##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77198875	34.0	31.0	34.0	31.0	34.0
2	33.005655	34.0	31.0	34.0	31.0	34.0
3	33.0327475	34.0	31.0	34.0	31.0	34.0
4	36.4427175	37.0	37.0	37.0	35.0	37.0
5	36.3812185	37.0	37.0	37.0	35.0	37.0
6	36.356125	37.0	37.0	37.0	35.0	37.0
7	36.36222	37.0	37.0	37.0	35.0	37.0
8	36.345678	37.0	37.0	37.0	35.0	37.0
9	38.156112	39.0	39.0	39.0	37.0	39.0
10-14	38.31914165	39.4	38.8	39.4	36.2	39.4
15-19	39.5160148	41.0	40.0	41.0	37.0	41.0
20-24	39.2892143	41.0	39.0	41.0	36.0	41.0
25-29	38.58814165	40.0	38.4	41.0	34.2	41.0
30-34	37.17047365	39.4	36.0	41.0	31.0	41.0
35-39	36.78146205	38.6	35.0	40.6	31.8	41.0
40-44	35.8169445	36.6	35.0	40.0	30.8	41.0
45-49	35.2309541	35.0	35.0	38.8	31.2	41.0
50-54	34.340138249999995	35.0	34.6	36.4	30.6	40.0
55-59	33.4006921	35.0	33.6	35.0	28.4	38.0
60-64	33.2501337	35.0	33.2	35.0	29.0	36.2
65-69	32.65043965	35.0	33.0	35.0	27.4	35.0
70-74	32.40451155	35.0	33.0	35.0	26.4	35.0
75-79	31.956190399999997	34.4	32.4	35.0	26.4	35.0
80-84	32.2407096	35.0	33.0	35.0	27.0	35.0
85-89	31.914631199999995	35.0	33.0	35.0	26.6	35.0
90-94	31.50939245	35.0	33.0	35.0	24.4	35.0
95-99	30.863990100000002	35.0	32.4	35.0	22.2	35.0
100-101	29.240007499999997	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22.0
3	5.0
4	33.0
5	98.0
6	329.0
7	1063.0
8	2478.0
9	4019.0
10	5166.0
11	5690.0
12	5850.0
13	6211.0
14	6452.0
15	7077.0
16	7778.0
17	8347.0
18	8963.0
19	9512.0
20	10488.0
21	11713.0
22	13339.0
23	16188.0
24	20269.0
25	25338.0
26	31895.0
27	39972.0
28	50310.0
29	62449.0
30	79503.0
31	102696.0
32	138638.0
33	201579.0
34	339485.0
35	751643.0
36	1300983.0
37	613046.0
38	100637.0
39	10712.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.30141404229749	23.0998251795904	13.822565411365604	25.776195366746514
2	31.011925	24.031174999999998	19.83365	25.123250000000002
3	28.354625	19.788025	21.58785	30.2695
4	30.725	19.768774999999998	19.959925000000002	29.5463
5	31.2359	20.61625	20.04675	28.101100000000002
6	29.662274999999998	19.117975	17.823025	33.396724999999996
7	32.78785	18.864800000000002	19.167775000000002	29.179575000000003
8	28.544075000000003	19.4961	19.9698	31.990025
9	30.657899999999998	17.888975	19.2669	32.186225
10-14	30.91296745861583	19.236955198802956	19.17341013208064	30.676667210500568
15-19	31.043864999999997	18.794985	18.98813	31.17302
20-24	30.965399854338965	18.872693996127374	19.01526000834606	31.1466461411876
25-29	31.351831849513946	18.986321367609232	18.875533675468315	30.786313107408507
30-34	31.728102163400994	18.794931627168495	18.566157648106454	30.910808561324053
35-39	31.251896309840436	18.839374111106714	18.84030929473002	31.06842028432283
40-44	31.377520152116656	18.90679854803557	18.78592484930039	30.929756450547387
45-49	31.4316	18.68109	18.65317	31.23414
50-54	31.286580000000004	18.81285	18.776325	31.124245000000002
55-59	31.421335	18.90174	18.730605	30.94632
60-64	31.374526501821386	18.647008473624044	18.622355639780793	31.356109384773777
