##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_R2_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	65184661	
Filtered Sequences	0	
Sequence length	30-101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.751156058018005	34.0	31.0	34.0	31.0	34.0
2	32.851653090594425	34.0	31.0	34.0	31.0	34.0
3	32.89855512173332	34.0	31.0	34.0	31.0	34.0
4	36.267073905623285	37.0	37.0	37.0	35.0	37.0
5	36.26522353165264	37.0	37.0	37.0	35.0	37.0
6	36.267324808209096	37.0	37.0	37.0	35.0	37.0
7	36.26833645725334	37.0	37.0	37.0	35.0	37.0
8	36.253631479344506	37.0	37.0	37.0	35.0	37.0
9	38.023417303650625	39.0	39.0	39.0	37.0	39.0
10-14	38.29828352716294	39.4	39.0	39.4	35.8	39.4
15-19	39.466115045071724	41.0	40.0	41.0	36.8	41.0
20-24	39.23415270350182	41.0	39.0	41.0	35.8	41.0
25-29	38.946727537633436	40.2	38.6	41.0	35.0	41.0
30-34	38.38178824759119	40.0	37.4	41.0	34.4	41.0
35-39	37.480263292055	39.0	35.4	41.0	33.0	41.0
40-44	36.60882499490607	37.2	35.0	40.0	32.8	41.0
45-49	35.68749719751554	35.2	35.0	39.0	31.6	41.0
50-54	34.39745082707416	35.0	34.0	36.2	30.2	39.2
55-59	34.521460439306296	35.0	35.0	35.2	31.4	38.2
60-64	34.17085163674487	35.0	35.0	35.0	31.0	36.4
65-69	33.91352176764779	35.0	34.2	35.0	31.0	35.2
70-74	33.73931914818213	35.0	34.0	35.0	31.0	35.0
75-79	33.58666763648932	35.0	34.0	35.0	30.4	35.0
80-84	33.46881750053503	35.0	33.6	35.0	30.0	35.0
85-89	33.37203167802549	35.0	33.0	35.0	29.8	35.0
90-94	33.27967290210182	35.0	33.0	35.0	29.0	35.0
95-99	33.312043345541085	35.0	33.0	35.0	29.2	35.0
100-101	33.020254554759376	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	32.0
17	146.0
18	700.0
19	3866.0
20	12735.0
21	32361.0
22	67803.0
23	123427.0
24	200917.0
25	301610.0
26	421766.0
27	564036.0
28	725557.0
29	916320.0
30	1155623.0
31	1475375.0
32	1945517.0
33	2740411.0
34	4474503.0
35	1.0580284E7
36	2.3559964E7
37	1.3725912E7
38	1952747.0
39	201699.0
40	1347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.28158284047837	24.42060870731536	13.570718730898976	23.727089721307287
2	30.441344207277787	24.85865888730992	19.145203072230576	25.554793833181716
3	29.367409692134324	19.96333313688928	21.450005983079308	29.21925118789709
4	30.59621087561387	19.876067084510662	19.93224929421617	29.595472745659297
5	31.09684608274868	21.09204881808721	20.112793339506776	27.698311759657336
6	29.32821554027371	19.471172637491733	17.552342453488066	33.64826936874649
7	32.54226153229502	19.020685966472417	19.494064176074957	28.94298832515761
8	28.33240769842161	19.865570982599444	19.905452632709203	31.896568686269745
9	30.513132725674406	18.22865816975489	19.278858785598878	31.97935031897183
10-14	30.425955878184745	19.675854246349566	19.541507235658546	30.356682639807136
15-19	30.664028650090803	19.206771670716112	19.366615761459677	30.76258391773341
20-24	30.59330118401323	19.250040699206384	19.360125079803677	30.796533036976715
25-29	30.712260426259448	19.38187960292474	19.295992992396826	30.60986697841898
30-34	30.87081641025832	19.144458019531914	19.105087045274214	30.879638524935547
35-39	30.716129156206673	19.21434416075767	19.234587685028046	30.834938998007612
40-44	30.8232258438839	19.289967472418635	19.175798734125316	30.71100794957215
45-49	30.9421542620014	19.077730500579932	19.04399813774269	30.93611709967598
50-54	30.740835977392365	19.157363270401895	19.200635635955933	30.901165116249814
55-59	30.836358111256505	19.255273305710812	19.171732073363117	30.73663650966956
60-64	30.937270105222403	19.0742602013906	19.033715933434657	30.954753759952343
65-69	30.72168132404555	19.17338049702953	19.210881690938585	30.894056487986333
70-74	30.81558835867745	19.281708125571186	19.177694858741702	30.72500865700966
75-79	30.909739625809486	19.10719411043257	19.060155815046215	30.922910448711725
