##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4652925	34.0	31.0	34.0	31.0	34.0
2	32.63597725	34.0	31.0	34.0	31.0	34.0
3	32.67428525	34.0	31.0	34.0	31.0	34.0
4	36.0818575	37.0	37.0	37.0	35.0	37.0
5	36.02419425	37.0	37.0	37.0	35.0	37.0
6	35.9943015	37.0	37.0	37.0	35.0	37.0
7	36.03466675	37.0	37.0	37.0	35.0	37.0
8	36.03513675	37.0	37.0	37.0	35.0	37.0
9	37.772766	39.0	38.0	39.0	35.0	39.0
10-14	38.0055282	39.4	38.2	39.4	35.2	39.4
15-19	39.1236186	41.0	39.0	41.0	36.0	41.0
20-24	38.8561577	40.6	39.0	41.0	35.2	41.0
25-29	38.517968800000006	40.0	38.0	41.0	34.6	41.0
30-34	37.9216392	40.0	37.0	41.0	33.2	41.0
35-39	37.0015669	38.8	35.2	40.8	32.6	41.0
40-44	36.092790550000004	37.0	35.0	40.0	31.4	41.0
45-49	34.854034799999994	35.0	34.6	38.8	29.8	41.0
50-54	33.7243703	34.8	33.2	36.2	28.8	39.2
55-59	33.781249100000004	35.0	34.0	35.0	30.0	38.0
60-64	33.41043675	35.0	34.0	35.0	30.0	36.2
65-69	33.09580845	35.0	34.0	35.0	29.2	35.0
70-74	32.821838449999994	35.0	33.4	35.0	29.0	35.0
75-79	32.559734000000006	35.0	33.0	35.0	28.0	35.0
80-84	32.27447600000001	35.0	33.0	35.0	27.0	35.0
85-89	31.977888749999998	35.0	33.0	35.0	26.6	35.0
90-94	31.565286199999996	35.0	33.0	35.0	24.4	35.0
95-99	30.9827182	35.0	32.4	35.0	23.0	35.0
100-101	29.7637435	34.0	30.5	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8200.0
3	1295.0
4	1729.0
5	2043.0
6	2670.0
7	3329.0
8	3984.0
9	4545.0
10	4903.0
11	5310.0
12	5380.0
13	5477.0
14	5464.0
15	5570.0
16	5915.0
17	6402.0
18	7317.0
19	8399.0
20	9895.0
21	11676.0
22	14372.0
23	17601.0
24	21008.0
25	25774.0
26	31680.0
27	38460.0
28	47326.0
29	59086.0
30	73543.0
31	94103.0
32	124277.0
33	175981.0
34	285352.0
35	654682.0
36	1356093.0
37	758757.0
38	103306.0
39	9037.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.35355639375231	24.42042447784721	13.556568897896376	23.669450230504108
2	30.470249243985354	24.90593138377268	19.142328437536364	25.481490934705608
3	29.379982085758176	19.971177085783197	21.402765198332673	29.24607563012595
4	30.619553192873912	19.866448418955514	19.90909682489626	29.604901563274318
5	31.21761810317032	21.125234723023706	20.05019264359846	27.60695453020751
6	29.356309951573195	19.548655572987116	17.51261730009301	33.58241717534668
7	32.59290861752418	18.976985567814598	19.48734357704781	28.94276223761342
8	28.321299865014787	19.849888430590305	19.89929952943003	31.929512174964874
9	30.552932133897436	18.240481737854687	19.242140441591363	31.96444568665651
10-14	30.498326499612748	19.66182654903575	19.500154525082486	30.339692426269018
15-19	30.692985662710758	19.205462375476102	19.331707914716972	30.769844047096168
20-24	30.62666820046581	19.24001086201997	19.3313833268794	30.80193761063481
25-29	30.757794096389862	19.367859394576676	19.278733401203112	30.595613107830346
30-34	30.887222964621543	19.12726241450548	19.087370060793543	30.898144560079437
35-39	30.713352223049643	19.191522276113446	19.2171268317336	30.877998669103306
40-44	30.853904981218278	19.252460073333957	19.156820570327202	30.736814375120563
45-49	30.97308438284388	19.05046148653539	19.07120886118971	30.90524526943102
50-54	30.755172107948493	19.12500968615749	19.171218097649174	30.948600108244843
55-59	30.861722464339834	19.20358175084138	19.129397203020414	30.805298581798368
