##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002007_ItsyBitsy_ATTCCT_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	62	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7713785	34.0	31.0	34.0	31.0	34.0
2	33.01090025	34.0	31.0	34.0	31.0	34.0
3	33.037923	34.0	31.0	34.0	31.0	34.0
4	36.42971725	37.0	37.0	37.0	35.0	37.0
5	36.36682025	37.0	37.0	37.0	35.0	37.0
6	36.3356145	37.0	37.0	37.0	35.0	37.0
7	36.320281	37.0	37.0	37.0	35.0	37.0
8	36.31746075	37.0	37.0	37.0	35.0	37.0
9	38.112006	39.0	39.0	39.0	37.0	39.0
10-14	38.3720364	39.4	38.6	39.4	36.2	39.4
15-19	39.5153622	41.0	40.0	41.0	37.0	41.0
20-24	39.29128325	40.8	39.0	41.0	36.0	41.0
25-29	38.84089555	40.0	38.4	41.0	35.0	41.0
30-34	38.084887099999996	40.0	37.0	41.0	33.4	41.0
35-39	37.0753609	38.6	35.2	40.6	32.6	41.0
40-44	36.2077406	36.8	35.0	40.0	31.8	41.0
45-49	35.45073615	35.0	35.0	39.0	31.8	41.0
50-54	34.548868600000006	35.0	34.8	36.6	31.0	40.0
55-59	33.87309115000001	35.0	34.0	35.0	30.4	38.0
60-64	33.3462496	35.0	33.8	35.0	29.8	36.2
65-69	32.94636345	35.0	33.0	35.0	29.0	35.0
70-74	32.664740949999995	35.0	33.0	35.0	28.8	35.0
75-79	31.940243350000003	34.4	32.4	35.0	26.4	35.0
80-84	32.23777775000001	35.0	33.0	35.0	27.0	35.0
85-89	31.88232175	35.0	33.0	35.0	26.4	35.0
90-94	31.48354475	35.0	33.0	35.0	24.4	35.0
95-99	30.90191375	35.0	32.4	35.0	22.6	35.0
100-101	29.739563125	34.0	30.5	35.0	10.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35.0
3	9.0
4	31.0
5	116.0
6	335.0
7	899.0
8	2177.0
9	3584.0
10	4455.0
11	5150.0
12	5441.0
13	5821.0
14	6084.0
15	6528.0
16	7315.0
17	8217.0
18	8966.0
19	9556.0
20	10340.0
21	11556.0
22	13464.0
23	16187.0
24	19854.0
25	24857.0
26	31463.0
27	38525.0
28	48498.0
29	60203.0
30	75399.0
31	96599.0
32	127257.0
33	175959.0
34	282651.0
35	659485.0
36	1375967.0
37	720675.0
38	122188.0
39	14125.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.2257716438081	23.134819463075328	13.84237733401601	25.79703155910056
2	31.029075	24.05525	19.8105	25.105175000000003
3	28.306575000000002	19.832250000000002	21.5822	30.278975000000003
4	30.71325	19.788575	19.95445	29.543725
5	31.256024999999998	20.622475	20.0623	28.0592
6	29.6964	19.1228	17.793275	33.387525000000004
7	32.77895	18.891425	19.1722	29.157424999999996
8	28.5722	19.52595	19.936925	31.964925
9	30.703725	17.85825	19.300175	32.13785
10-14	30.805265449477808	19.27745469941846	19.215329587593256	30.701950263510476
15-19	31.02728	18.812175	18.990135	31.170409999999997
20-24	30.928854752974903	18.867263106262616	19.03800489905144	31.165877241711037
25-29	31.088054999999997	18.99096	18.96155	30.959435000000003
30-34	31.251521385928505	18.75453790004604	18.755598212573155	31.238342501452305
35-39	31.10575965583301	18.83209513774325	18.877902132637878	31.18424307378586
40-44	31.22063	18.893225	18.816005	31.070140000000002
45-49	31.351125000000003	18.670835	18.65983	31.31821
50-54	31.151962147063728	18.768827567779244	18.839127820860156	31.24008246429687
55-59	31.266090000000002	18.83583	18.778425000000002	31.119655
60-64	31.395057396862075	18.623050398981604	18.620795050755838	31.361097153400486
