##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	973637	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21391648016663	33.0	31.0	34.0	30.0	34.0
2	32.3685315985321	34.0	31.0	34.0	30.0	34.0
3	32.44250886110532	34.0	31.0	34.0	31.0	34.0
4	35.883950589388036	37.0	35.0	37.0	35.0	37.0
5	35.890239380795926	37.0	35.0	37.0	35.0	37.0
6	35.876289623340114	37.0	36.0	37.0	35.0	37.0
7	35.883511000506346	37.0	36.0	37.0	35.0	37.0
8	35.825400020746955	37.0	36.0	37.0	35.0	37.0
9	37.521466419209624	39.0	38.0	39.0	35.0	39.0
10-14	37.76314807263899	39.4	38.2	39.4	35.0	39.4
15-19	38.784778105187044	40.4	38.4	41.0	34.8	41.0
20-24	38.56828797590889	40.0	38.0	41.0	34.0	41.0
25-29	38.16754190730221	40.0	38.0	41.0	33.2	41.0
30-34	37.53014419131566	39.8	36.6	41.0	32.4	41.0
35-39	36.60455939944764	38.6	35.0	40.4	31.0	41.0
40-44	35.753557845480394	36.6	35.0	40.0	30.6	41.0
45-49	34.81508118528774	35.0	34.4	38.6	29.6	40.8
50-54	33.486796413858556	34.8	33.0	36.2	27.6	39.2
55-59	33.461805580519226	35.0	34.0	35.0	28.4	38.0
60-64	33.118493442628	35.0	33.4	35.0	27.8	36.2
65-69	32.79155681224111	35.0	33.0	35.0	28.4	35.0
70-74	32.51036084290141	35.0	33.0	35.0	26.8	35.0
75-79	32.29772143006069	35.0	33.0	35.0	27.0	35.0
80-84	32.01872812968283	35.0	33.0	35.0	27.0	35.0
85-89	31.731031380278274	35.0	33.0	35.0	24.8	35.0
90-94	31.332197523306945	35.0	32.8	35.0	24.0	35.0
95-99	30.725661617214634	35.0	31.8	35.0	21.8	35.0
100-101	29.454591906429194	34.0	30.0	35.0	9.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1447.0
3	351.0
4	453.0
5	571.0
6	759.0
7	1011.0
8	1224.0
9	1364.0
10	1417.0
11	1529.0
12	1521.0
13	1500.0
14	1521.0
15	1577.0
16	1723.0
17	1916.0
18	2083.0
19	2415.0
20	2872.0
21	3445.0
22	4176.0
23	5074.0
24	6217.0
25	7664.0
26	9285.0
27	11340.0
28	13859.0
29	16628.0
30	20572.0
31	26137.0
32	33214.0
33	45721.0
34	70519.0
35	157589.0
36	320948.0
37	169443.0
38	22592.0
39	1950.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.31706572892942	24.42719232551235	13.558460130243565	23.697281815314664
2	30.43840074957159	24.974110191954434	19.145513049696103	25.44197600877788
3	29.350456958758652	19.943969130627742	21.475212452948043	29.230361457665566
4	30.649525691943236	19.796839589980873	19.925150346510954	29.62848437156494
5	31.104692072772995	21.116844188915586	20.06064758555018	27.717816152761245
6	29.319972925865557	19.45301210016608	17.577850975172115	33.64916399879625
7	32.63185581934882	19.077571795959845	19.401219999609566	28.889352385081768
8	28.427540342137497	19.838035463266664	19.84214571670489	31.892278477890944
9	30.550676616618862	18.25688101681084	19.196652014433667	31.995790352136634
10-14	30.451346255943957	19.676901860809906	19.497699670113462	30.374052213132675
15-19	30.683203192738755	19.21096254728743	19.326063212085202	30.779771047888616
20-24	30.613222606022482	19.243917140231083	19.352654460202306	30.79020579354413
25-29	30.75080420268578	19.34686292129476	19.26630911542019	30.63602376059927
30-34	30.892864554034116	19.181737380644094	19.05887327450923	30.866524790812562
35-39	30.742321188976128	19.211547086100023	19.229153360096788	30.816978364827065
40-44	30.84510572038381	19.28135200481509	19.144705353089442	30.728836921711654
45-49	30.965021335268144	19.047262980495464	19.015915378730796	30.971800305505592
50-54	30.797970970547766	19.135571165792133	19.167783199862935	30.898674663797166
55-59	30.905449417487397	19.236381078174702	19.142566917310223	30.71560258702768
