##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93746675	34.0	31.0	34.0	31.0	34.0
2	33.15251375	34.0	33.0	34.0	31.0	34.0
3	33.18313275	34.0	34.0	34.0	31.0	34.0
4	36.532631	37.0	37.0	37.0	35.0	37.0
5	36.47699525	37.0	37.0	37.0	35.0	37.0
6	36.451798	37.0	37.0	37.0	35.0	37.0
7	36.443437	37.0	37.0	37.0	35.0	37.0
8	36.423118	37.0	37.0	37.0	35.0	37.0
9	38.24100725	39.0	39.0	39.0	37.0	39.0
10-14	38.515671149999996	39.4	39.2	39.4	37.2	39.4
15-19	39.68584085	41.0	40.0	41.0	37.2	41.0
20-24	39.458416799999995	41.0	39.6	41.0	36.6	41.0
25-29	39.0037936	40.2	38.6	41.0	35.2	41.0
30-34	38.24988925	40.0	37.4	41.0	34.2	41.0
35-39	37.2172037	38.8	35.2	40.6	32.8	41.0
40-44	36.36371989999999	37.0	35.0	40.0	32.6	41.0
45-49	35.660706700000006	35.2	35.0	39.0	32.8	41.0
50-54	34.7613798	35.0	35.0	36.6	31.6	40.0
55-59	34.0632713	35.0	34.4	35.0	31.0	38.0
60-64	33.5474212	35.0	34.0	35.0	30.4	36.4
65-69	33.1513452	35.0	33.4	35.0	29.6	35.0
70-74	32.8882112	35.0	33.0	35.0	29.0	35.0
75-79	32.182482549999996	34.4	32.6	35.0	27.4	35.0
80-84	32.4742525	35.0	33.0	35.0	28.2	35.0
85-89	32.132754850000005	35.0	33.0	35.0	27.0	35.0
90-94	31.728603100000004	35.0	33.0	35.0	25.8	35.0
95-99	31.1580791	35.0	32.8	35.0	23.8	35.0
100-101	29.85703625	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27.0
3	5.0
4	40.0
5	97.0
6	304.0
7	792.0
8	1830.0
9	2814.0
10	3727.0
11	4320.0
12	4558.0
13	4782.0
14	5270.0
15	5671.0
16	6148.0
17	7112.0
18	7847.0
19	8356.0
20	9294.0
21	10217.0
22	11986.0
23	13971.0
24	17371.0
25	21658.0
26	27648.0
27	34005.0
28	42821.0
29	52747.0
30	66397.0
31	85673.0
32	114609.0
33	162201.0
34	274563.0
35	684510.0
36	1443445.0
37	729448.0
38	120676.0
39	13041.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.364910783983774	23.027488664532676	13.801190880418385	25.80640967106517
2	31.061625	23.96415	19.795625	25.1786
3	28.346125	19.723325	21.5581	30.37245
4	30.754825000000004	19.755125	19.903399999999998	29.58665
5	31.269999999999996	20.595325	20.03075	28.103925
6	29.675425	19.0964	17.817425	33.41075
7	32.8244	18.836375	19.149525	29.189700000000002
8	28.59135	19.510625	19.960275	31.937749999999998
9	30.698175	17.8454	19.2712	32.185225
10-14	30.84548	19.220760000000002	19.191805000000002	30.741954999999997
15-19	31.045045	18.78892	18.970509999999997	31.195525000000004
20-24	30.941403854476018	18.862357320911915	19.008063304427306	31.188175520184764
25-29	31.10936520930255	18.9828004727235	18.941955170468262	30.965879147505692
30-34	31.26491427038823	18.741318017566996	18.739651737447293	31.254115974597486
35-39	31.077786884479362	18.83195375055829	18.88163803406667	31.20862133089568
40-44	31.227704118597366	18.88493217672003	18.804726254351927	31.08263745033068
45-49	31.35349	18.663555000000002	18.64807	31.334885
50-54	31.140445153645473	18.746242182051166	18.82884730182859	31.284465362474776
55-59	31.275344999999998	18.820195000000002	18.759124999999997	31.145335000000003
