##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_R1_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	65184661	
Filtered Sequences	0	
Sequence length	30-101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82718311597877	34.0	31.0	34.0	31.0	34.0
2	33.04145042650448	34.0	31.0	34.0	31.0	34.0
3	33.0837646451824	34.0	33.0	34.0	31.0	34.0
4	36.469733055756784	37.0	37.0	37.0	35.0	37.0
5	36.407881924859595	37.0	37.0	37.0	35.0	37.0
6	36.3883034537834	37.0	37.0	37.0	35.0	37.0
7	36.382591158984475	37.0	37.0	37.0	35.0	37.0
8	36.3659400483804	37.0	37.0	37.0	35.0	37.0
9	38.167514210129895	39.0	39.0	39.0	37.0	39.0
10-14	38.40988851963194	39.4	38.8	39.4	37.0	39.4
15-19	39.57410256686002	41.0	40.0	41.0	37.0	41.0
20-24	39.31009162416293	40.8	39.0	41.0	36.2	41.0
25-29	37.67817165145647	40.0	37.6	41.0	28.4	41.0
30-34	37.22389098308696	39.8	36.2	41.0	31.4	41.0
35-39	37.07864382341525	38.6	35.0	40.6	32.2	41.0
40-44	35.322193657764494	36.6	34.6	40.0	26.6	41.0
45-49	35.4038058014515	35.0	34.8	38.8	30.8	41.0
50-54	34.68369234694319	35.0	34.6	36.6	30.6	40.0
55-59	33.95033744674089	35.0	34.0	35.0	30.0	38.0
60-64	33.81948992879842	35.0	34.0	35.0	30.4	36.2
65-69	33.56016959630804	35.0	33.4	35.0	30.0	35.0
70-74	33.191041604904115	35.0	33.0	35.0	29.2	35.0
75-79	32.903553662431804	34.4	32.6	35.0	28.8	35.0
80-84	33.24839019624528	35.0	33.0	35.0	29.0	35.0
85-89	33.1024499397318	35.0	33.0	35.0	29.0	35.0
90-94	33.135537591139624	35.0	33.0	35.0	29.0	35.0
95-99	33.21382787056669	35.0	33.0	35.0	29.0	35.0
100-101	32.8111396773148	34.0	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	5.0
20	124.0
21	1639.0
22	11763.0
23	47191.0
24	126457.0
25	253226.0
26	424952.0
27	626773.0
28	855581.0
29	1120398.0
30	1445986.0
31	1918418.0
32	2718553.0
33	4182218.0
34	6734369.0
35	1.2166099E7
36	2.054023E7
37	1.0436064E7
38	1441476.0
39	132719.0
40	416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.45456065193006	24.69374658556558	13.763165846639902	23.088526915864456
2	30.12199669820953	24.661289417706776	19.9354577941706	25.281256089913096
3	28.98570396733538	20.054844807193742	22.194395803994247	28.76505542147663
4	30.305050670981633	20.281049006540588	20.594902057150083	28.818998265327693
5	30.999320530331513	21.122090364173253	20.30665465913829	27.571934446356945
6	29.32281568511954	19.54173237166946	17.791139544317026	33.34431239889397
7	32.44136530832	19.194498840762552	19.63311429969698	28.731021551220465
8	28.339024421711727	19.85735877340836	20.03337564338948	31.770241161490432
9	30.498297751368224	18.237865807110666	19.400358621179297	31.863477820341814
10-14	30.423894926845552	19.669376440228213	19.608397038990542	30.29833159393569
15-19	30.650096228314183	19.197924611116253	19.400649606205054	30.751329554364514
20-24	30.635164712657172	19.241419792901198	19.377748805541373	30.745666688900265
25-29	31.571779022361458	19.176247645887923	19.091192214814512	30.160781116936107
30-34	31.295970807692946	19.13353566889093	19.002522014312724	30.567971509103405
35-39	30.840451462706397	19.205545973021295	19.243411107385757	30.710591456886547
40-44	31.65066115985421	19.126312085627937	18.9795812222765	30.243445532241353
45-49	31.018400235897747	19.118851698878235	19.068285692031424	30.794462373192594
50-54	30.871189061345998	19.201283492922467	19.201893046540757	30.72563439919077
55-59	30.88891720679441	19.314844645428963	19.189826957210613	30.606411190566014
60-64	30.968169440770772	19.090666793487568	19.055650350227683	30.885513415513977
65-69	30.756662172111916	19.20031061755565	19.234210957240837	30.808816253091592
70-74	30.869368661884643	19.331665066770455	19.194131051145074	30.60483522019982
75-79	30.93752979958285	19.134272131912606	19.08645149003402	30.84174657847052
