##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002006_Sedgewick_ACTGAT_L003_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55280675	34.0	31.0	34.0	31.0	34.0
2	32.714679	34.0	31.0	34.0	31.0	34.0
3	32.76547225	34.0	31.0	34.0	31.0	34.0
4	36.1433425	37.0	37.0	37.0	35.0	37.0
5	36.1162665	37.0	37.0	37.0	35.0	37.0
6	36.14436325	37.0	37.0	37.0	35.0	37.0
7	36.14649025	37.0	37.0	37.0	35.0	37.0
8	36.1252785	37.0	37.0	37.0	35.0	37.0
9	37.8805755	39.0	39.0	39.0	35.0	39.0
10-14	38.0675011	39.4	38.6	39.4	35.4	39.4
15-19	39.27764155	41.0	39.8	41.0	36.4	41.0
20-24	38.5777581	40.6	38.6	41.0	34.4	41.0
25-29	38.58075715	40.0	38.0	41.0	35.0	41.0
30-34	38.05859585	40.0	37.4	41.0	33.8	41.0
35-39	36.8896793	38.8	35.2	40.6	32.0	41.0
40-44	36.046733849999995	37.0	35.0	40.0	31.4	41.0
45-49	35.11629675	35.2	35.0	38.8	31.0	41.0
50-54	33.83133084999999	35.0	33.2	36.2	28.8	39.2
55-59	33.8712853	35.0	34.6	35.0	30.2	38.2
60-64	33.492559500000006	35.0	34.0	35.0	30.0	36.4
65-69	33.1626479	35.0	34.0	35.0	29.8	35.0
70-74	32.8729819	35.0	33.8	35.0	29.0	35.0
75-79	32.468766	35.0	33.0	35.0	27.0	35.0
80-84	32.30581995	35.0	33.0	35.0	27.0	35.0
85-89	32.02291395	35.0	33.0	35.0	27.0	35.0
90-94	31.6148734	35.0	33.0	35.0	24.6	35.0
95-99	31.017242450000005	35.0	32.6	35.0	23.0	35.0
100-101	29.887228625	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9346.0
3	1368.0
4	1807.0
5	2207.0
6	2797.0
7	3269.0
8	3966.0
9	4516.0
10	4756.0
11	5101.0
12	5054.0
13	5276.0
14	5450.0
15	5504.0
16	5782.0
17	6429.0
18	7232.0
19	8183.0
20	9470.0
21	11393.0
22	13453.0
23	16691.0
24	19824.0
25	24525.0
26	29432.0
27	36191.0
28	44843.0
29	55755.0
30	69725.0
31	89378.0
32	119572.0
33	170095.0
34	286952.0
35	676135.0
36	1371609.0
37	754836.0
38	103134.0
39	8871.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.279047311795125	24.497399932373465	13.561122800729725	23.662429955101686
2	30.43797109393283	24.888551830813533	19.1350205426051	25.53845653264854
3	29.386538641050862	19.977641898491616	21.416828938500707	29.218990521956812
4	30.59668932309326	19.858604473261234	19.98048508433142	29.564221119314084
5	31.15750711717102	21.080613894629586	20.150608522188282	27.611270466011113
6	29.339065946744658	19.45418706192634	17.52594967787211	33.68079731345689
7	32.59043002699434	19.02867948029894	19.475948406372908	28.90494208633382
8	28.3119966269877	19.910687281300014	19.90875941649291	31.868556675219377
9	30.55007966163274	18.213030454706043	19.29346404034086	31.943425843320355
10-14	30.472703353781416	19.711321398105696	19.499062135840123	30.316913112272765
15-19	30.674029644541534	19.198902589285975	19.352743061390363	30.774324704782124
20-24	30.818974693687217	19.231246668065943	19.277492849553813	30.672285788693028
25-29	30.703122373306307	19.395651315968536	19.296387310537835	30.604839000187322
30-34	30.89334957544625	19.137830076402658	19.098817975895077	30.870002372256018
35-39	30.84561882388813	19.246490468401387	19.181551291545844	30.726339416164638
40-44	30.887358977921064	19.31982533595195	19.12039251836284	30.67242316776414
45-49	30.97514356603187	19.077577928521457	19.01137593796991	30.93590256747676
50-54	30.771618536317863	19.141349181221198	19.165721722112114	30.921310560348825
55-59	30.885596518655422	19.22011745930846	19.14185194399803	30.752434078038082