60-64	31.364782059753743	18.63862893011552	18.63596315650802	31.36062585362272
65-69	31.187324233760982	18.730672778619343	18.79188867160362	31.290114316016055
70-74	31.308615	18.814105	18.724674999999998	31.152605
75-79	31.394270000000002	18.627535	18.600755	31.37744
80-84	31.224870313352664	18.713976104923635	18.768921569250065	31.29223201247364
85-89	31.33008702200368	18.78315942070158	18.743351610548274	31.14340194674646
90-94	31.45086735792794	18.58569251828569	18.57481309144691	31.38862703233946
95-99	31.279747644266102	18.661620912138243	18.7344439153863	31.32418752820935
100-101	31.582636222021275	18.8335430438162	18.59612811031313	30.987692623849394
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.17132045504894
2	56.240225
3	58.718574999999994
4	60.341475
5	59.37392500000001
6	63.086175
7	62.014100000000006
8	60.5291
9	62.8834
10-14	61.587435
15-19	62.24057
20-24	62.12957937466078
25-29	62.07524435680825
30-34	62.51903024498571
35-39	62.28640821537505
40-44	62.31034156892804
45-49	62.68837499999999
50-54	62.42491051612025
55-59	62.42068
60-64	62.725407913376465
65-69	62.47743854977704
70-74	62.46122
75-79	62.771710000000006
80-84	62.5171023258263
85-89	62.47348896875015
90-94	62.839494390267404
95-99	62.60393517247545
100-101	62.57032884587067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	5.0
2	1.0
3	0.0
4	2.0
5	3.5
6	3.5
7	3.5
8	4.5
9	7.0
10	9.5
11	12.5
12	17.5
13	26.5
14	33.0
15	53.0
16	84.0
17	107.5
18	147.5
19	217.0
20	297.0
21	377.5
22	491.0
23	667.5
24	833.0
25	1051.5
26	1362.0
27	1757.0
28	2188.5
29	2625.0
30	3200.5
31	3896.5
32	4598.0
33	5337.0
34	6031.0
35	6767.5
36	7636.0
37	8436.5
38	9141.0
39	9951.0
40	10857.0
41	11834.0
42	13181.5
43	14884.5
44	16942.0
45	19755.0
46	23350.5
47	27884.0
48	33294.0
49	39815.5
50	47579.0
51	56746.0
52	66969.0
53	77549.5
54	89038.0
55	100650.5
56	112297.0
57	123604.5
58	134956.5
59	147095.0
60	158681.0
61	168977.5
62	178878.5
63	187751.5
64	194134.0
65	198438.5
66	200191.5
67	197738.5
68	190930.5
69	180757.0
70	166986.0
71	149801.5
72	130552.0
73	110789.5
74	90873.0
75	71977.5
76	55365.0
77	40721.5
78	28744.5
79	19616.0
80	12729.0
81	7929.0
82	4707.0
83	2690.0
84	1567.0
85	948.0
86	594.5
87	379.0
88	267.5
89	196.5
90	132.0
91	94.5
92	73.0
93	46.0
94	30.0
95	20.0
96	9.0
97	5.5
98	5.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01515
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	6.749999999999999E-4
25-29	7.4E-4
30-34	0.076825
35-39	0.018685
40-44	0.00115
45-49	0.0
50-54	1.45E-4
55-59	0.0
60-64	0.02902
65-69	0.03413
70-74	0.0
75-79	0.0
80-84	0.009945
85-89	0.03218
90-94	0.040690000000000004
95-99	0.01099
100-101	0.001025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.14748893832962495
#Duplication Level	Relative count
1	100.0
2	0.1381934708592029
3	0.0015021029441217705
4	0.0
5	5.007009813739235E-4
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	279670	3.051783	3.661185	10-14
>>END_MODULE