80-84	30.713393311584248	19.208551021564872	19.263482529550522	30.81457313730035
85-89	30.783291850201977	19.35082267151387	19.255488212292768	30.610397265991384
90-94	30.824647151030206	19.155825084162668	19.153885554346648	30.865642210460482
95-99	30.567892624788897	19.245669049581196	19.365143431033957	30.82129489459595
100-101	31.037922739596123	19.2210891714482	19.17692080743407	30.564067281521606
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.543087567794515
2	55.40325278812263
3	57.750759388812014
4	59.12404893630933
5	58.57125497668846
6	62.66712808401351
7	61.172386859540474
8	60.10926558320215
9	62.36177557171004
10-14	60.72222652078124
15-19	61.4014257826787
20-24	61.38083140155744
25-29	61.73256013929757
30-34	61.86394231679635
35-39	61.551042919592945
40-44	61.89410669209556
45-49	61.81286260909034
50-54	61.596823460536775
55-59	61.495328397360424
60-64	61.853682856284756
65-69	61.565478425203516
70-74	61.47420388208447
75-79	61.779276378053375
80-84	61.5279664488846
85-89	61.393689116193364
90-94	61.69028936149068
95-99	61.38918751938485
100-101	61.60199002111773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	131.0
1	109.5
2	85.0
3	82.0
4	79.5
5	94.5
6	111.0
7	133.5
8	177.0
9	216.5
10	282.5
11	399.5
12	551.0
13	708.0
14	914.5
15	1244.0
16	1639.5
17	2068.5
18	2573.0
19	3254.5
20	4108.5
21	5235.0
22	6589.0
23	8081.0
24	9941.5
25	12213.5
26	15003.5
27	18279.5
28	22157.5
29	26828.5
30	32024.5
31	37758.0
32	44690.5
33	52660.0
34	61285.5
35	70747.0
36	81122.5
37	92785.0
38	106038.5
39	121431.0
40	139598.5
41	162098.0
42	190036.0
43	226036.5
44	273017.5
45	332904.5
46	409635.5
47	505595.5
48	624270.0
49	768478.0
50	935480.0
51	1126597.5
52	1340250.5
53	1568519.0
54	1807692.5
55	2049047.0
56	2283617.0
57	2503754.0
58	2705011.0
59	2887047.0
60	3042410.0
61	3168332.0
62	3260048.5
63	3312685.0
64	3321573.5
65	3282336.5
66	3194832.5
67	3054713.5
68	2863615.0
69	2631119.5
70	2362549.0
71	2068993.5
72	1767374.5
73	1468478.5
74	1183384.5
75	925479.0
76	701837.5
77	513148.0
78	361356.0
79	244765.5
80	159646.0
81	100294.0
82	60450.0
83	35146.0
84	20356.5
85	11952.5
86	7004.5
87	4371.0
88	2810.0
89	1731.5
90	1101.0
91	781.0
92	518.0
93	294.0
94	199.0
95	126.0
96	57.5
97	35.0
98	26.0
99	15.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.2954890875324183E-5
3	4.602309736641876E-6
4	2.3011548683209385E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.0193505033339055E-5
15-19	6.136412982189169E-7
20-24	0.0015807399842119301
25-29	0.2072294277943702
30-34	0.25967369047481625
35-39	0.01870469988649288
40-44	0.3376198262986573
45-49	9.259025325983584E-7
50-54	6.738753849412628E-4
55-59	0.10450370854473146
60-64	0.01242391652505457
65-69	0.011910134972199159
70-74	6.56925512398615E-5
75-79	0.0
80-84	3.7785770502073347E-4
85-89	0.0018565921602187404
90-94	0.006810182141486887
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	39846.0
32-33	40514.0
34-35	41813.0
36-37	45468.0
38-39	49384.0
40-41	48233.0
42-43	51628.0
44-45	42237.0
46-47	46401.0
48-49	51392.0
50-51	55938.0
52-53	59372.0
54-55	59669.0
56-57	62275.0
58-59	65837.0
60-61	69102.0
62-63	71836.0
64-65	77700.0
66-67	85350.0
68-69	92088.0
70-71	93411.0
72-73	98058.0
74-75	111216.0
76-77	75614.0
78-79	106571.0
80-81	127828.0
82-83	151070.0
84-85	170407.0
86-87	211279.0
88-89	222870.0
90-91	279305.0
92-93	364355.0
94-95	489785.0
96-97	784761.0
98-99	1111227.0
100-101	5.9630821E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	1.8102375155602335
#Duplication Level	Relative count
1	100.0
2	1.7662213351798297
3	0.016292615372082604
4	0.017820048063215348
5	0.01120117306830679
6	0.005600586534153395
7	0.0035640096126430694
8	0.004073153843020651
9	0.0015274326911327441
10++	0.0025457211518879067
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE