##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85474625	34.0	31.0	34.0	31.0	34.0
2	33.0783445	34.0	31.0	34.0	31.0	34.0
3	33.10821325	34.0	33.0	34.0	31.0	34.0
4	36.48390325	37.0	37.0	37.0	35.0	37.0
5	36.419479	37.0	37.0	37.0	35.0	37.0
6	36.38684575	37.0	37.0	37.0	35.0	37.0
7	36.383872	37.0	37.0	37.0	35.0	37.0
8	36.36686225	37.0	37.0	37.0	35.0	37.0
9	38.16762725	39.0	39.0	39.0	37.0	39.0
10-14	38.433941749999995	39.4	39.0	39.4	37.0	39.4
15-19	39.5960514	41.0	40.0	41.0	37.0	41.0
20-24	39.3729096	41.0	39.2	41.0	36.4	41.0
25-29	38.9404547	40.0	38.4	41.0	35.2	41.0
30-34	38.2356486	40.0	37.2	41.0	34.2	41.0
35-39	37.2287906	38.6	35.2	40.6	33.0	41.0
40-44	36.36127524999999	37.0	35.0	40.0	32.4	41.0
45-49	35.6514255	35.2	35.0	39.0	32.8	41.0
50-54	34.7863156	35.0	35.0	36.6	31.6	40.0
55-59	34.13843685	35.0	34.4	35.0	31.0	38.0
60-64	33.643977	35.0	34.0	35.0	30.6	36.2
65-69	33.284540250000006	35.0	33.6	35.0	30.0	35.0
70-74	33.0282278	35.0	33.0	35.0	29.2	35.0
75-79	32.3925536	34.4	32.6	35.0	28.0	35.0
80-84	32.7017439	35.0	33.0	35.0	29.0	35.0
85-89	32.4048585	35.0	33.0	35.0	27.8	35.0
90-94	32.036316899999996	35.0	33.0	35.0	27.0	35.0
95-99	31.5355631	35.0	33.0	35.0	25.6	35.0
100-101	30.347601875000002	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27.0
3	7.0
4	27.0
5	82.0
6	250.0
7	731.0
8	1729.0
9	2538.0
10	3308.0
11	3721.0
12	3900.0
13	4065.0
14	4543.0
15	4972.0
16	5500.0
17	6191.0
18	6634.0
19	7149.0
20	7937.0
21	9049.0
22	10453.0
23	13083.0
24	16816.0
25	21191.0
26	26707.0
27	33670.0
28	42361.0
29	53194.0
30	67317.0
31	86185.0
32	114029.0
33	160371.0
34	263678.0
35	671838.0
36	1472314.0
37	740783.0
38	120797.0
39	12828.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.35360482835609	23.12113018335102	13.808768396669254	25.716496591623635
2	31.007075	24.009675	19.833724999999998	25.149525
3	28.337899999999998	19.796625000000002	21.551275	30.314200000000003
4	30.678725	19.807425000000002	19.972525	29.541325
5	31.19575	20.6037	20.0608	28.139750000000003
6	29.705474999999996	19.0942	17.757575	33.44275
7	32.811725	18.859375	19.150525	29.178375000000003
8	28.577325	19.485525	19.94425	31.992900000000002
9	30.6585	17.832925	19.286175	32.2224
10-14	30.853081706163408	19.23167846335693	19.173928347856695	30.74131148262296
15-19	31.059455000000003	18.7821	18.97345	31.184994999999997
20-24	30.95464	18.849725	18.994025	31.201610000000002
25-29	31.106181174037395	18.979831043732748	18.922997926436267	30.99098985579359
30-34	31.279449222621565	18.740466240963002	18.7158332988152	31.26425123760023
35-39	31.100175972625326	18.814203153264618	18.86363231743165	31.221988556678408
40-44	31.22904029593265	18.85704105554457	18.801390181825855	31.112528466696926
45-49	31.35475	18.65753	18.652214999999998	31.335505
50-54	31.149275	18.764085	18.812704999999998	31.273935
55-59	31.307040000000004	18.811980000000002	18.74792	31.13306
60-64	31.392976150829494	18.631318496574988	18.61236096854061	31.36334438405491
