##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6359915	34.0	31.0	34.0	31.0	34.0
2	32.8787025	34.0	31.0	34.0	31.0	34.0
3	32.8702015	34.0	31.0	34.0	31.0	34.0
4	36.35172475	37.0	37.0	37.0	35.0	37.0
5	36.29754975	37.0	37.0	37.0	35.0	37.0
6	36.27724925	37.0	37.0	37.0	35.0	37.0
7	36.27890425	37.0	37.0	37.0	35.0	37.0
8	36.1803685	37.0	37.0	37.0	35.0	37.0
9	38.01483825	39.0	38.0	39.0	35.0	39.0
10-14	38.27725495	39.4	38.4	39.4	35.2	39.4
15-19	39.426362	41.0	39.0	41.0	36.0	41.0
20-24	39.12194965	40.8	38.8	41.0	35.8	41.0
25-29	38.180785400000005	40.0	37.4	41.0	33.2	41.0
30-34	36.659807	39.2	35.4	41.0	28.6	41.0
35-39	36.977579150000004	38.6	35.0	40.6	32.6	41.0
40-44	35.9561463	36.6	35.0	40.0	31.4	41.0
45-49	35.317892900000004	35.0	35.0	38.6	31.6	41.0
50-54	34.26817255	35.0	34.2	36.2	30.0	39.6
55-59	32.978573350000005	35.0	33.2	35.0	26.0	37.6
60-64	33.1790639	35.0	33.2	35.0	28.6	36.2
65-69	33.01736565	35.0	33.0	35.0	29.0	35.0
70-74	32.456740999999994	35.0	33.0	35.0	27.2	35.0
75-79	31.669356299999997	34.4	32.0	35.0	24.6	35.0
80-84	31.9288724	35.0	32.4	35.0	25.2	35.0
85-89	31.8367503	35.0	33.0	35.0	24.8	35.0
90-94	31.681356299999997	35.0	33.0	35.0	24.8	35.0
95-99	31.3549579	35.0	33.0	35.0	24.0	35.0
100-101	30.324655749999998	34.0	31.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	18.0
4	29.0
5	105.0
6	328.0
7	998.0
8	2274.0
9	3612.0
10	4360.0
11	4892.0
12	5355.0
13	5736.0
14	6124.0
15	6591.0
16	6836.0
17	7371.0
18	8036.0
19	8565.0
20	9428.0
21	10437.0
22	12209.0
23	15126.0
24	19262.0
25	25044.0
26	32232.0
27	40888.0
28	52112.0
29	65591.0
30	83216.0
31	108394.0
32	145904.0
33	219103.0
34	385037.0
35	793374.0
36	1254283.0
37	560063.0
38	88224.0
39	8802.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.28841534963422	23.218617548584067	13.892487338182319	25.600479763599388
2	30.919925	24.0824	19.892599999999998	25.105075
3	28.518349999999998	19.807	21.56525	30.109399999999997
4	30.678775	19.851399999999998	20.035275	29.434549999999998
5	31.160674999999998	20.669575000000002	19.988025	28.181725
6	29.6973	19.097900000000003	17.81185	33.39295
7	32.7753	18.882875	19.171625	29.1702
8	28.675224999999998	19.5323	19.8816	31.910875
9	30.73535	17.867975	19.2575	32.139174999999994
10-14	30.848100000000002	19.24222	19.19462	30.71506
15-19	31.02724	18.810245000000002	18.980375	31.182140000000004
20-24	31.091002619949514	18.85392133911331	18.967633488272927	31.087442552664246
25-29	31.34956600698929	18.941886088272664	18.963046966449106	30.745500938288938
30-34	31.81424484278277	18.629651634426377	18.582513424936053	30.973590097854803
35-39	31.068376893355353	18.83945154771701	18.890258431447116	31.20191312748052
40-44	31.4116590265333	18.88375546332791	18.75277960542929	30.951805904709502
45-49	31.424585	18.699370000000002	18.659354999999998	31.216690000000003
50-54	31.25561969283871	18.786927847581456	18.785492846218204	31.171959613361633