60-64	30.99816109429939	19.035799010976934	19.0008839027401	30.965155991983572
65-69	30.791410072082737	19.128092166299542	19.151677242951042	30.928820518666683
70-74	30.88895145611553	19.231942125882025	19.121716139359446	30.757390278642994
75-79	31.07016827242685	19.035044809127125	18.98295204560602	30.911834872840004
80-84	30.808594303076614	19.11250223621905	19.151707651137524	30.92719580956681
85-89	30.918121570740396	19.212658795444625	19.11364351820185	30.755576115613124
90-94	31.033965000000002	19.02461	18.970735	30.97069
95-99	30.851175350508562	19.113334669709122	19.12272731091983	30.912762668862488
100-101	31.00428524310509	19.324465944982748	19.12491970014051	30.54632911177165
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	61.941477266896804
2	55.97642762658137
3	58.605529163007674
4	60.16091044240734
5	58.76877758318213
6	63.01986326020155
7	61.49537211332815
8	60.18055330220709
9	62.49350550495309
10-14	60.789616466054184
15-19	61.448354349323665
20-24	61.49126048238025
25-29	61.307961373493626
30-34	61.76335194770226
35-39	61.57195824005277
40-44	61.559782145685205
45-49	61.91104613350863
50-54	61.692929096666695
55-59	61.638030596693504
60-64	61.96331708628296
65-69	61.72023059074942
70-74	61.64634173475852
75-79	61.98200314526686
80-84	61.73579011264342
85-89	61.67369768635352
90-94	62.004655
95-99	61.76393801937105
100-101	61.55061435487674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	80.0
2	47.5
3	38.0
4	36.0
5	30.0
6	35.0
7	41.5
8	42.5
9	57.5
10	75.0
11	96.5
12	128.5
13	151.0
14	187.5
15	227.5
16	262.0
17	300.0
18	353.0
19	389.5
20	423.5
21	501.0
22	595.0
23	716.5
24	807.5
25	897.5
26	1101.0
27	1302.5
28	1495.5
29	1768.5
30	2069.5
31	2406.0
32	2823.0
33	3319.0
34	3836.5
35	4414.5
36	5058.0
37	5712.5
38	6510.0
39	7465.0
40	8459.5
41	9696.5
42	11361.0
43	13511.5
44	16236.5
45	19805.5
46	24428.0
47	29812.0
48	36884.0
49	45624.0
50	55381.5
51	66896.0
52	79680.0
53	93280.0
54	107769.5
55	122190.5
56	135447.0
57	148296.5
58	160859.5
59	171584.0
60	181189.0
61	188972.0
62	194010.5
63	197361.5
64	198113.0
65	195743.0
66	190813.0
67	182821.0
68	171662.0
69	158023.5
70	141745.5
71	124163.0
72	106542.0
73	88734.5
74	71661.5
75	56472.5
76	43057.0
77	31620.5
78	22447.5
79	15199.0
80	9992.0
81	6331.0
82	3787.5
83	2283.0
84	1361.5
85	786.5
86	515.0
87	362.0
88	260.5
89	200.0
90	154.5
91	127.5
92	107.0
93	84.0
94	62.0
95	51.0
96	48.0
97	37.0
98	19.0
99	13.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.113175
2	0.11612499999999999
3	0.10332500000000001
4	0.127675
5	0.091975
6	0.10955
7	0.12719999999999998
8	0.14859999999999998
9	0.155825
10-14	0.08681
15-19	0.08481
20-24	0.067425
25-29	0.06448
30-34	0.0951
35-39	0.03723
40-44	0.051555000000000004
45-49	0.04077
50-54	0.051455
55-59	0.058155
60-64	3.1E-4
65-69	3.25E-4
70-74	8.950000000000001E-4
75-79	0.005305
80-84	0.026565
85-89	2.8E-4
90-94	0.0
95-99	0.02812
100-101	0.023175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.20542501986775313
#Duplication Level	Relative count
1	100.0
2	0.16728270777614168
3	0.00150253929140247
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	5.008464304674901E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	323260	3.2516916	3.6426973	10-14
AAAAA	336275	3.2049613	3.9642167	1
>>END_MODULE