##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57797675	34.0	31.0	34.0	31.0	34.0
2	32.71647325	34.0	31.0	34.0	31.0	34.0
3	32.76925875	34.0	31.0	34.0	31.0	34.0
4	36.1580715	37.0	37.0	37.0	35.0	37.0
5	36.16077	37.0	37.0	37.0	35.0	37.0
6	36.1760235	37.0	37.0	37.0	35.0	37.0
7	36.15374375	37.0	37.0	37.0	35.0	37.0
8	36.12164725	37.0	37.0	37.0	35.0	37.0
9	37.88957875	39.0	39.0	39.0	35.0	39.0
10-14	38.152356149999996	39.4	38.4	39.4	35.4	39.4
15-19	39.2968596	41.0	39.6	41.0	36.4	41.0
20-24	39.0580954	41.0	39.0	41.0	35.6	41.0
25-29	38.698731550000005	40.2	38.4	41.0	35.0	41.0
30-34	38.08407785000001	40.0	37.4	41.0	33.8	41.0
35-39	37.13581965	39.0	35.2	40.8	32.6	41.0
40-44	36.25346095	37.2	35.0	40.0	32.0	41.0
45-49	35.27649269999999	35.2	35.0	39.0	31.0	41.0
50-54	33.92530935	35.0	33.6	36.2	29.4	39.2
55-59	33.95306145000001	35.0	35.0	35.0	30.8	38.2
60-64	33.60485025	35.0	34.4	35.0	30.8	36.4
65-69	33.288741200000004	35.0	34.0	35.0	30.0	35.0
70-74	33.0264987	35.0	34.0	35.0	29.4	35.0
75-79	32.79427025	35.0	33.4	35.0	29.0	35.0
80-84	32.5361759	35.0	33.0	35.0	28.2	35.0
85-89	32.26939885	35.0	33.0	35.0	27.0	35.0
90-94	31.9348012	35.0	33.0	35.0	26.8	35.0
95-99	31.427252899999996	35.0	33.0	35.0	24.4	35.0
100-101	30.406864249999998	34.0	31.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6897.0
3	1218.0
4	1531.0
5	1835.0
6	2378.0
7	2921.0
8	3453.0
9	3971.0
10	4322.0
11	4670.0
12	4746.0
13	4799.0
14	4852.0
15	4905.0
16	5100.0
17	5779.0
18	6503.0
19	7282.0
20	8733.0
21	10402.0
22	12511.0
23	15466.0
24	18721.0
25	23325.0
26	28354.0
27	35295.0
28	42689.0
29	53729.0
30	67362.0
31	85673.0
32	113207.0
33	158623.0
34	258125.0
35	617661.0
36	1416915.0
37	830720.0
38	114888.0
39	10369.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.284630829803	24.425494250814683	13.5386422005863	23.751232718796018
2	30.448338183550362	24.9117103719923	19.092750893654152	25.54720055080319
3	29.379872789928264	19.921797702213357	21.426378890883278	29.2719506169751
4	30.66482888354675	19.830096783796154	19.90686842256671	29.598205910090382
5	31.11798814108344	21.14000569760238	20.101581397303217	27.640424764010962
6	29.309059954226086	19.45498931675229	17.53748139434388	33.698469334677746
7	32.62308774419749	19.010784049275635	19.4665626412188	28.899565565308084
8	28.348263890974394	19.87193155267789	19.885420608273822	31.894383948073894
9	30.512210140574314	18.21158236212904	19.2734490768553	32.002758420441346
10-14	30.43694380470152	19.667894212657284	19.510141912534394	30.3850200701068
15-19	30.665217578584418	19.19255429940462	19.352159771766626	30.790068350244336
20-24	30.60351681222226	19.241077562364694	19.325531432920343	30.829874192492703
25-29	30.746728097709454	19.373977178839784	19.245999174290276	30.63329554916049
30-34	30.880801289321603	19.137219355473277	19.066512588986313	30.915466766218806
35-39	30.764034910720923	19.198345519219593	19.1909276462774	30.846691923782082
40-44	30.855219654724625	19.272563981588274	19.131282320367994	30.740934043319108
45-49	30.984115372463144	19.054691779695	18.99852642842076	30.962666419421097
50-54	30.781878584390814	19.1214890299911	19.15415328704155	30.942479098576538
55-59	30.890672022067022	19.213867299820077	19.121979711220437	30.773480966892464
60-64	31.003324650498698	19.02125785318868	18.978627846794176	30.996789649518448