55-59	31.446465	18.960565	18.7222	30.870769999999997
60-64	31.438687759013717	18.70129722064722	18.61251430565035	31.247500714688712
65-69	31.17941215428904	18.76064660238032	18.8012441376964	31.258697105634248
70-74	31.394685	18.9218	18.72477	30.958745
75-79	31.397229999999997	18.750625000000003	18.622685	31.229459999999996
80-84	31.187514999999998	18.738785	18.788885	31.284815
85-89	31.373687694883184	18.916394488568518	18.73654693497127	30.97337088157703
90-94	31.41065567376525	18.633672961182818	18.615806496132684	31.339864868919253
95-99	31.228695000000002	18.699669999999998	18.768099999999997	31.303535
100-101	31.4256125	18.89975	18.749399999999998	30.9252375
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.88889511323361
2	56.025000000000006
3	58.62775
4	60.113324999999996
5	59.3424
6	63.090250000000005
7	61.945499999999996
8	60.5861
9	62.874525000000006
10-14	61.563159999999996
15-19	62.20938
20-24	62.17844517261376
25-29	62.095066945278234
30-34	62.787834940637566
35-39	62.27029002083587
40-44	62.363464931242795
45-49	62.64127499999999
50-54	62.42757930620034
55-59	62.317235000000004
60-64	62.68618847370243
65-69	62.43810925992328
70-74	62.353429999999996
75-79	62.626689999999996
80-84	62.47233
85-89	62.34705857646021
90-94	62.7505205426845
95-99	62.53223
100-101	62.35085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	2.5
3	5.0
4	5.0
5	5.0
6	5.0
7	3.5
8	9.5
9	13.0
10	12.5
11	21.5
12	28.5
13	36.5
14	46.0
15	60.5
16	77.0
17	107.0
18	151.0
19	193.5
20	278.0
21	397.0
22	503.5
23	648.0
24	854.5
25	1076.5
26	1353.0
27	1719.5
28	2180.0
29	2664.5
30	3241.5
31	3884.5
32	4501.0
33	5244.5
34	5982.5
35	6802.5
36	7572.5
37	8258.5
38	9090.0
39	9925.0
40	10853.0
41	11892.0
42	13199.5
43	14942.5
44	17048.5
45	19688.5
46	23278.0
47	27678.5
48	32942.5
49	39840.0
50	47597.5
51	56376.0
52	66623.5
53	77044.5
54	88523.5
55	100555.5
56	112318.5
57	124144.5
58	135915.0
59	147391.5
60	158825.0
61	170191.5
62	179850.0
63	188387.5
64	195494.5
65	200392.0
66	201787.5
67	198991.5
68	192798.0
69	182303.0
70	167478.0
71	149863.5
72	130353.5
73	109679.5
74	89601.5
75	70741.0
76	53861.5
77	39494.5
78	27639.5
79	18627.5
80	11970.5
81	7182.0
82	4117.0
83	2325.5
84	1260.5
85	690.5
86	403.5
87	256.5
88	180.5
89	127.0
90	104.5
91	74.5
92	49.5
93	34.5
94	23.5
95	14.0
96	6.0
97	1.0
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0341
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0018899999999999998
25-29	0.00415
30-34	0.03863
35-39	0.02339
40-44	0.008289999999999999
45-49	0.0
50-54	9.5E-5
55-59	0.0
60-64	0.008915
65-69	0.006245
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0042
90-94	0.0082
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.08549401541892439
#Duplication Level	Relative count
1	100.0
2	0.07706049248660199
3	0.001501178425063675
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	300350	3.286588	3.8683953	95-97
AAAAA	302880	3.1875021	4.1046214	1
>>END_MODULE