60-64	31.001040428825117	19.055397442784116	18.973929708916156	30.969632419474607
65-69	30.798151672543256	19.147649483329	19.165417912425266	30.88878093170247
70-74	30.887692230266516	19.238134951732526	19.09498098367256	30.7791918343284
75-79	30.99710282109304	19.05911937593156	19.000452735558067	30.943325067417337
80-84	30.79813597230005	19.14247823822021	19.146401685397873	30.912984104081865
85-89	30.9348555981336	19.23875119782835	19.12807339901832	30.698319805019736
90-94	31.011229030942744	19.061580445278885	18.975655197984466	30.951535325793905
95-99	30.826540229063394	19.118387764442176	19.168118957177356	30.88695304931707
100-101	31.014947049054214	19.34273245573042	19.131257337180077	30.511063158035284
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.01434754424409
2	55.880376758349456
3	58.58081841642422
4	60.27801006350817
5	58.82250822553423
6	62.969136924661804
7	61.521208204430586
8	60.319818820028445
9	62.54646696875549
10-14	60.82539846907663
15-19	61.46297424062737
20-24	61.403428399566614
25-29	61.38682796328505
30-34	61.759389344846674
35-39	61.55929955380319
40-44	61.57394264209547
45-49	61.93682164077374
50-54	61.696645634344925
55-59	61.62105200451508
60-64	61.97067284829972
65-69	61.686932604245726
70-74	61.66688406459492
75-79	61.94042788851037
80-84	61.71112007638192
85-89	61.63317540315333
90-94	61.96276435673664
95-99	61.71349327838047
100-101	61.5260102070895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	18.5
2	7.0
3	6.0
4	4.0
5	2.5
6	2.5
7	4.5
8	6.5
9	6.5
10	9.5
11	12.5
12	14.5
13	15.5
14	17.0
15	24.5
16	34.5
17	35.0
18	38.0
19	52.5
20	70.5
21	96.5
22	119.5
23	130.0
24	151.0
25	192.5
26	245.0
27	293.0
28	347.0
29	422.0
30	488.0
31	588.0
32	697.0
33	800.0
34	943.5
35	1047.0
36	1182.5
37	1397.5
38	1556.5
39	1780.5
40	2064.0
41	2366.0
42	2806.0
43	3333.0
44	3979.0
45	4843.0
46	5944.0
47	7340.0
48	8972.0
49	11032.5
50	13581.0
51	16183.5
52	19169.0
53	22608.5
54	26184.5
55	29656.5
56	32987.0
57	36242.0
58	39250.5
59	41948.0
60	44142.0
61	45968.0
62	47397.5
63	48043.5
64	48040.5
65	47609.5
66	46572.0
67	44595.0
68	41913.5
69	38874.5
70	34911.5
71	30447.5
72	26186.0
73	21807.0
74	17491.0
75	13656.5
76	10385.5
77	7610.5
78	5363.5
79	3616.0
80	2303.5
81	1441.0
82	834.5
83	439.5
84	243.5
85	126.0
86	72.0
87	52.0
88	33.0
89	19.5
90	19.5
91	17.5
92	11.0
93	9.0
94	8.5
95	6.5
96	3.0
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02362276700659486
2	0.02886085882110068
3	0.025163382246155396
4	0.022492982497583802
5	0.014789906299781129
6	0.0014379075569231653
7	0.036769350384178084
8	0.04734824169582709
9	0.06511667079209192
10-14	0.0217124041095398
15-19	0.023437893177847596
20-24	0.010085894434989631
25-29	0.012961709548835963
30-34	0.020931825721495795
35-39	0.012366004989539223
40-44	0.003183938161758438
45-49	0.003882350403692547
50-54	0.009017734535560994
55-59	0.0010270768263736896
60-64	0.0
65-69	0.0
70-74	0.0
75-79	2.054153652747379E-5
80-84	3.492061209670544E-4
85-89	0.0
90-94	0.0
95-99	2.670399748571593E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	973637.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.10096112996496519
#Duplication Level	Relative count
1	100.0
2	0.06103814884302689
3	5.003126954346467E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0010006253908692933
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	75430	3.122184	3.7426436	10-14
AAAAA	77550	3.0430026	3.9972937	1
>>END_MODULE