##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73892975	34.0	31.0	34.0	31.0	34.0
2	32.98512	34.0	31.0	34.0	31.0	34.0
3	33.04056925	34.0	31.0	34.0	31.0	34.0
4	36.4201875	37.0	37.0	37.0	35.0	37.0
5	36.34439475	37.0	37.0	37.0	35.0	37.0
6	36.31237425	37.0	37.0	37.0	35.0	37.0
7	36.31023625	37.0	37.0	37.0	35.0	37.0
8	36.29685825	37.0	37.0	37.0	35.0	37.0
9	38.08118	39.0	39.0	39.0	37.0	39.0
10-14	38.34659845	39.4	38.4	39.4	35.4	39.4
15-19	39.48920795	41.0	39.6	41.0	37.0	41.0
20-24	39.273144450000004	40.8	39.0	41.0	35.8	41.0
25-29	38.8201124	40.0	38.2	41.0	35.0	41.0
30-34	38.095112650000004	40.0	37.0	41.0	33.6	41.0
35-39	37.09591214999999	38.6	35.2	40.6	32.6	41.0
40-44	36.21722925	36.8	35.0	40.0	32.0	41.0
45-49	35.5014674	35.0	35.0	38.8	32.0	41.0
50-54	34.59027805	35.0	34.6	36.6	31.0	40.0
55-59	33.925723700000006	35.0	34.0	35.0	30.4	38.0
60-64	33.4394616	35.0	33.8	35.0	30.0	36.2
65-69	33.0362009	35.0	33.0	35.0	29.0	35.0
70-74	32.76011505	35.0	33.0	35.0	29.0	35.0
75-79	32.09528805	34.4	32.4	35.0	26.8	35.0
80-84	32.40226525	35.0	33.0	35.0	27.2	35.0
85-89	32.08931665	35.0	33.0	35.0	27.0	35.0
90-94	31.65832765	35.0	33.0	35.0	25.2	35.0
95-99	31.13041285	35.0	32.8	35.0	23.8	35.0
100-101	29.891600875	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25.0
3	12.0
4	29.0
5	86.0
6	313.0
7	763.0
8	1944.0
9	3160.0
10	3909.0
11	4512.0
12	4838.0
13	4965.0
14	5469.0
15	5701.0
16	6593.0
17	7099.0
18	7961.0
19	8545.0
20	9324.0
21	10489.0
22	12219.0
23	15215.0
24	18804.0
25	23703.0
26	30335.0
27	37967.0
28	47721.0
29	59460.0
30	75440.0
31	96341.0
32	126175.0
33	175476.0
34	282246.0
35	672748.0
36	1408725.0
37	705407.0
38	114275.0
39	11982.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.24526890904475	23.166307554363932	13.864744509208323	25.72367902738299
2	30.9563	24.04805	19.870775	25.124875000000003
3	28.281875	19.820275000000002	21.5902	30.307650000000002
4	30.705675	19.83015	19.936575	29.5276
5	31.186275000000002	20.659525000000002	20.037025	28.117175
6	29.68025	19.0884	17.81035	33.421
7	32.77585	18.8856	19.179375	29.159174999999998
8	28.588599999999996	19.55365	19.94405	31.9137
9	30.695275	17.860875	19.2517	32.192150000000005
10-14	30.834969967089325	19.23951793142093	19.197192704980974	30.72831939650877
15-19	31.04185	18.80179	18.962195	31.194165
20-24	30.93719645119746	18.881969900508867	18.990980592726764	31.189853055566903
25-29	31.10387531813817	18.99122488157225	18.93162475641199	30.973275043877592
30-34	31.26103011797474	18.750570264602775	18.741641121480367	31.246758495942117
35-39	31.05793395082863	18.82011196963152	18.902774818469016	31.21917926107084
40-44	31.22693955490244	18.878538551596673	18.81316817571701	31.081353717783877
45-49	31.340154999999996	18.657294999999998	18.6676	31.33495
50-54	31.146424137329287	18.753532010982426	18.82616980753759	31.273874044150702
55-59	31.274705000000004	18.83311	18.768425	31.12376
60-64	31.386309480648393	18.642079706756746	18.620976594047622	31.350634218547235