65-69	31.191144946318726	18.7555225938444	18.804751787396295	31.24858067244058
70-74	31.29766	18.816405	18.75563	31.130305000000003
75-79	31.404654999999998	18.62724	18.611050000000002	31.357055
80-84	31.227886096178846	18.712148444525738	18.776961643057085	31.28300381623833
85-89	31.3326337712464	18.794298542321172	18.72895462941145	31.144113057020977
90-94	31.4558193921776	18.588083176876616	18.595559891661154	31.360537539284632
95-99	31.306880340380218	18.68264379117029	18.71335633724036	31.297119531209137
100-101	31.551434565780223	18.843932283255114	18.636380000180004	30.96825315078466
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	63.02280320290866
2	56.134249999999994
3	58.58555
4	60.256975000000004
5	59.315225000000005
6	63.083924999999994
7	61.936375
8	60.537125
9	62.841575000000006
10-14	61.507215712988284
15-19	62.19769000000001
20-24	62.09473199468595
25-29	62.04749
30-34	62.48986388738081
35-39	62.29000272961888
40-44	62.290769999999995
45-49	62.669335000000004
50-54	62.392044611360596
55-59	62.385745
60-64	62.75615455026256
65-69	62.4397256187593
70-74	62.42796499999999
75-79	62.76171
80-84	62.51088991241718
85-89	62.47674682826737
90-94	62.81635693146224
95-99	62.60399987158935
100-101	62.51968771656489
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.0
2	2.5
3	4.0
4	4.5
5	3.0
6	1.5
7	2.0
8	3.5
9	7.5
10	9.0
11	11.0
12	22.0
13	32.0
14	39.5
15	58.5
16	83.5
17	116.5
18	156.5
19	191.5
20	268.0
21	378.0
22	492.5
23	655.0
24	859.5
25	1120.5
26	1455.5
27	1798.0
28	2223.0
29	2731.5
30	3289.0
31	3960.5
32	4679.0
33	5397.5
34	6143.0
35	6962.0
36	7674.0
37	8352.5
38	9130.5
39	9991.0
40	10931.5
41	12091.5
42	13423.0
43	14905.0
44	17042.5
45	19902.0
46	23414.0
47	27868.5
48	33345.0
49	39930.0
50	47848.0
51	56891.0
52	66696.0
53	77511.5
54	89124.0
55	100616.0
56	112252.0
57	124233.0
58	135789.0
59	147023.0
60	158094.0
61	169311.0
62	179330.0
63	187438.0
64	194253.0
65	199053.5
66	200121.5
67	197194.5
68	191074.5
69	180726.5
70	166552.5
71	149346.5
72	130050.5
73	110070.0
74	90243.5
75	71626.0
76	54820.0
77	40260.0
78	28594.0
79	19535.5
80	12734.0
81	7984.5
82	4845.5
83	2884.0
84	1693.5
85	1004.5
86	629.5
87	441.5
88	297.0
89	196.5
90	146.0
91	112.0
92	68.0
93	36.0
94	32.5
95	25.0
96	14.0
97	7.5
98	3.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050225
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	1.7999999999999998E-4
15-19	0.0
20-24	0.00105
25-29	0.0
30-34	0.029474999999999998
35-39	0.004355
40-44	0.0
45-49	0.0
50-54	3.5999999999999997E-4
55-59	0.0
60-64	0.01544
65-69	0.018675
70-74	0.0
75-79	0.0
80-84	0.004935
85-89	0.013640000000000001
90-94	0.022935
95-99	0.008289999999999999
100-101	0.0011
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.15448918575699208
#Duplication Level	Relative count
1	100.0
2	0.14271049798452717
3	0.004005908715355149
4	5.007385894193936E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.007385894193936E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	282545	3.086977	3.6239533	9
>>END_MODULE