60-64	31.0043708515298	19.046626666319334	18.965041637764575	30.983960844386292
65-69	30.796431645905002	19.11430050148698	19.146505628697234	30.942762223910787
70-74	30.89400930783444	19.21623610007357	19.103000681102518	30.78675391098947
75-79	30.996533882151994	19.02217675400964	18.991785720714503	30.989503643123868
80-84	30.800115066039446	19.12773860724682	19.12433783765266	30.947808489061075
85-89	30.93181237272495	19.213557685423073	19.084432633773055	30.770197308078924
90-94	31.030324648519436	19.031380450208722	18.95732033171253	30.98097456955931
95-99	30.830711112013553	19.104963237555697	19.143037501024	30.92128814940675
100-101	31.06867961440339	19.344466756632208	19.070331899835697	30.51652172912871
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	62.02300662425642
2	55.951740178690955
3	58.62605771588413
4	60.224454756148226
5	58.824572633377834
6	62.93872712691988
7	61.53567085513759
8	60.250812039979664
9	62.51737782055395
10-14	60.83801892588176
15-19	61.46282970980692
20-24	61.428605811100624
25-29	61.35340720422021
30-34	61.78536752470097
35-39	61.59135089215295
40-44	61.59071935633884
45-49	61.8783296522749
50-54	61.70377221619333
55-59	61.6670210461382
60-64	61.9883316959161
65-69	61.73919386981579
70-74	61.680763218823905
75-79	61.98603752527586
80-84	61.74792355510051
85-89	61.70200968080387
90-94	62.011299218078754
95-99	61.751999261420295
100-101	61.58520134353209
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	98.0
1	67.0
2	24.5
3	16.0
4	17.5
5	18.0
6	18.5
7	26.5
8	33.0
9	30.0
10	45.0
11	59.5
12	84.0
13	120.5
14	154.0
15	188.0
16	235.0
17	289.5
18	335.5
19	385.0
20	447.0
21	511.5
22	576.5
23	649.5
24	788.5
25	918.0
26	1066.5
27	1284.0
28	1520.5
29	1806.0
30	2111.5
31	2417.0
32	2811.5
33	3302.0
34	3777.5
35	4331.0
36	4994.0
37	5650.0
38	6384.5
39	7354.0
40	8424.5
41	9755.5
42	11550.5
43	13657.5
44	16287.5
45	19925.5
46	24314.0
47	29881.0
48	37036.0
49	45483.0
50	55331.5
51	66605.5
52	79248.5
53	93160.5
54	107598.5
55	121483.5
56	135131.0
57	148659.5
58	160879.0
59	171266.0
60	180795.0
61	188438.5
62	193726.0
63	197311.5
64	198516.5
65	196075.0
66	190831.0
67	183318.0
68	172018.5
69	157978.0
70	142333.5
71	125188.0
72	107121.5
73	89365.0
74	72315.5
75	56489.5
76	42904.0
77	31618.0
78	22411.5
79	15144.5
80	9831.5
81	6197.5
82	3704.5
83	2152.0
84	1245.0
85	717.5
86	440.0
87	307.0
88	225.0
89	167.0
90	135.0
91	106.0
92	87.0
93	70.0
94	42.0
95	29.5
96	25.0
97	19.5
98	14.5
99	6.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08312499999999999
2	0.092875
3	0.0795
4	0.11349999999999999
5	0.055075
6	0.1173
7	0.148525
8	0.17425
9	0.178075
10-14	0.097125
15-19	0.09548
20-24	0.073835
25-29	0.068435
30-34	0.10617
35-39	0.03732
40-44	0.05176
45-49	0.035545
50-54	0.050665
55-59	0.06005
60-64	3.5E-5
65-69	3.95E-4
70-74	3.7E-4
75-79	0.0034
80-84	0.02263
85-89	3.9999999999999996E-5
90-94	1.5999999999999999E-4
95-99	0.02433
100-101	0.017275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.19337044180398807
#Duplication Level	Relative count
1	100.0
2	0.1196513589690959
3	0.0030037998067555458
4	5.006333011259243E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.00350443310788147
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	316940	3.1928525	3.8381712	10-14
AAAAA	328140	3.1414685	4.092738	1
>>END_MODULE