65-69	31.180844993549666	18.72511100013765	18.78786369151085	31.306180314801836
70-74	31.304245	18.821655	18.735950000000003	31.138149999999996
75-79	31.40214	18.621995	18.618904999999998	31.35696
80-84	31.19946628739016	18.727739264869083	18.769976845585266	31.302817602155493
85-89	31.323171933907222	18.797455255455255	18.720776615430538	31.158596195206982
90-94	31.42693129942073	18.595277621516864	18.60332175821416	31.37446932084825
95-99	31.246273103756188	18.672292775567286	18.749281125347135	31.332152995329388
100-101	31.462060279145305	18.862158381009237	18.67199226785309	31.003789071992365
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.07010141997972
2	56.1566
3	58.6521
4	60.22005
5	59.335499999999996
6	63.148225000000004
7	61.990100000000005
8	60.570225
9	62.8809
10-14	61.59439318878638
15-19	62.24444999999999
20-24	62.15625
25-29	62.09717102983099
30-34	62.5437004602218
35-39	62.322164529303734
40-44	62.34156876262957
45-49	62.69025499999999
50-54	62.42321
55-59	62.4401
60-64	62.7563205348844
65-69	62.4870253083515
70-74	62.442395
75-79	62.759100000000004
80-84	62.50228388954565
85-89	62.4817681291142
90-94	62.80140062026898
95-99	62.57842609908558
100-101	62.46584935113767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	1.0
3	0.5
4	2.5
5	3.5
6	4.0
7	3.0
8	3.5
9	7.5
10	9.0
11	11.5
12	21.5
13	33.0
14	42.5
15	58.0
16	75.5
17	101.5
18	140.0
19	191.0
20	260.5
21	344.5
22	472.5
23	620.5
24	820.0
25	1081.0
26	1374.0
27	1733.0
28	2131.0
29	2611.0
30	3225.0
31	3910.0
32	4605.0
33	5268.0
34	5985.5
35	6781.0
36	7452.5
37	8347.5
38	9140.0
39	9755.0
40	10728.0
41	11841.0
42	13247.5
43	14939.5
44	17014.0
45	19749.5
46	23310.5
47	27813.5
48	33284.5
49	39809.0
50	47624.0
51	56837.0
52	66847.0
53	77222.0
54	88513.0
55	100322.0
56	112064.0
57	123721.5
58	135439.0
59	147056.0
60	157974.5
61	168709.5
62	178811.5
63	187643.0
64	194613.5
65	199552.5
66	200862.5
67	197910.5
68	191530.0
69	181284.0
70	167618.0
71	150411.0
72	131396.5
73	111307.0
74	90822.5
75	71926.5
76	54852.5
77	40473.0
78	28843.5
79	19522.0
80	12492.0
81	7570.0
82	4377.5
83	2365.0
84	1266.5
85	711.0
86	411.5
87	254.5
88	159.5
89	102.0
90	77.5
91	51.0
92	34.5
93	25.5
94	14.5
95	6.0
96	2.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0073750000000000005
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	1.9999999999999998E-4
15-19	0.0
20-24	0.0
25-29	0.005484999999999999
30-34	0.052539999999999996
35-39	0.01854
40-44	0.0015699999999999998
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.03971
65-69	0.03616
70-74	0.0
75-79	0.0
80-84	0.006109999999999999
85-89	0.03083
90-94	0.051425
95-99	0.01734
100-101	0.0019000000000000002
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.14048876089912374
#Duplication Level	Relative count
1	100.0
2	0.12967181515508047
3	0.002002653515908579
4	5.006633789771447E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.006633789771447E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	279180	3.0545025	3.6730704	10-14
>>END_MODULE