65-69	30.7832	19.119120000000002	19.145555	30.952125000000002
70-74	30.89550154477508	19.233650961682546	19.094665954733298	30.776181538809077
75-79	30.998829126992344	19.039599128122866	18.98169299026726	30.97987875461753
80-84	30.81404425123832	19.111414956942156	19.13744697963032	30.93709381218921
85-89	30.903101180620236	19.217363843472768	19.110853822170764	30.76868115373623
90-94	31.02973241189296	19.009372603749043	18.971227588491036	30.98966739586696
95-99	30.849745473775393	19.09226696897333	19.12403336987161	30.933954187379666
100-101	31.027705954008205	19.299691683838745	19.09061420104364	30.581988161109415
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.03586354859901
2	55.995538734353545
3	58.65182340690337
4	60.26303479363714
5	58.7584129050944
6	63.00752928890383
7	61.52265330950557
8	60.24264783904829
9	62.514968561015664
10-14	60.82196387480832
15-19	61.45528592882875
20-24	61.43339100471496
25-29	61.380023646869944
30-34	61.7962680555404
35-39	61.610726834503005
40-44	61.59615369804373
45-49	61.94678179188424
50-54	61.72435768296736
55-59	61.664152988959486
60-64	62.00011430001714
65-69	61.735324999999996
70-74	61.67168308358415
75-79	61.97870788160987
80-84	61.751138063427526
85-89	61.671782334356465
90-94	62.019399807759925
95-99	61.783699661155055
100-101	61.60969411511762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	140.0
1	94.5
2	43.5
3	33.5
4	33.0
5	32.0
6	28.5
7	32.5
8	39.5
9	44.5
10	43.0
11	52.5
12	71.5
13	84.5
14	109.0
15	138.0
16	164.0
17	198.0
18	242.5
19	292.0
20	348.5
21	414.5
22	532.5
23	651.5
24	712.5
25	840.0
26	1041.5
27	1258.0
28	1483.0
29	1700.0
30	1987.5
31	2400.0
32	2789.0
33	3249.0
34	3821.0
35	4378.5
36	5019.0
37	5690.5
38	6396.5
39	7317.5
40	8387.5
41	9760.5
42	11474.0
43	13507.0
44	16217.5
45	19694.0
46	24084.0
47	29848.5
48	37025.0
49	45528.5
50	55457.5
51	67107.5
52	79405.0
53	92509.5
54	106944.0
55	121512.0
56	135588.0
57	148613.5
58	160370.5
59	171571.0
60	180835.5
61	187756.0
62	193651.5
63	196825.5
64	198125.0
65	196410.5
66	191010.5
67	182997.0
68	172372.0
69	159129.5
70	143015.5
71	125328.5
72	106938.5
73	89014.5
74	72451.5
75	57162.5
76	43514.5
77	31864.0
78	22415.5
79	15378.0
80	10065.0
81	6234.5
82	3639.0
83	2021.5
84	1128.0
85	663.5
86	415.0
87	280.0
88	203.0
89	155.0
90	123.5
91	105.0
92	80.0
93	52.5
94	37.5
95	31.5
96	25.0
97	22.0
98	18.5
99	14.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06845
2	0.0732
3	0.06687499999999999
4	0.06075
5	0.045675
6	0.030400000000000003
7	0.08855
8	0.1042
9	0.124
10-14	0.05534
15-19	0.056685000000000006
20-24	0.034185
25-29	0.03751
30-34	0.05907
35-39	0.03873
40-44	0.02241
45-49	0.018430000000000002
50-54	0.02834
55-59	0.0137
60-64	1.4999999999999999E-5
65-69	0.0
70-74	4.9999999999999996E-6
75-79	0.001965
80-84	0.007770000000000001
85-89	1.9999999999999998E-5
90-94	3.9999999999999996E-5
95-99	0.00441
100-101	0.0011875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.26530897753618193
#Duplication Level	Relative count
1	100.0
2	0.18485769964882046
3	0.003005816254452365
4	5.009693757420609E-4
5	0.0010019387514841219
6	0.0
7	5.009693757420609E-4
8	0.0
9	5.009693757420609E-4
10++	0.0035067856301944263
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	345235	3.2958472	4.1731076	1
TTTTT	310755	3.133097	3.7799935	10-14
>>END_MODULE