65-69	31.30560708874432	18.797878777271613	18.769185764505274	31.127328369478796
70-74	31.38878	18.864195	18.695505	31.05152
75-79	31.42997	18.622790000000002	18.612705	31.334535000000002
80-84	31.229590070642338	18.70696667755513	18.75190721010044	31.31153604170209
85-89	31.348554661226856	18.793776827006624	18.717552013409648	31.140116498356875
90-94	31.46279277646162	18.59117248104415	18.57310488382705	31.37292985866718
95-99	31.300602231683293	18.674972294462513	18.736468487488658	31.287956986365533
100-101	31.7189875	18.934575000000002	18.5864625	30.759975
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.077609409044
2	56.135175000000004
3	58.62412500000001
4	60.271300000000004
5	59.336999999999996
6	63.059
7	61.967425
8	60.5341
9	62.844125
10-14	61.5896346691164
15-19	62.216885
20-24	62.112045995526564
25-29	62.13814495692246
30-34	62.63891072472505
35-39	62.32031659416326
40-44	62.30727660266404
45-49	62.66574
50-54	62.410825
55-59	62.367655
60-64	62.73063588659517
65-69	62.43293545822311
70-74	62.44030000000001
75-79	62.76450500000001
80-84	62.541126112344436
85-89	62.48867115958373
90-94	62.835722635128796
95-99	62.588559218048836
100-101	62.4789625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	6.0
2	2.5
3	2.5
4	3.5
5	4.0
6	4.0
7	5.0
8	7.5
9	8.5
10	14.5
11	24.0
12	26.0
13	33.5
14	47.5
15	61.5
16	74.5
17	108.5
18	150.5
19	209.5
20	283.5
21	346.0
22	479.0
23	637.5
24	834.5
25	1120.5
26	1407.5
27	1735.0
28	2166.5
29	2668.0
30	3206.0
31	3910.5
32	4623.5
33	5312.5
34	6104.5
35	6828.0
36	7622.5
37	8449.0
38	9154.0
39	10043.5
40	10868.5
41	11834.0
42	13172.5
43	14863.5
44	17114.5
45	19815.5
46	23373.0
47	27685.5
48	33046.0
49	39703.5
50	47506.5
51	56515.0
52	66655.0
53	77401.0
54	88528.5
55	100286.5
56	112310.5
57	124147.0
58	135343.5
59	146703.0
60	158270.5
61	169376.0
62	179434.5
63	187931.0
64	194668.5
65	199177.0
66	200721.0
67	197966.0
68	191057.5
69	180706.0
70	166973.5
71	149732.5
72	130292.5
73	110410.0
74	90341.0
75	71367.5
76	54688.5
77	40437.0
78	28799.0
79	19554.5
80	12727.5
81	8008.0
82	4839.0
83	2876.0
84	1712.5
85	1070.5
86	684.0
87	468.5
88	335.5
89	228.0
90	170.0
91	130.0
92	96.0
93	64.0
94	37.0
95	20.5
96	14.0
97	8.5
98	3.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025974999999999998
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	1.0499999999999999E-4
15-19	0.0
20-24	7.1E-4
25-29	0.0024300000000000003
30-34	0.043265000000000005
35-39	0.019635
40-44	0.00306
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.011495
65-69	0.0105
70-74	0.0
75-79	0.0
80-84	0.001185
85-89	0.006315
90-94	0.014374999999999999
95-99	0.00194
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.14597956286119299
#Duplication Level	Relative count
1	100.0
2	0.12866984419433652
3	0.0030039652341090238
4	5.00660872351504E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	292120	3.2101784	3.7286968	95-97
AAAAA	295390	3.127664	4.0112	1
>>END_MODULE