65-69	31.181722233376934	18.729989970762436	18.790430442074086	31.297857353786547
70-74	31.29591	18.820045	18.728369999999998	31.155675
75-79	31.393379999999997	18.624945	18.61782	31.363855
80-84	31.18719951069671	18.733896733484514	18.77560371873701	31.303300037081765
85-89	31.331016513032928	18.798228855139225	18.707216272649692	31.163538359178155
90-94	31.441347970305827	18.60208164066708	18.573400293484948	31.383170095542145
95-99	31.26685656391061	18.677015309752754	18.75031939279018	31.30580873354646
100-101	31.53336171674472	18.853051845892576	18.64737628028477	30.966210157077935
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.968947936427746
2	56.081175
3	58.589524999999995
4	60.233275
5	59.303450000000005
6	63.10125
7	61.935024999999996
8	60.5023
9	62.88742499999999
10-14	61.563289363598095
15-19	62.236015
20-24	62.127049506764365
25-29	62.077150362015765
30-34	62.50778861391686
35-39	62.27711321189947
40-44	62.30829327268632
45-49	62.675104999999995
50-54	62.420298181479986
55-59	62.398465
60-64	62.736943699195635
65-69	62.47957958716348
70-74	62.451585
75-79	62.757235
80-84	62.49049954777848
85-89	62.49455487221108
90-94	62.82451806584797
95-99	62.572665297457064
100-101	62.49957187382266
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	5.5
2	1.5
3	2.0
4	2.5
5	3.0
6	3.5
7	3.5
8	4.0
9	5.5
10	8.5
11	14.0
12	22.5
13	31.5
14	43.0
15	56.5
16	75.5
17	100.5
18	132.0
19	191.0
20	274.0
21	358.0
22	452.0
23	611.5
24	822.0
25	1068.5
26	1368.0
27	1718.5
28	2148.0
29	2698.5
30	3294.0
31	3868.0
32	4537.0
33	5300.0
34	6005.5
35	6736.5
36	7567.0
37	8386.0
38	9160.0
39	9880.0
40	10821.5
41	11972.0
42	13211.5
43	14737.0
44	16870.0
45	19677.0
46	23172.5
47	27821.5
48	33401.0
49	39954.0
50	47933.5
51	57215.0
52	66904.0
53	77427.5
54	89115.5
55	100593.5
56	112458.5
57	124277.0
58	135581.0
59	146940.5
60	158049.5
61	168627.5
62	178790.5
63	187722.5
64	194745.5
65	199300.0
66	200521.5
67	198256.0
68	191611.5
69	181090.0
70	167234.0
71	150321.5
72	130962.5
73	110690.5
74	90603.5
75	71458.5
76	54528.5
77	40085.5
78	28260.5
79	19067.0
80	12388.0
81	7710.0
82	4533.0
83	2534.0
84	1418.5
85	850.0
86	510.0
87	340.0
88	262.5
89	170.5
90	113.0
91	77.5
92	45.0
93	30.0
94	22.5
95	13.5
96	4.5
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042874999999999996
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	5.35E-4
15-19	0.0
20-24	6.35E-4
25-29	2.0999999999999998E-4
30-34	0.0464
35-39	0.009495
40-44	5.75E-4
45-49	0.0
50-54	0.00385
55-59	0.0
60-64	0.014750000000000001
65-69	0.017325
70-74	0.0
75-79	0.0
80-84	0.0047599999999999995
85-89	0.013825
90-94	0.02213
95-99	0.00557
100-101	2.75E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.146488280937518
#Duplication Level	Relative count
1	100.0
2	0.13468654085908985
3	0.002503467302213566
4	0.0010013869208854262
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.006934604427131E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	282145	3.077374	3.6177578	9
AAAAA	284915	3.0123436	3.8723636	1
>>END_MODULE