80-84	30.690038933242292	19.204556447463315	19.230355468321736	30.875049150972657
85-89	30.763222468589635	19.320677783100834	19.218712214362604	30.69738753394693
90-94	30.827063617947424	19.16192061422916	19.11298616184978	30.898029605973637
95-99	30.558748058169133	19.265850656359607	19.32463085080499	30.850770434666273
100-101	30.903684521806923	19.357095566954456	19.147594966836305	30.59162494440232
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.00867256178566
2	55.9961380404595
3	58.58666088003142
4	60.19168362127317
5	58.79515784240601
6	62.9764849090202
7	61.48524985745263
8	60.22897638469136
9	62.49248304464623
10-14	60.782638517991884
15-19	61.426612567824215
20-24	61.38983422098995
25-29	61.322127404678426
30-34	61.75045493519388
35-39	61.551068154214285
40-44	61.53423379345605
45-49	61.878271361677385
50-54	61.64200109364217
55-59	61.57299462092607
60-64	61.892023865174735
65-69	61.61573781203189
70-74	61.54059701568711
75-79	61.83265007452121
80-84	61.56508808421495
85-89	61.46061000253656
90-94	61.72509322392106
95-99	61.4095184928354
100-101	61.495309466209235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	364.0
1	257.0
2	116.0
3	85.0
4	90.5
5	105.0
6	131.5
7	161.5
8	193.0
9	245.0
10	335.0
11	450.5
12	603.0
13	784.5
14	997.5
15	1293.5
16	1663.5
17	2147.0
18	2713.5
19	3374.0
20	4225.5
21	5344.5
22	6718.0
23	8340.0
24	10206.0
25	12422.5
26	15281.5
27	18778.5
28	22649.5
29	27057.5
30	32359.5
31	38237.5
32	44922.5
33	52908.0
34	61357.5
35	70365.5
36	80679.0
37	92099.5
38	105264.0
39	121035.0
40	139014.5
41	161679.0
42	190830.0
43	226549.0
44	272308.0
45	332313.5
46	408532.0
47	504374.0
48	623185.0
49	766260.5
50	932803.5
51	1121735.5
52	1331437.5
53	1558041.0
54	1797930.5
55	2037657.5
56	2267286.5
57	2487277.5
58	2690147.5
59	2869315.5
60	3025907.0
61	3150469.0
62	3239657.5
63	3293037.0
64	3305974.5
65	3271980.0
66	3186405.0
67	3051695.0
68	2866964.0
69	2635015.5
70	2366841.5
71	2078693.5
72	1778726.5
73	1479317.0
74	1195440.0
75	937819.0
76	712505.0
77	521873.0
78	368330.5
79	250361.5
80	163536.5
81	102214.0
82	61102.5
83	35275.5
84	20318.5
85	11666.0
86	6726.5
87	4158.0
88	2716.0
89	1699.0
90	1122.0
91	825.5
92	558.0
93	335.0
94	239.0
95	178.5
96	116.0
97	81.0
98	68.5
99	59.0
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009741555609225306
3	0.014101477033070709
4	0.02234421377139631
5	0.02496599621803663
6	0.031656220471868377
7	0.036614442161477224
8	0.045076555663916086
9	0.03881434621559204
10-14	0.01489430159036955
15-19	0.005786023800906167
20-24	0.0024113034813512337
25-29	0.003029240268657683
30-34	0.011003403834204837
35-39	0.0010053387145433504
40-44	3.4074954066438165E-4
45-49	2.7471102784300902E-5
50-54	3.020862696175426E-4
55-59	6.24616756433003E-4
60-64	0.0
65-69	1.0575533898597072E-4
70-74	0.0
75-79	2.504851553041152E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	40676.0
32-33	42850.0
34-35	44952.0
36-37	46433.0
38-39	46600.0
40-41	47331.0
42-43	47548.0
44-45	48065.0
46-47	49519.0
48-49	49411.0
50-51	38128.0
52-53	34825.0
54-55	41113.0
56-57	46032.0
58-59	51175.0
60-61	54455.0
62-63	58796.0
64-65	62774.0
66-67	67909.0
68-69	73399.0
70-71	79482.0
72-73	87638.0
74-75	95420.0
76-77	105649.0
78-79	115815.0
80-81	128692.0
82-83	143806.0
84-85	164151.0
86-87	193234.0
88-89	228444.0
90-91	286131.0
92-93	364381.0
94-95	486304.0
96-97	663105.0
98-99	1003588.0
100-101	6.004683E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	2.198620551779286
#Duplication Level	Relative count
1	100.0
2	2.135107649695167
3	0.018397289466018672
4	0.015331074555015561
5	0.013797967099514004
6	0.009198644733009336
7	0.006132429822006224
8	0.003066214911003112
9	0.001533107455501556
10++	0.004599322366504668
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE