##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9306845	34.0	31.0	34.0	31.0	34.0
2	33.1417945	34.0	33.0	34.0	31.0	34.0
3	33.1809785	34.0	34.0	34.0	31.0	34.0
4	36.5238565	37.0	37.0	37.0	35.0	37.0
5	36.467858	37.0	37.0	37.0	35.0	37.0
6	36.44991725	37.0	37.0	37.0	35.0	37.0
7	36.4373435	37.0	37.0	37.0	35.0	37.0
8	36.42234675	37.0	37.0	37.0	35.0	37.0
9	38.2344005	39.0	39.0	39.0	37.0	39.0
10-14	38.5018342	39.4	39.2	39.4	37.2	39.4
15-19	39.66977584999999	41.0	40.0	41.0	37.0	41.0
20-24	39.43494215	41.0	39.4	41.0	36.6	41.0
25-29	38.98026455	40.2	38.6	41.0	35.2	41.0
30-34	38.2130181	40.0	37.4	41.0	34.2	41.0
35-39	37.185866	38.6	35.2	40.6	32.8	41.0
40-44	36.3121234	37.0	35.0	40.0	32.4	41.0
45-49	35.626487049999994	35.2	35.0	39.0	32.8	41.0
50-54	34.71396609999999	35.0	35.0	36.6	31.4	40.0
55-59	34.015916100000005	35.0	34.2	35.0	31.0	38.0
60-64	33.51300725	35.0	34.0	35.0	30.2	36.2
65-69	33.1038346	35.0	33.2	35.0	29.4	35.0
70-74	32.82229554999999	35.0	33.0	35.0	29.0	35.0
75-79	32.1370773	34.4	32.6	35.0	27.4	35.0
80-84	32.42358365	35.0	33.0	35.0	28.0	35.0
85-89	32.075730750000005	35.0	33.0	35.0	27.0	35.0
90-94	31.6317366	35.0	33.0	35.0	25.2	35.0
95-99	31.04363115	35.0	32.6	35.0	23.8	35.0
100-101	29.631221625000002	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24.0
3	9.0
4	22.0
5	96.0
6	309.0
7	870.0
8	1852.0
9	3121.0
10	4009.0
11	4543.0
12	4788.0
13	4852.0
14	5486.0
15	5797.0
16	6518.0
17	7274.0
18	7664.0
19	8286.0
20	9154.0
21	10592.0
22	11984.0
23	14536.0
24	18022.0
25	22533.0
26	27999.0
27	34652.0
28	43171.0
29	53864.0
30	68029.0
31	87335.0
32	116204.0
33	165048.0
34	278550.0
35	701260.0
36	1437819.0
37	705931.0
38	115740.0
39	12030.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.32329472580503	23.073068229472604	13.787581324248974	25.81605572047339
2	31.045250000000003	23.974149999999998	19.783275	25.197325
3	28.334274999999998	19.76445	21.5589	30.342375
4	30.716450000000002	19.72325	19.878625	29.681675000000002
5	31.248725	20.65625	20.013775	28.08125
6	29.667949999999998	19.12865	17.782025	33.421375
7	32.830075	18.8513	19.169800000000002	29.148825000000002
8	28.570024999999998	19.508300000000002	19.9251	31.996575
9	30.713425	17.862025	19.232625	32.191925
10-14	30.840278176389447	19.236626931277705	19.175506845709585	30.747588046623264
15-19	31.048019999999998	18.79254	18.974815	31.184625
20-24	30.954369999999997	18.86025	18.987285	31.198095
25-29	31.105741131153586	18.972534315916995	18.91448713044046	31.00723742248896
30-34	31.2934900727061	18.725722107816356	18.734782852328376	31.246004967149172
35-39	31.082359348194466	18.82021588326531	18.870779845727657	31.226644922812568
40-44	31.233643439225347	18.862128380439227	18.783681889955908	31.12054629037952
45-49	31.355875	18.6602	18.641705	31.342219999999998
50-54	31.152466011432967	18.721845778428648	18.834141412898983	31.291546797239405
55-59	31.279780000000002	18.796395	18.75815	31.165674999999997
60-64	31.402017467080274	18.6185915947242	18.614364736174497	31.365026202021028
65-69	31.17254021233622	18.729573195650833	18.788058045863686	31.30982854614926
70-74	31.329425	18.817405	18.70999	31.143179999999997
75-79	31.424210000000002	18.62309	18.59422	31.35848
80-84	31.21677665108643	18.70019497965395	18.75941594912767	31.323612420131948
85-89	31.361446572623507	18.773310496995478	18.6927813929199	31.172461537461118
90-94	31.465744118578332	18.56977396948141	18.58363700519006	31.380844906750198
95-99	31.289376707908794	18.651870654474127	18.736457448261547	31.322295189355536
100-101	31.542304739327843	18.791356021231685	18.635300988456876	31.031038250983595
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.13935044627842
2	56.242574999999995
3	58.676649999999995
4	60.398125
5	59.329975000000005
6	63.089324999999995
7	61.9789
8	60.5666
9	62.905350000000006
10-14	61.58786622301271
15-19	62.232645
20-24	62.152465
25-29	62.11297855364254
30-34	62.53949503985526
35-39	62.30900427100704
40-44	62.35418972960486
45-49	62.698094999999995
50-54	62.44401280867237
55-59	62.445455
60-64	62.767043669101305
65-69	62.482368758485485
70-74	62.472605
75-79	62.782689999999995
80-84	62.540389071218385
85-89	62.533908110084624
90-94	62.846589025328534
95-99	62.61167189726433
100-101	62.573342990311446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	2.0
3	2.5
4	1.5
5	2.5
6	2.0
7	1.5
8	4.0
9	7.5
10	10.5
11	10.0
12	13.0
13	32.0
14	50.0
15	60.0
16	74.0
17	100.0
18	146.5
19	201.0
20	266.5
21	354.0
22	454.5
23	621.0
24	831.0
25	1092.5
26	1392.5
27	1729.0
28	2197.5
29	2721.0
30	3225.5
31	3908.0
32	4608.0
33	5245.5
34	5996.0
35	6774.0
36	7559.5
37	8284.5
38	9037.5
39	9777.5
40	10706.5
41	11807.5
42	13045.5
43	14758.0
44	16979.0
45	19714.5
46	23250.0
47	27943.5
48	33394.0
49	39750.0
50	47750.0
51	56811.0
52	66819.5
53	77644.0
54	88832.5
55	100248.0
56	111937.0
57	123907.0
58	135567.5
59	146819.0
60	157687.5
61	168739.0
62	178988.0
63	187566.5
64	194662.5
65	199116.0
66	200089.5
67	197523.0
68	191292.0
69	180917.5
70	166604.0
71	149602.5
72	130413.0
73	110369.0
74	90889.5
75	72378.0
76	55446.0
77	40848.0
78	29026.0
79	19841.0
80	12999.0
81	8073.5
82	4835.0
83	2860.5
84	1703.5
85	1016.0
86	642.5
87	419.0
88	286.0
89	206.0
90	150.0
91	117.0
92	81.5
93	48.5
94	30.5
95	23.5
96	16.5
97	7.5
98	3.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025974999999999998
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	1.4E-4
15-19	0.0
20-24	0.0
25-29	0.003765
30-34	0.0634
35-39	0.017725
40-44	0.0019000000000000002
45-49	0.0
50-54	5.65E-4
55-59	0.0
60-64	0.04397
65-69	0.04249
70-74	0.0
75-79	0.0
80-84	0.01008
85-89	0.04235
90-94	0.057965
95-99	0.025765
100-101	0.0032249999999999996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.13598436179839773
#Duplication Level	Relative count
1	100.0
2	0.12265270261475537
3	5.006232759785933E-4
4	5.006232759785933E-4
5	5.006232759785933E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.006232759785933E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	280695	3.0807538	3.6216156	10-14
AAAAA	282525	3.0182729	3.8649364	1
>>END_MODULE